Reduced ex vivo susceptibility of Plasmodium falciparum after oral artemether–lumefantrine treatment in Mali.
In: Malaria Journal, Jg. 16 (2017-02-02), S. 1-6
Online
academicJournal
Zugriff:
Background: Artemisinin-based combination therapy is the recommended first-line treatment for uncomplicated falciparum malaria worldwide. However, recent studies conducted in Mali showed an increased frequency of recurrent parasitaemia following artemether–lumefantrine (AL) treatment. Methods: Study samples were collected during a large WANECAM study. Ex-vivo Plasmodium falciparum sensitivity to artemether and lumefantrine was assessed using the tritiated hypoxanthine-based assay. The prevalence of molecular markers of anti-malarial drug resistance (pfcrt K76T, pfmdr1 N86Y and K13-propeller) were measured by PCR and/or sequencing. Results: Overall 61 samples were successfully analysed in ex vivo studies. Mean IC 50 s increased significantly between baseline and recurrent parasites for both artemether (1.6 nM vs 3.2 nM, p < 0.001) and lumefantrine (1.4 nM vs 3.4 nM, p = 0.004). Wild type Pfmdr1 N86 allele was selected after treatment (71 vs 91%, 112 of 158 vs 95 of 105, p < 0.001) but not the wild type pfcrt K76 variant (23.5 vs 24.8%, 40 of 170 vs 26 of 105, p = 0.9). Three non-synonymous K13-propeller SNPs (A522C, A578S, and G638R) were found with allele frequencies <2%. Conclusion: Malian post-AL P. falciparum isolates were less susceptible to artemether and lumefantrine than baseline isolates. [ABSTRACT FROM AUTHOR]
Copyright of Malaria Journal is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Reduced ex vivo susceptibility of Plasmodium falciparum after oral artemether–lumefantrine treatment in Mali.
|
---|---|
Autor/in / Beteiligte Person: | Dama, Souleymane ; Niangaly, Hamidou ; Ouattara, Amed ; Sagara, Issaka ; Sissoko, Sekou ; Traore, Oumar Bila ; Bamadio, Amadou ; Dara, Niawanlou ; Djimde, Moussa ; Alhousseini, Mohamed Lamine ; Goita, Siaka ; Maiga, Hamma ; Dara, Antoine ; Doumbo, Ogobara K. ; Djimde, Abdoulaye A. |
Link: | |
Zeitschrift: | Malaria Journal, Jg. 16 (2017-02-02), S. 1-6 |
Veröffentlichung: | 2017 |
Medientyp: | academicJournal |
ISSN: | 1475-2875 (print) |
DOI: | 10.1186/s12936-017-1700-8 |
Schlagwort: |
|
Sonstiges: |
|