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Conjugative transposons in Gram-negative bacteria have a significant role in the dissemination of antibiotic-resistance-conferring genes between bacteria. This study aims to genomically characterize plasmids and conjugative transposons carrying integrons in clinical isolates of Klebsiella pneumoniae. The genetic composition of conjugative transposons and phenotypic assessment of 50 multidrug-resistant K. pneumoniae isolates from a tertiary-care hospital (SQUH), Muscat, Oman, were investigated. Horizontal transferability was investigated by filter mating conjugation experiments. Whole-genome sequencing (WGS) was performed to determine the sequence type (ST), acquired resistome, and plasmidome of integron-carrying strains. Class 1 integrons were detected in 96% of isolates and, among integron-positive isolates, 18 stains contained variable regions. Horizontal transferability by conjugation confirmed the successful transfer of integrons between cells and WGS confirmed their presence in conjugative plasmids. Dihydrofolate reductase ( dfrA14 ) was the most prevalent (34.8%) gene cassette in class 1 integrons. MLST analysis detected predominantly ST-231 and ST-395. Bla OXA-232 and bla CTX-M-15 were the most frequently detected carbapenemases and beta-lactamases in the sequenced isolates. This study highlighted the high transmissibility of MDR-conferring conjugative plasmids in clinical isolates of K. pneumoniae . Therefore, the wise use of antibiotics and the adherence to effective infection control measures are necessary to limit the further dissemination of multidrug-resistant bacteria.

Titel:	Comparative Genomic Analysis Reveals the Emergence of ST-231 and ST-395 Klebsiella pneumoniae Strains Associated with the High Transmissibility of bla <subscript>KPC</subscript> Plasmids.
Autor/in / Beteiligte Person:	Al-Muzahmi, M ; Rizvi, M ; Al-Quraini, M ; Al-Muharrmi, Z ; Al-Jabri, Z
Link:	Zum Volltext
Zeitschrift:	Microorganisms, Jg. 11 (2023-09-27), Heft 10
Veröffentlichung:	Basel, Switzerland : MDPI AG, [2013]-, 2023
Medientyp:	academicJournal
ISSN:	2076-2607 (print)
DOI:	10.3390/microorganisms11102411
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Microorganisms] 2023 Sep 27; Vol. 11 (10). <i>Date of Electronic Publication: </i>2023 Sep 27. References: Avicenna J Med Biotechnol. 2020 Apr-Jun;12(2):124-131. (PMID: 32431797) ; J Glob Antimicrob Resist. 2017 Jun;9:96-99. (PMID: 28458051) ; FEMS Microbiol Lett. 1999 Feb 15;171(2):215-21. (PMID: 10077847) ; PLoS One. 2014 Aug 11;9(8):e104984. (PMID: 25110940) ; Southeast Asian J Trop Med Public Health. 2008 May;39(3):419-24. (PMID: 18564680) ; Clin Microbiol Infect. 2004 Apr;10(4):272-88. (PMID: 15059115) ; Clin Microbiol Infect. 2012 May;18(5):E144-8. (PMID: 22404169) ; BMC Infect Dis. 2019 Jan 7;19(1):20. (PMID: 30616536) ; Antibiotics (Basel). 2022 Jan 25;11(2):. (PMID: 35203756) ; Antimicrob Agents Chemother. 2015 Jan;59(1):536-43. (PMID: 25385117) ; Antimicrob Agents Chemother. 2010 Jan;54(1):24-38. (PMID: 19721065) ; J Antimicrob Chemother. 2023 May 3;78(5):1259-1264. 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Comparative Genomic Analysis Reveals the Emergence of ST-231 and ST-395 Klebsiella pneumoniae Strains Associated with the High Transmissibility of bla <subscript>KPC</subscript> Plasmids.