Icariin mitigates anxiety-like behaviors induced by hemorrhagic shock and resuscitation via inhibiting of astrocytic activation.

Zhang, DX ; Jia, SY ; et al.

In: Phytomedicine : international journal of phytotherapy and phytopharmacology, Jg. 128 (2024-06-01), S. 155507

Online academicJournal

Zugriff:

Zum Volltext

Background: Abnormal activation of astrocytes in the amygdala contributes to anxiety after hemorrhagic shock and resuscitation (HSR). Nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-associated epigenetic reprogramming of astrocytic activation is crucial to anxiety. A bioactive monomer derived from Epimedium icariin (ICA) has been reported to modulate NF-κB signaling and astrocytic activation.

Purpose: The present study aimed to investigate the effects of ICA on post-HSR anxiety disorders and its potential mechanism of action.

Methods: We first induced HSR in mice through a bleeding and re-transfusion model and selectively inhibited and activated astrocytes in the amygdala using chemogenetics. Then, ICA (40 mg/kg) was administered by oral gavage once daily for 21 days. Behavioral, electrophysiological, and pathological changes were assessed after HSR using the light-dark transition test, elevated plus maze, recording of local field potential (LFP), and immunofluorescence assays.

Results: Exposure to HSR reduced the duration of the light chamber and attenuated open-arm entries. Moreover, HSR exposure increased the theta oscillation power in the amygdala and upregulated NF-κB p65, H3K27ac, and H3K4me3 expression. Contrarily, chemogenetic inhibition of astrocytes significantly reversed these changes. Chemogenetic inhibition in astrocytes was simulated by ICA, but chemogenetic activation of astrocytes blocked the neuroprotective effects of ICA.

Conclusion: ICA mitigated anxiety-like behaviors induced by HSR in mice via inhibiting astrocytic activation, which is possibly associated with NF-κB-induced epigenetic reprogramming.

Competing Interests: Declaration of competing interest All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest, or non-financial competing interest in the subject matter or materials discussed in this manuscript by any of the authors.

Titel:	Icariin mitigates anxiety-like behaviors induced by hemorrhagic shock and resuscitation via inhibiting of astrocytic activation.
Autor/in / Beteiligte Person:	Zhang, DX ; Jia, SY ; Xiao, K ; Zhang, MM ; Yu, ZF ; Liu, JZ ; Zhang, W ; Zhang, LM ; Xing, BR ; Zhou, TT ; Li, XM ; Zhao, XC ; An, P
Link:	Zum Volltext
Zeitschrift:	Phytomedicine : international journal of phytotherapy and phytopharmacology, Jg. 128 (2024-06-01), S. 155507
Veröffentlichung:	Stuttgart : Urban & Fischer Verlag ; <i>Original Publication</i>: Stuttgart ; New York : G. Fischer, c1994-, 2024
Medientyp:	academicJournal
ISSN:	1618-095X (electronic)
DOI:	10.1016/j.phymed.2024.155507
Schlagwort:	Animals Mice Male Resuscitation methods Disease Models, Animal Mice, Inbred C57BL NF-kappa B metabolism Behavior, Animal drug effects Amygdala drug effects Epimedium chemistry Astrocytes drug effects Flavonoids pharmacology Shock, Hemorrhagic drug therapy Anxiety drug therapy
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Phytomedicine] 2024 Jun; Vol. 128, pp. 155507. <i>Date of Electronic Publication: </i>2024 Mar 19. MeSH Terms: Astrocytes* / drug effects ; Flavonoids* / pharmacology ; Shock, Hemorrhagic* / drug therapy ; Anxiety* / drug therapy ; Animals ; Mice ; Male ; Resuscitation / methods ; Disease Models, Animal ; Mice, Inbred C57BL ; NF-kappa B / metabolism ; Behavior, Animal / drug effects ; Amygdala / drug effects ; Epimedium / chemistry Contributed Indexing: Keywords: Amygdala; Anxiety; Astrocytes; Epigenetic reprogramming; Hemorrhagic shock and resuscitation Substance Nomenclature: VNM47R2QSQ (icariin) ; 0 (Flavonoids) ; 0 (NF-kappa B) Entry Date(s): Date Created: 20240329 Date Completed: 20240430 Latest Revision: 20240430 Update Code: 20240501

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.