小麦麸皮多酚对肥胖大鼠的减肥降脂作用. (Chinese)

This study aimed to observe the antiobesity and lipid-lowering effects of wheat bran polyphenols (WBP) in diet-induced obese rats, and to explore its possible mechanism. Eighty 6-week-old SD rats, half from each gender, were adaptively fed for one week. Ten rats were randomly selected from them and fed on a basal diet to serve as a blank control group. The remaining 70 rats were fed on a high-fat diet until the body mass gain exceeded 20%. Totally 50 obese rats were selected from the 70 rats and randomly divided into five groups of 10 rats each: model (distilled water 5.0 mL/kg m b ), positive control (trimethylglycine (TMG), 0.10 g/kg m b ), low-dose WBP (WBP-L, 1.50 g/kg m b ), medium-dose WBP (WBP-M, 3.00 g/kg m b ), and high-dose WBP (WBP-H, 6.00 g/kg m b ). Each drug was administered daily at a dose of 5.0 mL/kg m b by gavage. The rats in the blank control group were gavaged with distilled water (5.0 mL/kg m b ) for nine weeks. During the intervention period, body mass and food intake of the rats were measured once a week and food effect was calculated. After completion of the intervention period, the rats were fasted for 12 hours with access to water and then anesthetized with ether to collect blood samples from the abdominal aorta and liver and surrounding adipose tissue to calculate liver coefficients and obesity indexes. Sera were separated from the blood samples for measurement of biochemical indicators. Liver tissues were homogenized for analyses of antiinflammatory and antioxidant parameters. Moreover, the degree of hepatic steatosis was pathohistologically observed. The results showed that WBP at all doses could reduce the food effect, inhibit the body mass gain in obese rats, reduce the liver mass and liver coefficient, and significantly lower the levels of triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatinine (CREA) in the sera of obese rats. In addition, all doses of WBP decreased the levels of interleukin-6 (IL-6), IL1β. tumor necrosis factor-α (TNF-α), adiponectin (ADPN) and leptin (LEP) in the liver homogenate of obese rats to varying degrees, but significantly increased glutathione peroxidase (GSH-Px), lipoprotein lipase (LPL), and hepaticlipase (HL) levels (P < 0.05 or P < 0.01); the effect of WBP was more pronounced than that of TMG. In conclusion, WBP can exert antiobesity, lipid-lowering and hepatoprotective functions by inhibiting the degree of obesity in simple obesity rats, reducing serum lipid levels, alleviating chronic inflammation caused by obesity, and inhibiting liver injury. Therefore, WBP has the potential to be developed as a functional food and health product with antiobesity and lipid-lowering effects. This study provides a theoretical reference for scientific and reasonable development of wheat bran. [ABSTRACT FROM AUTHOR]

目的：观察小麦麸皮多酚 (wheat bran polyphenol, WBP) 对饮食诱导肥胖大鼠的减肥降脂作用, 并 探讨其可能的作用机制。方法：80 只6 周龄SD大鼠, 雌雄各半, 适应性饲养一周后, 随机抽取10 只作为空白 对照组饲以基础饲料, 其余70 只作为模型组饲以高脂饲料, 以大鼠体质量增长率超过20%认为造模成功。筛选 成模大鼠50 只, 随机分为5 组：模型组 (蒸馏水5.0 mL/kg m b ), 阳性对照甜菜碱 (trimethylglycine, TMG) (0.10 g/kg m b )组, WBP低 (WBP-L) (1.50 g/kg m b )、中 (WBP-M) (3.00 g/kg m b )、高 (WBP-H) (6.00 g/kg m b ) 剂量组, 每组10 只, 每日按5.0 mL/kg m b 灌胃给予受试物；空白对照组灌以蒸馏水 (5.0 mL/kg m b ), 干预9 周。干预期间每周测量体质量1 次, 每周测定进食量并计算食物效应比。干预末期, 大鼠禁食不禁水12 h后乙醚麻醉, 腹主动脉采集血样, 并取肝脏和周围脂肪组织, 称质量, 计算肝脏系数、肥胖指数。从血液中分离血清测定血生 化指标；取一定量肝组织进行匀浆, 测定抗炎、抗氧化活性等相关指标。取部分肝脏进行病理组织学分析, 观察 肝组织脂肪变性的程度。结果：各剂量WBP均可降低肥胖大鼠食物效应比, 抑制大鼠体质量增长；降低肥胖大鼠 肝脏质量及肝脏系数。各剂量WBP均可明显降低肥胖大鼠血清中甘油三酯 (triglyceride, TG)、总胆固醇 (total cholesterol, TC)、高密度脂蛋白胆固醇 (high density lipoprotein cholesterol, HDL-C)、低密度脂蛋白胆固醇 (low density lipoprotein cholesterol, LDL-C)、碱性磷酸酶 (alkaline phosphatase, ALP)、丙氨酸氨基转移酶 (alanine aminotransferase, ALT)、天冬氨酸氨基转移酶 (aspartate aminotransferase, AST)、肌酐 (creatinine, CREA)水平；此外, 各剂量WBP均可不同程度降低肥胖大鼠肝匀浆中白细胞介素 (interleukin, IL)-6、IL-1β、肿瘤坏死因子 (tumor necrosis factor, TNF-α)、脂联素 (adiponectin, ADPN)、瘦素 (leptin, LEP)水平, 显 著提升谷胱甘肽过氧化物酶 (glutathione peroxidase, GSH-Px)、脂蛋白脂酶 (lipoprotein lipase, LPL)、肝脂酶 (hepaticlipase, HL)水平 (P＜0.05, P＜0.01), 且效果均优于阳性对照TMG。结论：WBP可抑制单纯性肥胖大鼠 肥胖程度, 降低血脂水平, 减轻肥胖引起的慢性炎症, 抑制肝损伤, 从而起到减肥、降脂、保肝的作用。综上, WBP 具有开发成为减肥降脂功能性食品及保健品的潜力, 本研究可为科学合理开发小麦麸皮提供理论参考和依据。 [ABSTRACT FROM AUTHOR]

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