Einzeltreffer — DigiBib

Vibrio parahaemolyticus is the primary foodborne pathogen known to cause gastrointestinal infections in humans. Nevertheless, the molecular mechanisms of V. parahaemolyticus pathogenicity are not fully understood. Prophages carry virulence and antibiotic resistance genes commonly found in Vibrio populations, and they facilitate the spread of virulence and the emergence of pathogenic Vibrio strains. In this study, we characterized three such genes, VpaChn25_0713 , VpaChn25_0714 , and VpaChn25_RS25055 , within the largest prophage gene cluster in V. parahaemolyticus CHN25. The deletion mutants Δ VpaChn25_RS25055 , Δ VpaChn25_0713 , Δ VpaChn25_0714 , and Δ VpaChn25_RS25055-0713-0714 were derived with homologous recombination, and the complementary mutants Δ VpaChn25_0713 -com, Δ VpaChn25_0714 -com, Δ VpaChn25_RS25055 -com, Δ VpaChn25_RS25055-0713-0714 -com were also constructed. In the absence of the VpaChn25_RS25055 , VpaChn25_0713 , VpaChn25_0714 , and VpaChn25_RS25055-0713-0714 genes, the mutants showed significant reductions in low-temperature survivability and biofilm formation ( p < 0.001). The Δ VpaChn25_0713 , Δ VpaChn25_RS25055 , and Δ VpaChn25_RS25055-0713-0714 mutants were also significantly defective in swimming motility ( p < 0.001). In the Caco-2 model, the above four mutants attenuated the cytotoxic effects of V. parahaemolyticus CHN25 on human intestinal epithelial cells ( p < 0.01), especially the Δ VpaChn25_RS25055 and Δ VpaChn25_RS25055-0713-0714 mutants. Transcriptomic analysis showed that 15, 14, 8, and 11 metabolic pathways were changed in the Δ VpaChn25_RS25055 , Δ VpaChn25_0713 , Δ VpaChn25_0714 , and Δ VpaChn25_RS25055-0713-0714 mutants, respectively. We labeled the VpaChn25_RS25055 gene with superfolder green fluorescent protein (sfGFP) and found it localized at both poles of the bacteria cell. In addition, we analyzed the evolutionary origins of the above genes. In summary, the prophage genes VpaChn25_0713 , VpaChn25_0714 , and VpaChn25_RS25055 enhance V. parahaemolyticus CHN25's survival in the environment and host. Our work improves the comprehension of the synergy between prophage-associated genes and the evolutionary process of V. parahaemolyticus .

Titel:	Biological Function of Prophage-Related Gene Cluster Δ VpaChn25_RS25055 ~Δ VpaChn25_0714 of Vibrio parahaemolyticus CHN25.
