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Daily monitoring of serum uric acid levels is very important to provide appropriate treatment according to the constitution and lifestyle of individual hyperuricemic patients. We have developed a suspension-based assay to measure uric acid by adding a sample solution to the suspension containing micro-sized particles immobilized on uricase and horseradish peroxidase (HRP). In the proposed method, the mediator reaction of uricase, HRP, and uric acid produces resorufin from Amplex red. This resorufin is adsorbed onto enzyme-immobilized micro-sized particles simultaneously with its production, resulting in the red color of the micro-sized particles. The concentration of resorufin on the small surface area of the microscopic particles achieves a colorimetric analysis of uric acid with superior visibility. In addition, ethanol-induced desorption of resorufin allowed quantitative measurement of uric acid using a 96-well fluorescent microplate reader. The limit of detection (3σ) and RSD (n = 3) were estimated to be 2.2 × 10 -2 μg/mL and ≤ 12.1%, respectively. This approach could also be applied to a portable fluorometer.

Titel:	Colorimetric and fluorometric determination of uric acid by a suspension-based assay using enzyme-immobilized micro-sized particles.
Autor/in / Beteiligte Person:	Moriiwa, Y ; Hatakeyama, K ; Morioka, K ; Inoue, Y ; Murakami, H ; Teshima, N ; Yanagida, A ; Shoji, A
Link:	Zum Volltext
Zeitschrift:	Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Jg. 40 (2024-05-01), Heft 5, S. 951
Veröffentlichung:	2022- : [Cham] : Springer ; <i>Original Publication</i>: Tokyo : The Society, [1985-, 2024
Medientyp:	academicJournal
ISSN:	1348-2246 (electronic)
DOI:	10.1007/s44211-024-00556-0
Schlagwort:	Particle Size Humans Suspensions Oxazines chemistry Uric Acid blood Uric Acid chemistry Uric Acid analysis Colorimetry Enzymes, Immobilized chemistry Enzymes, Immobilized metabolism Urate Oxidase chemistry Urate Oxidase metabolism Horseradish Peroxidase chemistry Horseradish Peroxidase metabolism Fluorometry
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Anal Sci] 2024 May; Vol. 40 (5), pp. 951-958. <i>Date of Electronic Publication: </i>2024 Apr 10. MeSH Terms: Uric Acid* / blood ; Uric Acid* / chemistry ; Uric Acid* / analysis ; Colorimetry* ; Enzymes, Immobilized* / chemistry ; Enzymes, Immobilized* / metabolism ; Urate Oxidase* / chemistry ; Urate Oxidase* / metabolism ; Horseradish Peroxidase* / chemistry ; Horseradish Peroxidase* / metabolism ; Fluorometry* ; Particle Size ; Humans ; Suspensions ; Oxazines / chemistry References: T. Petreski, R. Ekart, R. Hojs, S. Bevc, Hyperuricemia, the heart, and the kidneys – to treat or not to treat? Ren. Fail. 42, 978–986 (2020). (PMID: 10.1080/0886022X.2020.1822185329722847534372) ; Y. Saito, A. Tanaka, K. Node, Y. Kobayashi, Uric acid and cardiovascular disease: a clinical review. J. Cardiol. 78, 51–57 (2021). 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Amine, A highly sensitive colorimetric DNA sensor for MicroRNA-155 detection: leveraging the peroxidase-like activity of copper nanoparticles in a double amplification strategy. Mikrochim. Acta 191, 32 (2024). (PMID: 10.1007/s00604-023-06087-1) Grant Information: 23H03865 Japan Society for the Promotion of Science; 23K14337 Gesellschaft zur Förderung Kynologischer Forschung Contributed Indexing: Keywords: Colorimetric assay; Mediator reaction; Micro-sized particles; Suspension-based assay; Ureic acid Substance Nomenclature: 268B43MJ25 (Uric Acid) ; 0 (Enzymes, Immobilized) ; EC 1.7.3.3 (Urate Oxidase) ; EC 1.11.1.- (Horseradish Peroxidase) ; 635-78-9 (resorufin) ; 0 (Suspensions) ; 0 (Oxazines) Entry Date(s): Date Created: 20240410 Date Completed: 20240424 Latest Revision: 20240424 Update Code: 20240425

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Colorimetric and fluorometric determination of uric acid by a suspension-based assay using enzyme-immobilized micro-sized particles.