Tomato thymidine kinase-based suicide gene therapy for malignant glioma-an alternative for Herpes Simplex virus-1 thymidine kinase.
In: Cancer Gene Therapy, Jg. 22 (2015-03-01), Heft 3, S. 130-137
Online
academicJournal
Zugriff:
Malignant gliomas (MGs) are the most common malignant primary brain tumors with a short life estimate accompanied by a marked reduction in the quality of life. Herpes Simplex virus-1 thymidine kinase ganciclovir (HSV-TK/GCV) system is the best characterized enzyme prodrug therapy in use. However, lipophobicity of GCV and low enzymatic activity of HSV-TK reduce the treatment efficacy. Tomato TK (ToTK) has shown high activity in combination with its specific substrate azidothymidine (AZT). The aim of this study was to evaluate whether ToTK/AZT could be used as an alternative to HSV-TK/GCV therapy. Both treatments demonstrated cytotoxicity in human MG cells in vitro. In vivo, both treatments decreased tumor growth and tumors were smaller in comparison with controls in mouse orthotopic MG model. Survival of ToTK/AZT-treated mice was significantly increased compared with control mice (*P<0.05) but not as compared with HSV-TK/GCV-treated mice. No significant differences were observed in clinical chemistry safety analyses. We conclude that both treatments showed a beneficial treatment response in comparison to controls on tumor growth and ToTK/AZT also on survival. There were no significant differences between these treatments. Therefore ToTK/AZT could be considered as an alternative treatment option for MG because of its favorable therapeutic characteristics. [ABSTRACT FROM AUTHOR]
Copyright of Cancer Gene Therapy is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Tomato thymidine kinase-based suicide gene therapy for malignant glioma-an alternative for Herpes Simplex virus-1 thymidine kinase.
|
---|---|
Autor/in / Beteiligte Person: | Stedt, H ; Samaranayake, H ; Kurkipuro, J ; Wirth, G ; Christiansen, L S ; Vuorio, T ; Määttä, A-M ; Piškur, J ; Ylä-Herttuala, S |
Link: | |
Zeitschrift: | Cancer Gene Therapy, Jg. 22 (2015-03-01), Heft 3, S. 130-137 |
Veröffentlichung: | 2015 |
Medientyp: | academicJournal |
ISSN: | 0929-1903 (print) |
DOI: | 10.1038/cgt.2014.76 |
Schlagwort: |
|
Sonstiges: |
|