Autor/in / Beteiligte Person:	Zhao, H ; Xu, Y ; Yang, L ; Wang, Y ; Li, M ; Chen, L
Link:	Zum Volltext
Zeitschrift:	International journal of molecular sciences, Jg. 25 (2024-01-23), Heft 3
Veröffentlichung:	Basel, Switzerland : MDPI, [2000-, 2024
Medientyp:	academicJournal
ISSN:	1422-0067 (electronic)
DOI:	10.3390/ijms25031393
Schlagwort:	Humans Prophages genetics Caco-2 Cells Virulence genetics Multigene Family Vibrio parahaemolyticus metabolism Vibrio Infections microbiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Int J Mol Sci] 2024 Jan 23; Vol. 25 (3). <i>Date of Electronic Publication: </i>2024 Jan 23. MeSH Terms: Vibrio parahaemolyticus* / metabolism ; Vibrio Infections* / microbiology ; Humans ; Prophages / genetics ; Caco-2 Cells ; Virulence / genetics ; Multigene Family References: J Mol Microbiol Biotechnol. 2015;25(2-3):73-8. (PMID: 26159069) ; Semin Cell Dev Biol. 2023 Feb 28;136:64-74. (PMID: 35410715) ; Front Cell Infect Microbiol. 2020 Dec 09;10:595709. (PMID: 33363055) ; Amino Acids. 2019 Aug;51(8):1103-1127. (PMID: 31267155) ; J Appl Microbiol. 2008 May;104(5):1283-93. (PMID: 18031521) ; Appl Environ Microbiol. 2021 Oct 14;87(21):e0152721. (PMID: 34406825) ; J Bacteriol. 2017 Nov 14;199(24):. (PMID: 28947672) ; Int J Mol Sci. 2022 Jun 25;23(13):. (PMID: 35806089) ; J Bacteriol. 1985 Apr;162(1):98-101. (PMID: 3884601) ; Eur J Biochem. 2000 Jun;267(12):3640-6. (PMID: 10848981) ; Microbiol Mol Biol Rev. 2001 Sep;65(3):445-62, table of contents. (PMID: 11528005) ; Mar Pollut Bull. 2020 Nov;160:111554. (PMID: 32810672) ; Curr Genet. 2022 Apr;68(2):267-287. (PMID: 35064802) ; Sci Rep. 2017 Jan 20;7:40878. (PMID: 28106112) ; Bio Protoc. 2021 Dec 20;11(24):e4264. (PMID: 35087923) ; Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):19487-19496. (PMID: 32723820) ; Virulence. 2018 Jan 1;9(1):22-24. (PMID: 28981394) ; Appl Environ Microbiol. 2009 Mar;75(6):1697-702. (PMID: 19151181) ; Front Microbiol. 2023 Jun 28;14:1171350. (PMID: 37448578) ; Cell Host Microbe. 2021 Nov 10;29(11):1620-1633.e8. (PMID: 34597593) ; FEBS J. 2023 Feb;290(3):584-599. (PMID: 34822226) ; Arch Virol. 2022 Feb;167(2):531-544. (PMID: 35024965) ; Sci Rep. 2017 Mar 30;7:45504. (PMID: 28358054) ; Sci Rep. 2017 Dec 7;7(1):17183. (PMID: 29215019) ; Molecules. 2018 May 17;23(5):. (PMID: 29772798) ; Nucleic Acids Res. 2010 May;38(8):2624-36. (PMID: 20385581) ; Rev Chilena Infectol. 2005 Jun;22(2):125-30. (PMID: 15891792) ; Curr Opin Cell Biol. 2011 Feb;23(1):71-7. (PMID: 21095111) ; Cold Spring Harb Perspect Biol. 2021 Jan 4;13(1):. (PMID: 33397651) ; Front Microbiol. 2020 Jul 14;11:1453. (PMID: 32765437) ; Sci Rep. 2017 Aug 1;7(1):6990. (PMID: 28765554) ; BMC Genomics. 2017 Jun 5;18(1):436. (PMID: 28583064) ; J Bacteriol. 2005 Jun;187(11):3752-61. (PMID: 15901699) ; Biochem Soc Trans. 2015 Oct;43(5):1033-40. (PMID: 26517919) ; Front Microbiol. 2015 Jul 17;6:707. (PMID: 26236293) ; Microbiol Spectr. 2022 Aug 31;10(4):e0072122. (PMID: 35938859) ; Front Microbiol. 