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Background: Filamentous fungi possess a rich CAZymes system, which is widely studied and applied in the bio-conversion of plant biomass to alcohol chemicals. Carbon source acquisition is the fundamental driver for CAZymes-producing sustainability and secondary metabolism, therefore, a deeper insight into the regulatory network of sugar transport in filamentous fungi has become urgent.

Results: This study reports an important linkage of sulfur assimilation to lignocellulose response of filamentous fungus. Inorganic sulfur addition facilitated biodegradation of rice straw by Trichoderma guizhouense NJAU4742. Cysteine and glutathione were revealed as major intracellular metabolites responsive to sulfur addition by metabolomics, cysteine content was increased in this process and glutathione increased correspondingly. Two membrane sugar transporter genes, Tgmst1 and Tgmst2, were identified as the critical response genes significantly up-regulated when intracellular cysteine increased. Tgmst1 and Tgmst2 were both positively regulated by the glucose regulation-related protein (GRP), up-regulation of both Tgmst1 and Tggrp can cause a significant increase in intracellular glucose. The transcriptional regulatory function of GRP mainly relied on GSH-induced glutathionylation, and the transcription activating efficiency was positively related to the glutathionylation level, furthermore, DTT-induced deglutathionylation resulted in the down-regulation of downstream genes.

Conclusions: Inorganic sulfur addition induces a rise in intracellular Cys content, and the conversion of cysteine to glutathione caused the increase of glutathionylation level of GRP, which in turn up-regulated Tgmst1 and Tgmst2. Subsequently, the sugar transport efficiency of single cells was improved, which facilitated the maintenance of vigorous CAZymes metabolism and the straw-to-biomass conversion.

