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Human umbilical cord mesenchymal stem cells (hUMSCs) belong to a multipotent stem cell population. Transplantation of icariin (ICA)-treated hUMSCs have better tissue repairing function in chronic liver injury. This study was to investigate whether the tissue-repairing effects and migration of hUMSCs after ICA treatment were regulated by circular RNAs (circRNAs). ICA was used to treat hUMSCs in vitro for 1 week and the expression profiles of circRNAs were generated using RNA sequencing. Differentially expressed circRNAs in hUMSCs after ICA intervention were screened. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis were carried out to predict the potential function of dysregulated circRNAs. There were 52 differentially expressed circRNAs (32 circRNAs up-regulated and 20 circRNAs down-regulated) with fold change ≥2.0 before and after ICA treatment. ADP-ribosylation factors were associated with the dysregulated circRNAs among Gene Ontology analysis. Kyoto Encyclopedia of Genes and Genomes analysis showed that only endocytosis pathway was associated with up-regulated circRNAs, whereas 4 pathways including homologous recombination, RNA transport, axon guidance, and proteoglycans in cancer were related to down-regulated circRNAs. Fifty-two differentially expressed circRNAs and 238 predicted microRNAs were included in circRNAs-microRNAs network. The mechanism of ICA inducing hUMSCs migration may be through regulating circRNAs expression which affects ADP-ribosylation factors protein signal pathways.

Competing Interests: The authors have no conflicts of interest to disclose.

Titel:	Differential expression of circular RNAs in human umbilical cord mesenchymal stem cells treated with icariin.
Autor/in / Beteiligte Person:	Liu, X ; Chu, X ; Li, L ; Man, S ; Wang, L ; Bian, Y ; Zhou, H
Link:	Zum Volltext
Zeitschrift:	Medicine, Jg. 103 (2024-03-22), Heft 12, S. e37549
Veröffentlichung:	Hagerstown, Md : Lippincott Williams & Wilkins, 2024
Medientyp:	academicJournal
ISSN:	1536-5964 (electronic)
DOI:	10.1097/MD.0000000000037549
Schlagwort:	Humans RNA, Circular genetics Umbilical Cord ADP-Ribosylation Factors genetics Gene Expression Profiling MicroRNAs genetics Mesenchymal Stem Cells Flavonoids
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Medicine (Baltimore)] 2024 Mar 22; Vol. 103 (12), pp. e37549. MeSH Terms: MicroRNAs* / genetics ; Mesenchymal Stem Cells* ; Flavonoids* ; Humans ; RNA, Circular / genetics ; Umbilical Cord ; ADP-Ribosylation Factors / genetics ; Gene Expression Profiling References: Feng H, Liu Q, Deng Z, et al. Human umbilical cord mesenchymal stem cells ameliorate erectile dysfunction in rats with diabetes mellitus through the attenuation of ferroptosis. Stem Cell Res Ther. 2022;13:450. ; Shaikh MS, Shahzad Z, Tash EA, et al. Human umbilical cord mesenchymal stem cells: current literature and role in periodontal regeneration. Cells. 2022;11:1168. ; He Y, Guo X, Lan T, et al. Human umbilical cord-derived mesenchymal stem cells improve the function of liver in rats with acute-on-chronic liver failure via downregulating Notch and Stat1/Stat3 signaling. 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Nat Commun. 2016;7:11215. Substance Nomenclature: 0 (RNA, Circular) ; VNM47R2QSQ (icariin) ; 0 (MicroRNAs) ; EC 3.6.5.2 (ADP-Ribosylation Factors) ; 0 (Flavonoids) Entry Date(s): Date Created: 20240322 Date Completed: 20240325 Latest Revision: 20240329 Update Code: 20240330 PubMed Central ID: PMC10956971

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Differential expression of circular RNAs in human umbilical cord mesenchymal stem cells treated with icariin.