2021 Mar 17;12:655239. (PMID: 33815347) ; Appl Environ Microbiol. 2015 Aug;81(15):5223-34. (PMID: 26025891) ; Front Nutr. 2021 Aug 20;8:663584. (PMID: 34490318) ; Genome Announc. 2013 Jan;1(1):. (PMID: 23469330) ; PLoS One. 2017 Apr 24;12(4):e0174704. (PMID: 28437463) ; Mol Genet Genomics. 2019 Aug;294(4):1007-1022. (PMID: 30968246) ; Microbiol Mol Biol Rev. 2014 Jun;78(2):231-56. (PMID: 24847021) ; Int J Mol Sci. 2021 Mar 24;22(7):. (PMID: 33805191) ; J Food Prot. 2022 Sep 1;85(9):1320-1328. (PMID: 35749698) ; Pathogens. 2022 Apr 10;11(4):. (PMID: 35456128) ; J Biol Chem. 1991 Mar 5;266(7):4119-25. (PMID: 1999405) ; J Mol Biol. 1998 Feb 27;276(3):591-602. (PMID: 9551099) ; Front Microbiol. 2017 Jun 08;8:1043. (PMID: 28642752) ; J Microbiol Methods. 2021 Jun;185:106234. (PMID: 33971217) ; Int J Mol Sci. 2014 Dec 05;15(12):22539-62. (PMID: 25490137) ; J Integr Plant Biol. 2017 Sep;59(9):642-656. (PMID: 28294536) ; Int J Mol Sci. 2022 Oct 15;23(20):. (PMID: 36293218) ; Genetics. 2017 Dec;207(4):1231-1253. (PMID: 29203701) ; Front Microbiol. 2021 Dec 13;12:785634. (PMID: 34966370) ; J Bacteriol. 2021 Sep 23;203(20):e0027621. (PMID: 34339299) ; Sci Rep. 2019 Jul 31;9(1):11081. (PMID: 31366978) ; Acta Crystallogr F Struct Biol Commun. 2020 Sep 1;76(Pt 9):406-413. (PMID: 32880588) ; J Bacteriol. 2001 Jan;183(2):680-6. (PMID: 11133963) ; Front Immunol. 2021 Oct 20;12:693932. (PMID: 34745085) ; J Bacteriol. 2016 Aug 25;198(18):2494-502. (PMID: 27381917) ; Virulence. 2013 Jul 1;4(5):354-65. (PMID: 23611873) ; Int J Biol Macromol. 2020 Mar 1;146:716-724. (PMID: 31843618) ; Front Microbiol. 2023 Oct 26;14:1294177. (PMID: 37954246) ; Mol Microbiol. 2005 Aug;57(4):1074-85. (PMID: 16091045) ; Front Microbiol. 2018 Aug 14;9:1881. (PMID: 30154782) ; Biophys J. 2017 Apr 11;112(7):1462-1471. (PMID: 28402888) ; Front Microbiol. 2021 Oct 07;12:737299. (PMID: 34690978) ; J Bacteriol. 2019 Apr 9;201(9):. (PMID: 30782635) ; Mol Microbiol. 2003 Jul;49(2):277-300. (PMID: 12886937) ; Mol Microbiol. 2007 Nov;66(4):829-39. (PMID: 17919287) ; J Microbiol Biotechnol. 2020 Nov 28;30(11):1729-1738. (PMID: 32830190) ; mBio. 2021 Mar 23;12(2):. (PMID: 33758089) ; Front Microbiol. 2020 Apr 07;11:592. (PMID: 32318050) ; Microbiol Mol Biol Rev. 2006 Dec;70(4):939-1031. (PMID: 17158705) ; Front Microbiol. 2019 Mar 20;10:513. (PMID: 30949142) ; Stem Cell Reports. 2018 Oct 9;11(4):852-860. (PMID: 30197117) ; J Biol Chem. 2006 Dec 1;281(48):36929-36. (PMID: 16990261) Grant Information: 31671946 National Natural Science Foundation of China Contributed Indexing: Keywords: Vibrio parahaemolyticus; foodborne pathogen; prophage; transcriptome Entry Date(s): Date Created: 20240210 Date Completed: 20240214 Latest Revision: 20240214 Update Code: 20240214 PubMed Central ID: PMC10855970

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Biological Function of Prophage-Related Gene Cluster Δ VpaChn25_RS25055 ~Δ VpaChn25_0714 of Vibrio parahaemolyticus CHN25.