Titel:	Cysteine facilitates the lignocellulolytic response of Trichoderma guizhouense NJAU4742 by indirectly up-regulating membrane sugar transporters.
Autor/in / Beteiligte Person:	Liu, Y ; Li, T ; Zhu, H ; Zhou, Y ; Shen, Q ; Liu, D
Link:	Zum Volltext
Zeitschrift:	Biotechnology for biofuels and bioproducts, Jg. 16 (2023-10-27), Heft 1, S. 159
Veröffentlichung:	[London] : BioMed Central, [2022]-, 2023
Medientyp:	academicJournal
ISSN:	2731-3654 (electronic)
DOI:	10.1186/s13068-023-02418-9
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biotechnol Biofuels Bioprod] 2023 Oct 27; Vol. 16 (1), pp. 159. <i>Date of Electronic Publication: </i>2023 Oct 27. References: Biotechnol Biofuels. 2018 Dec 03;11:323. (PMID: 30534201) ; Int J Mol Sci. 2021 May 06;22(9):. (PMID: 34066424) ; Free Radic Biol Med. 2009 Jun 15;46(12):1633-8. (PMID: 19328229) ; Carbohydr Polym. 2013 Jan 16;91(2):646-50. (PMID: 23121959) ; J Fungi (Basel). 2021 Nov 23;7(12):. (PMID: 34946980) ; Plant Cell Environ. 2022 Mar;45(3):969-984. (PMID: 34800291) ; Can J Physiol Pharmacol. 2012 Aug;90(8):1061-4. (PMID: 22783875) ; Nat Commun. 2021 Dec 7;12(1):7094. (PMID: 34876574) ; J Biol Chem. 2013 Nov 15;288(46):32861-72. (PMID: 24085297) ; Biochem J. 2010 Dec 1;432(2):399-406. (PMID: 20854260) ; Nat Commun. 2014 Aug 04;5:4521. (PMID: 25088546) ; Mol Plant. 2009 Mar;2(2):218-35. (PMID: 19825609) ; Bioresour Technol. 2020 Dec;317:123992. (PMID: 32799087) ; Curr Opin Chem Biol. 2016 Dec;35:51-57. (PMID: 27614174) ; FEMS Microbiol Rev. 2008 Nov;32(6):1010-32. (PMID: 18811658) ; J Biol Chem. 2016 Dec 30;291(53):27160-27169. (PMID: 27856640) ; Nat Med. 2004 Nov;10(11):1200-7. (PMID: 15489859) ; J Biol Chem. 1999 May 28;274(22):15857-64. (PMID: 10336489) ; PLoS Genet. 2018 Apr 9;14(4):e1007322. (PMID: 29630596) ; Nat Commun. 2019 Feb 1;10(1):541. (PMID: 30710085) ; Environ Microbiol. 2023 Feb;25(2):331-351. (PMID: 36367399) ; Front Immunol. 2018 Aug 24;9:1845. (PMID: 30197639) ; Int J Mol Sci. 2013 Oct 17;14(10):20845-76. (PMID: 24141185) ; Appl Microbiol Biotechnol. 2018 Oct;102(19):8203-8211. (PMID: 30046857) ; Bioresour Technol. 2012 May;111:261-7. (PMID: 22406100) ; J Nutr Biochem. 2007 Aug;18(8):519-24. (PMID: 17142027) ; Biotechnol Adv. 2020 Nov 1;43:107551. (PMID: 32437733) ; Biotechnol Adv. 2019 Jul - Aug;37(4):519-529. (PMID: 30576717) ; FEMS Microbiol Rev. 2005 Sep;29(4):719-39. (PMID: 16102600) ; Proc Natl Acad Sci U S A. 2012 May 8;109(19):7397-402. (PMID: 22532664) ; Free Radic Res. 2005 Jun;39(6):573-80. (PMID: 16036334) ; Biotechnol Biofuels. 2017 Jun 12;10:152. (PMID: 28616076) ; Annu Rev Biochem. 2013;82:323-55. (PMID: 23746257) ; J Mol Biol. 2015 Apr 10;427(7):1575-88. (PMID: 25683596) ; Biochemistry. 2001 Nov 27;40(47):14134-42. (PMID: 11714266) ; J Biol Chem. 2020 Jun 12;295(24):8302-8324. (PMID: 32332101) ; Adv Appl Microbiol. 2021;114:73-109. (PMID: 33934853) ; J Allergy Clin Immunol. 2014 Jan;133(1):198-206.e1-9. (PMID: 24290279) ; Bioinformatics. 2011 Nov 1;27(21):2987-93. (PMID: 21903627) ; Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6987-92. (PMID: 21482798) ; Biotechnol Biofuels. 2016 Sep 26;9:204. (PMID: 27708711) ; Bioresour Technol. 2016 Dec;222:413-421. (PMID: 27744242) ; Nat Rev Mol Cell Biol. 2016 Feb;17(2):123-32. (PMID: 26758938) ; J Immunol. 2012 Aug 1;189(3):1253-64. (PMID: 22753936) ; FEMS Yeast Res. 2002 May;2(2):183-201. (PMID: 12702307) ; Mol Plant Microbe Interact. 2021 Jun;34(6):631-644. (PMID: 33496609) ; Nat Microbiol. 2018 Apr;3(4):402-414. (PMID: 29588541) ; J Med Chem. 2010 Jun 24;53(12):4585-602. (PMID: 20334364) ; Nucleic Acids Res. 2008 Jan;36(Database issue):D480-4. (PMID: 18077471) ; Biotechnol Biofuels. 2018 Apr 02;11:84. (PMID: 29619080) ; J Biol Chem. 2009 Jul 3;284(27):17897-901. (PMID: 19342379) ; Biochem Biophys Res Commun. 2015 May 8;460(3):663-9. (PMID: 25817789) ; Biotechnol Biofuels. 2013 Oct 16;6(1):149. (PMID: 24131596) ; Biotechnol Biofuels. 2014 Feb 28;7(1):31. (PMID: 24581151) ; BMC Biotechnol. 2015 Jul 22;15:64. (PMID: 26198409) ; Fungal Genet Biol. 2014 Nov;72:2-9. (PMID: 25192611) ; Nat Protoc. 2011 Oct 27;6(11):1818-33. (PMID: 22036884) ; Trends Biochem Sci. 2009 Feb;34(2):85-96. (PMID: 19135374) ; Science. 2007 Feb 9;315(5813):804-7. (PMID: 17289988) ; Methods Mol Biol. 2018;1689:29-42. (PMID: 29027162) ; World J Microbiol Biotechnol. 2018 Mar 17;34(4):51. (PMID: 29550883) Grant Information: XUEKEN2023039 Nanjing Agricultural University; 32172680 National Natural Science Foundation of China Contributed Indexing: Keywords: Gene manipulation; Glutathionylation; Lignocellulolytic response; Multiomics; Protein-DNA interactions; Sugar transporter; T. guizhouense NJAU4742 Entry Date(s): Date Created: 20231027 Latest Revision: 20231103 Update Code: 20240514 PubMed Central ID: PMC10612256

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Cysteine facilitates the lignocellulolytic response of Trichoderma guizhouense NJAU4742 by indirectly up-regulating membrane sugar transporters.