Translation elongation factor EF-Tu is a target for Stp, a serine-threonine phosphatase involved in virulence ofListeria monocytogenes.
In: Molecular Microbiology, Jg. 56 (2005-04-15), Heft 2, S. 383-396
Online
academicJournal
Zugriff:
Listeria monocytogenesis a pathogen that causes listeriosis, a severe food-borne infection. This bacterium, in order to survive and grow in the multiple conditions encountered in the host and the environment, has evolved a large number of regulatory elements, in particular many signal transduction systems based on reversible phosphorylation. The genome sequence has revealed genes for 16 putative two-component systems, four putative tyrosine phosphatases, three putative serine-threonine kinases and two putative serine-threonine phosphatases. We found that one of the latter genes,stp, encodes a functional Mn 2+ -dependent serine-threonine phosphatase similar to PPM eukaryotic phosphatases (Mg 2+ -or Mn 2+ -dependent protein phosphatase) and is required for growth ofL. monocytogenesin a murine model of infection. We identified as the first target for Stp, the elongation factor EF-Tu. Post-translational phosphorylation of EF-Tu had been shown to prevent its binding to amino-acylated transfer RNA as well as to kirromycin, an antibiotic known to inhibit EF-Tu function. Accordingly, anstpdeletion mutant is less sensitive to kirromycin. These results suggest an important role for Stp in regulating EF-Tu and controlling bacterial survival in the infected host. [ABSTRACT FROM AUTHOR]
Copyright of Molecular Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Translation elongation factor EF-Tu is a target for Stp, a serine-threonine phosphatase involved in virulence ofListeria monocytogenes.
|
---|---|
Autor/in / Beteiligte Person: | Archambaud, Cristel ; Gouin, Edith ; Pizarro-Cerda, Javier ; Cossart, Pascale ; Dussurget, Olivier |
Link: | |
Zeitschrift: | Molecular Microbiology, Jg. 56 (2005-04-15), Heft 2, S. 383-396 |
Veröffentlichung: | 2005 |
Medientyp: | academicJournal |
ISSN: | 0950-382X (print) |
DOI: | 10.1111/j.1365-2958.2005.04551.x |
Schlagwort: |
|
Sonstiges: |
|