Homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus Pleurotus ostreatus.
In: Applied Microbiology & Biotechnology, Jg. 55 (2001-05-15), Heft 5, S. 566-570
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Zugriff:
Pleurotus ostreatus is a white-rot fungus known as an efficient degrader of lignin and also various organo-pollutants. Using a DNA-mediated transformation system, a molecular breeding approach to isolate overproducers of a manganese peroxidase isozyme, MnP3, was carried out. Recombinant mnp3 constructs under the control of P. ostreatus sdi1 expression signals were introduced into the wild-type P. ostreatus strain by co-transformation with a carboxin-resistant vector plasmid, pTM1. One of the recombinants obtained by a mating between two monokaryotic transformants, TMG9-C1, showed a several times higher level of MnP activity than the wild-type control in the early stage of liquid culture. Predominant transcription of the recombinant mnp3 in the strain was demonstrated by RT-PCR experiments. This is the first report of a genetically modified P. ostreatus strain with an expression system for recombinant genes. [ABSTRACT FROM AUTHOR]
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Titel: |
Homologous expression of recombinant manganese peroxidase genes in ligninolytic fungus Pleurotus ostreatus.
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Autor/in / Beteiligte Person: | Irie, T. ; Honda, Y. ; Watanabe, T. ; Kuwahara, M. |
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Zeitschrift: | Applied Microbiology & Biotechnology, Jg. 55 (2001-05-15), Heft 5, S. 566-570 |
Veröffentlichung: | 2001 |
Medientyp: | academicJournal |
ISSN: | 0175-7598 (print) |
DOI: | 10.1007/s002530000540 |
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