Assembly of an Evolutionarily Conserved Alternative Proteasome Isoform in Human Cells.
In: Cell Reports, Jg. 14 (2016-03-29), Heft 12, S. 2962-2974
academicJournal
Zugriff:
Summary Targeted intracellular protein degradation in eukaryotes is largely mediated by the proteasome. Here, we report the formation of an alternative proteasome isoform in human cells, previously found only in budding yeast, that bears an altered subunit arrangement in the outer ring of the proteasome core particle. These proteasomes result from incorporation of an additional α4 (PSMA7) subunit in the position normally occupied by α3 (PSMA4). Assembly of “α4-α4” proteasomes depends on the relative cellular levels of α4 and α3 and on the proteasome assembly chaperone PAC3. The oncogenic tyrosine kinases ABL and ARG and the tumor suppressor BRCA1 regulate cellular α4 levels and formation of α4-α4 proteasomes. Cells primed to assemble α4-α4 proteasomes exhibit enhanced resistance to toxic metal ions. Taken together, our results establish the existence of an alternative mammalian proteasome isoform and suggest a potential role in enabling cells to adapt to environmental stresses. [ABSTRACT FROM AUTHOR]
Copyright of Cell Reports is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Assembly of an Evolutionarily Conserved Alternative Proteasome Isoform in Human Cells.
|
---|---|
Autor/in / Beteiligte Person: | Padmanabhan, Achuth ; Vuong, Simone Anh-Thu ; Hochstrasser, Mark |
Zeitschrift: | Cell Reports, Jg. 14 (2016-03-29), Heft 12, S. 2962-2974 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 2639-1856 (print) |
DOI: | 10.1016/j.celrep.2016.02.068 |
Sonstiges: |
|