A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics.
In: Journal of Infectious Diseases, Jg. 224 (2021-07-15), Heft 2, S. 218-228
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Zugriff:
Background: Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates. Methods: We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), S1 and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results. Results: The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. S1 and RBD-based EIA results had a strong correlation with microneutralization test results. Conclusions: The data indicate a combination of SARS-CoV-2 S1 or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19. [ABSTRACT FROM AUTHOR]
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Titel: |
A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics.
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Autor/in / Beteiligte Person: | Jalkanen, Pinja ; Pasternack, Arja ; Maljanen, Sari ; Melén, Krister ; Kolehmainen, Pekka ; Huttunen, Moona ; Lundberg, Rickard ; Tripathi, Lav ; Khan, Hira ; Ritvos, Mikael A ; Naves, Rauno ; Haveri, Anu ; Österlund, Pamela ; Kuivanen, Suvi ; Jääskeläinen, Anne J ; Kurkela, Satu ; Lappalainen, Maija ; Rantasärkkä, Kaisa ; Vuorinen, Tytti ; Hytönen, Jukka |
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Zeitschrift: | Journal of Infectious Diseases, Jg. 224 (2021-07-15), Heft 2, S. 218-228 |
Veröffentlichung: | 2021 |
Medientyp: | academicJournal |
ISSN: | 0022-1899 (print) |
DOI: | 10.1093/infdis/jiab222 |
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