Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO<subscript>2</subscript>.
In: Green Chemistry, Jg. 23 (2021-09-21), Heft 18, S. 6990-7000
Online
academicJournal
Zugriff:
The efficient bioactivation of CO 2 provides an alternative new strategy for producing high value chemicals and fuels. Normally, in vitro activation of CO 2 can be achieved by using formate dehydrogenase (FDH). However, the CO 2 solubility and the activity of commercial FDHCb remain a tough challenge for the efficient bioactivation of CO 2 . Here, we report a new "ionozyme" strategy created by using ionic liquids (ILs) as a solvent and enzyme stabilizer, resulting in a 142.3-fold increase in CO 2 conversion over FDHCb. The ionozyme (FDHPa-[CH][Pro]-[TMGH][PhO]) was first developed by combing a discovered novel FDHPa with an efficient synergistic ionic microenvironment. The remarkable performance of this ionozyme is attributed to forming key intermediates [CH][ProCOO] and [TMGH][PhOCOO], stabilizing the enzyme structure with increased solvation structure, and shortening the distance (3.9 Å) between NADH and CO 2 to favor the hydride transfer by facilitating their relative orientation and forming new hydrogen bonds at the active sites. This bioactivation of CO 2 by this specific ionozyme represents ideal starting points for the sustainable carbon cycle. [ABSTRACT FROM AUTHOR]
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Titel: |
Ionozyme: ionic liquids as solvent and stabilizer for efficient bioactivation of CO<subscript>2</subscript>.
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Autor/in / Beteiligte Person: | Ji, Xiuling ; Xue, Yaju ; Li, Zhuang ; Liu, Yanrong ; Liu, Lei ; Busk, Peter Kamp ; Lange, Lene ; Huang, Yuhong ; Zhang, Suojiang |
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Zeitschrift: | Green Chemistry, Jg. 23 (2021-09-21), Heft 18, S. 6990-7000 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
ISSN: | 1463-9262 (print) |
DOI: | 10.1039/d1gc02503a |
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