Structural insights into the octamerization of glycerol dehydrogenase.
In: PloS one, Jg. 19 (2024-03-14), Heft 3, S. e0300541
Online
academicJournal
Zugriff:
Glycerol dehydrogenase (GDH) catalyzes glycerol oxidation to dihydroxyacetone in a NAD+-dependent manner. As an initiator of the oxidative pathway of glycerol metabolism, a variety of functional and structural studies of GDH have been conducted previously. Structural studies revealed intriguing features of GDH, like the flexible β-hairpin and its significance. Another commonly reported structural feature is the enzyme's octameric oligomerization, though its structural details and functional significance remained unclear. Here, with a newly reported GDH structure, complexed with both NAD+ and glycerol, we analyzed the octamerization of GDH. Structural analyses revealed that octamerization reduces the structural dynamics of the N-domain, which contributes to more consistently maintaining a distance required for catalysis between the cofactor and substrate. This suggests that octamerization may play a key role in increasing the likelihood of the enzyme reaction by maintaining the ligands in an appropriate configuration for catalysis. These findings expand our understanding of the structure of GDH and its relation to the enzyme's activity.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Park et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Titel: |
Structural insights into the octamerization of glycerol dehydrogenase.
|
---|---|
Autor/in / Beteiligte Person: | Park, T ; Kang, JY ; Jin, M ; Yang, J ; Kim, H ; Noh, C ; Jung, CH ; Eom, SH |
Link: | |
Zeitschrift: | PloS one, Jg. 19 (2024-03-14), Heft 3, S. e0300541 |
Veröffentlichung: | San Francisco, CA : Public Library of Science, 2024 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 (electronic) |
DOI: | 10.1371/journal.pone.0300541 |
Schlagwort: |
|
Sonstiges: |
|