Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla <subscript>KPC-2</subscript> and bla <subscript>NDM-1</subscript> co-carrying hypertransmissible IncM1 plasmid.
In: Communications biology, Jg. 7 (2024-06-06), Heft 1, S. 695
Online
academicJournal
Zugriff:
Infection caused by KPC and NDM carbapenemases co-producing Klebsiella pneumoniae (KPC_NDM_CRKP) poses serious public health concerns. Here, we elucidate the prevalence of a hypertransmissible lncM1 plasmid, pKPC_NDM, co-carrying bla KPC-2 and bla NDM-1 genes in sequence type 1049 K_locus 5 (ST1049-KL5) KPC_NDM_CRKP isolates. Genetic and clonal relatedness analyses using pulsed-field gel electrophoresis, single nucleotide polymorphism analysis and core genome multilocus sequence typing suggested clonal dissemination of ST1049-KL5 KPC_NDM_CRKP strains in our hospital. Whole genome sequencing identified an identical 76,517 bp- bla KPC-2 and bla NDM-1 genes co-carrying IncM1 plasmid pKPC_NDM and a pLVPK-like hypervirulent plasmid in all ST1049-KL5 KPC_NDM_CRKP isolates. pKPC_NDM shared 100% identity with a previously sequenced plasmid CRKP35_unnamed4, demonstrating high transferability in conjugation assay, with conjugation frequencies reaching 10 -4 and 10 -5 in Escherichia coli and K. pneumoniae recipients, respectively. It also maintained favorable stability and flexible compatibility, with retention rates exceeding 80% after 10 days of continuous passage, and could be compatible with pre-existing bla KPC - or bla NDM -carrying plasmids in recipient strains. This study summarizes the characteristics of KPC_NDM_CRKP outbreaks and highlights the importance of ongoing surveillance and infection control strategies to address the challenges posed by ST1049 K. pneumoniae strains.
(© 2024. The Author(s).)
Titel: |
Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla <subscript>KPC-2</subscript> and bla <subscript>NDM-1</subscript> co-carrying hypertransmissible IncM1 plasmid.
|
---|---|
Autor/in / Beteiligte Person: | Liu, H ; Xiang, Y ; Xiong, M ; Xiao, X ; Zhou, J ; Tian, H ; Chen, Q ; Li, Y |
Link: | |
Zeitschrift: | Communications biology, Jg. 7 (2024-06-06), Heft 1, S. 695 |
Veröffentlichung: | London, United Kingdom : Nature Publishing Group UK, [2018]-, 2024 |
Medientyp: | academicJournal |
ISSN: | 2399-3642 (electronic) |
DOI: | 10.1038/s42003-024-06398-w |
Schlagwort: |
|
Sonstiges: |
|