The Xbal-Blnl-Ceul genomic cleavage map of Salmonella enteritidis shows an inversion relative to Salmonella typhimurium LT2.
In: Molecular Microbiology, Jg. 10 (1993-11-01), Heft 3, S. 655-664
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Zugriff:
We have established the genomic cleavage map of Salmonella enteritidis strain SSU7998 using pulsed-field gel electrophoresis. The chromosome of 4600 kb was analysed by Xba l (16 fragments), I Ceu l (7 fragments) and Bln l (12 fragments); the genome also contains a plasmid of 60 kb. Cleavage sites of I- Ceu l, in the large subunit ribosomal RNA gene, are conserved from Salmonella typhimurium and Escherichia coli K-12, and the Xba l and Bln l sites in glt-tRNA are also conserved, but other sites are less conserved. Transposon Tn 10 , located at 60 different positions in the chromosome of S. typhimurium , was transduced by bacteriophage P22 into S. enteritidis and the insertion mapped using the Xba l and Bln l sites on Tn 10 . Gene order in S. enteritidis is identical to S. typhimurium LT2 and similar to E. coli K-12 except for an inversion of 815 kb, which covers the terminus region including T1 and T2. Endpoints are in the NDZs, or non-divisible zones, in which inversion endpoints were not detected in experiments in E. coli K-12 and S. typhimurium LT2. This inversion resembles the inversion between S. typhimurium and E. coli , but is longer at both ends. [ABSTRACT FROM AUTHOR]
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The Xbal-Blnl-Ceul genomic cleavage map of Salmonella enteritidis shows an inversion relative to Salmonella typhimurium LT2.
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Autor/in / Beteiligte Person: | Liu, Shu-Lin ; Hessel, Andrew ; Sanderson, Kenneth E. |
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Zeitschrift: | Molecular Microbiology, Jg. 10 (1993-11-01), Heft 3, S. 655-664 |
Veröffentlichung: | 1993 |
Medientyp: | academicJournal |
ISSN: | 0950-382X (print) |
DOI: | 10.1111/j.1365-2958.1993.tb00937.x |
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