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Background: Research has demonstrated the anti-photoaging properties of glabridin and bakuchiol.

Methods: The impact of glabridin, glabridin + bakuchiol, and bakuchiol on the levels of tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) in mice skin fibroblasts was observed. Furthermore, we investigated the potential roles of fibronectin (FN), interferon-γ (IFN-γ), interleukin-22 (IL-22), and transforming growth factor-β (TGF-β) in the tissues, and evaluated their impact on the enzymatic levels in the skin. In conjunction with transcriptomic analysis, metabolomic profiling, and network pharmacology, all samples underwent comprehensive metabolomic and principal component analysis. The Venny2.1 method was utilized to identify variances in shared metabolites between the treatment group and the UVB group, as well as between the UVB group and the control group. Subsequently, a cluster heat map was generated to forecast and analyze metabolic pathways and targets.

Results: The outcomes from the hematoxylin and eosin and toluidine blue staining revealed that glabridin and bakuchiol markedly decreased dermal thickness and suppressed mast cell infiltration in photoaged mice. Immunohistochemistry and Elisa analysis revealed that glabridin and bakuchiol effectively attenuated the levels of pro-inflammatory factors, including IL-1β, tumor necrosis factor-α, IL-22, and IFN-γ. Furthermore, an increase in the levels of anti-inflammatory factors such as FN and TGF-β was also observed. The determination of the contents of superoxide dismutase, hydroxypropyltransferase and malondialdehyde in mice dorsal skin revealed that glabridin and bakuchiol not only elevated the levels of superoxide dismutase and hydroxyproline, but also reduced malondialdehyde content. Due to the limited number of shared differential metabolites exclusively within Kyoto Encyclopedia of Genes and Genomes, comprehensive pathway enrichment analysis was not feasible.

Conclusion: This study demonstrates that glabridin and bakuchiol effectively impede photoaging and alleviate skin inflammation in mice.

Titel:	Effect of glabridin combined with bakuchiol on UVB-induced skin damage and its underlying mechanism: An experimental study.
Autor/in / Beteiligte Person:	Peng, G ; Li, Y ; Zeng, Y ; Sun, B ; Zhang, L ; Liu, Q
Link:	Zum Volltext
Zeitschrift:	Journal of cosmetic dermatology, Jg. 23 (2024-06-01), Heft 6, S. 2256-2269
Veröffentlichung:	Oxford, UK : Blackwell Science, c2002-, 2024
Medientyp:	academicJournal
ISSN:	1473-2165 (electronic)
DOI:	10.1111/jocd.16259
Schlagwort:	Animals Mice Fibroblasts drug effects Fibroblasts metabolism Fibroblasts radiation effects Interleukin-1beta metabolism Tumor Necrosis Factor-alpha metabolism Interleukins metabolism Fibronectins metabolism Interleukin-22 Female Interferon-gamma metabolism Transforming Growth Factor beta metabolism Phenols pharmacology Skin Aging drug effects Skin Aging radiation effects Ultraviolet Rays adverse effects Isoflavones pharmacology Skin drug effects Skin radiation effects Skin pathology Skin metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Cosmet Dermatol] 2024 Jun; Vol. 23 (6), pp. 2256-2269. <i>Date of Electronic Publication: </i>2024 Mar 18. MeSH Terms: Phenols* / pharmacology ; Skin Aging* / drug effects ; Skin Aging* / radiation effects ; Ultraviolet Rays* / adverse effects ; Isoflavones* / pharmacology ; Skin* / drug effects ; Skin* / radiation effects ; Skin* / pathology ; Skin* / metabolism ; Animals ; Mice ; Fibroblasts / drug effects ; Fibroblasts / metabolism ; Fibroblasts / radiation effects ; Interleukin-1beta / metabolism ; Tumor Necrosis Factor-alpha / metabolism ; Interleukins / metabolism ; Fibronectins / metabolism ; Interleukin-22 ; Female ; Interferon-gamma / metabolism ; Transforming Growth Factor beta / metabolism References: Parisi M, Verrillo M, Luciano MA, et al. Use of natural agents and agrifood wastes for the treatment of skin photoaging. 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Effect of glabridin combined with bakuchiol on UVB-induced skin damage and its underlying mechanism: An experimental study.