Pre-pandemic antibodies screening against SARS-CoV-2 and virus detection among children diagnosed with eruptive fevers.
In: International journal of immunopathology and pharmacology, Jg. 38 (2024), S. 3946320241260633
Online
academicJournal
Zugriff:
Objectives: This study aims to assess the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibodies against the spike (S) and nucleocapsid (NP) proteins, as well as neutralizing antibodies against the receptor-binding domain (RBD). Additionally, it aims to detect viral RNA of SARS-CoV-2 in pre-pandemic archival pediatric specimens collected before the announcement of the COVID-19 pandemic spread on March 20 th , 2020, in Morocco. The objective is to investigate the existence of pre-pandemic immunity to SARS-CoV-2.
Methods: We conducted a cross-sectional study, to analyze IgG antibody levels in a cohort of 106 pre-pandemic pediatric participants. Using an indirect enzyme-linked immunosorbent assay (ELISA), we measured the IgG levels against the S and NP proteins of SARS-CoV-2. Additionally, we staged a competitive ELISA assay to evaluate the neutralizing capability of these antibodies. We used reverse transcription polymerase chain reaction (rRT-PCR) to detect viral NP and ORF1ab genes of SARS-CoV-2 in oropharyngeal swabs. Moreover, we conducted on the same specimens a multiplexed RT-PCR to detect RNA of the most common 27 pathogens involved in lower respiratory tract infections.
Results: Among the 106 serum samples, 13% ( n n = =14) tested positive for SARS-CoV-2 IgG antibodies using ELISA. Temporal analysis indicated varying IgG positivity levels across 2019. Neutralizing antibodies were found in 21% of the 28 samples analyzed, including two with high inhibition rates (93%). The SARS-CoV-2 RNA was detected using rRT-PCR in 14 samples. None of the samples tested positive for the other 27 pathogens associated with lower respiratory tract infections, using multiplexed RT-PCR.
Conclusion: Our study addresses the possibility, that COVID-19 infections occurred in Morocco before the recognized outbreak. On the other hand, some of the cases might reflect cross-reactivity with other coronaviruses or be influenced by previous viral exposures or vaccinations. Understanding these factors is crucial to comprehending pediatric immune responses to newly emerging infectious diseases.
Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Titel: |
Pre-pandemic antibodies screening against SARS-CoV-2 and virus detection among children diagnosed with eruptive fevers.
|
---|---|
Autor/in / Beteiligte Person: | Najimi, N ; Tajount, L ; Regragui, Z ; Remz, C ; Ait-Lhaj-Mhand, R ; Kadi, C ; Belayachi, L ; Seghrouchni, F ; Dakka, Nadia ; El Hassani, RA ; Elharti, E ; Oumzil, H ; Bakri, Y |
Link: | |
Zeitschrift: | International journal of immunopathology and pharmacology, Jg. 38 (2024), S. 3946320241260633 |
Veröffentlichung: | 2015- : London : Sage Publications ; <i>Original Publication</i>: [Chieti, Italy?] : Biomedical Research Press, 2024 |
Medientyp: | academicJournal |
ISSN: | 2058-7384 (electronic) |
DOI: | 10.1177/03946320241260633 |
Schlagwort: |
|
Sonstiges: |
|