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Background: many previous studies have demonstrated the therapeutic potential of N. sativa total oil fractions, neutral lipids (NLs), glycolipids (GLs), phospholipids (PLs), and unsaponifiable (IS) in asthma patients. We therefore tested its effect on airway smooth muscle (ASM) cells by observing its ability to regulate the production of glucocorticoid (GC)-insensitive chemokines in cells treated with TNF-α/IFN-γ, and its antioxidative and reactive oxygen species (ROS) scavenging properties.

Materials and Methods: the cytotoxicity of N. sativa oil fractions was assessed using an MTT assay. ASM cells were treated with TNF-α/IFN-γ for 24 h in the presence of different concentrations of N. sativa oil fractions. An ELISA assay was used to determine the effect of N. sativa oil fractions on chemokine production (CCL5, CXCL-10, and CXCL-8). The scavenging effect of N. sativa oil fractions was evaluated on three reactive oxygen species (ROS), O 2 • - , OH•, and H 2 O 2 .

Results: our results show that different N. sativa oil fractions used at 25 and 50 µg/mL did not affect cell viability. All fractions of N. sativa oil inhibited chemokines in a concentration-dependent manner. Interestingly, the total oil fraction showed the most significant effect of chemokine inhibition, and had the highest percentage of ROS scavenging effect.

Conclusion: these results suggest that N. sativa oil modulates the proinflammatory actions of human ASM cells by inhibiting the production of GC-insensitive chemokines.

Titel:	Effects of Nigella sativa Oil Fractions on Reactive Oxygen Species and Chemokine Expression in Airway Smooth Muscle Cells.
Autor/in / Beteiligte Person:	Mosbah, A ; Khither, H ; Mosbah, C ; Slimani, A ; Mahrouk, A ; Akkal, S ; Nieto, G
Link:	Zum Volltext
Zeitschrift:	Plants (Basel, Switzerland), Jg. 12 (2023-05-30), Heft 11
Veröffentlichung:	Basel, Switzerland : MDPI AG, [2012]-, 2023
Medientyp:	academicJournal
ISSN:	2223-7747 (print)
DOI:	10.3390/plants12112171
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Plants (Basel)] 2023 May 30; Vol. 12 (11). <i>Date of Electronic Publication: </i>2023 May 30. References: Phytomedicine. 2014 Apr 15;21(5):740-4. (PMID: 24268807) ; Nat Rev Mol Cell Biol. 2020 Jul;21(7):363-383. (PMID: 32231263) ; Phytother Res. 2003 Dec;17(10):1209-14. (PMID: 14669258) ; Nat Prod Res. 2019 Aug;33(15):2266-2270. (PMID: 30037274) ; Indian J Exp Biol. 2008 Dec;46(12):805-10. (PMID: 19245176) ; J Agric Food Chem. 2003 Nov 19;51(24):6961-9. (PMID: 14611155) ; Allergol Int. 2006 Sep;55(3):215-23. (PMID: 17075261) ; Free Radic Biol Med. 2022 May 20;185:97-119. (PMID: 35472411) ; Antioxid Redox Signal. 2008 Apr;10(4):785-97. (PMID: 18177234) ; Biomolecules. 2021 Dec 23;12(1):. (PMID: 35053168) ; Biomed Res Int. 2021 Jun 26;2021:9979419. (PMID: 34258287) ; Antioxidants (Basel). 2022 Nov 13;11(11):. (PMID: 36421423) ; Animal. 2012 Dec;6(12):2048-56. (PMID: 23031660) ; Carcinogenesis. 1989 Jun;10(6):1003-8. (PMID: 2470525) ; Phytother Res. 2017 Mar;31(3):403-409. (PMID: 28093815) ; Phytomedicine. 2010 Aug;17(10):707-13. (PMID: 20149611) ; Antioxidants (Basel). 2020 Oct 29;9(11):. (PMID: 33138148) ; Oxid Med Cell Longev. 2016;2016:4350965. (PMID: 26998193) ; Proc Am Thorac Soc. 2008 Jan 1;5(1):106-12. (PMID: 18094092) ; Proc Am Thorac Soc. 2005;2(4):347-54; discussion 371-2. (PMID: 16267361) ; Biochem Pharmacol. 1988 Mar 1;37(5):837-41. (PMID: 2830882) ; Poult Sci. 2020 Mar;99(3):1491-1501. (PMID: 32111317) ; J Immunol. 2013 Sep 1;191(5):2624-2636. (PMID: 23904164) ; Mol Aspects Med. 2022 Jun;85:100969. (PMID: 34090658) ; Eur J Immunol. 2012 Nov;42(11):2827-33. (PMID: 23041833) ; Phytother Res. 2021 Jun;35(6):2968-2996. (PMID: 33455047) ; Nutrients. 2018 Jul 26;10(8):. (PMID: 30049997) ; J Immunol. 1997 Feb 15;158(4):1841-7. (PMID: 9029124) ; J Clin Lipidol. 2016 Sep-Oct;10(5):1203-11. (PMID: 27678438) ; Antioxidants (Basel). 2020 Jul 20;9(7):. (PMID: 32698505) ; Int Arch Allergy Immunol. 1994;104 Suppl 1(1):52-3. (PMID: 7512397) ; Curr Opin Allergy Clin Immunol. 2008 Feb;8(1):49-56. (PMID: 18188018) ; Blood. 1996 Oct 15;88(8):3195-9. (PMID: 8874220) ; Nutrition. 2016 Jun;32(6):649-55. (PMID: 26827238) ; Asian Pac J Trop Biomed. 2013 May;3(5):337-52. (PMID: 23646296) ; Saudi Med J. 2016 Mar;37(3):235-44. (PMID: 26905343) ; Mol Aspects Med. 2022 Jun;85:101026. (PMID: 34625291) ; Pharmacol Ther. 2020 Sep;213:107589. (PMID: 32473159) ; Respir Physiol Neurobiol. 2003 Sep 16;137(2-3):209-22. (PMID: 14516727) ; Curr Drug Targets Inflamm Allergy. 2002 Sep;1(3):291-315. (PMID: 14561194) ; BMC Pharmacol. 2004 Feb 25;4:3. (PMID: 15070429) ; Molecules. 2020 Sep 26;25(19):. (PMID: 32993187) ; Int Immunopharmacol. 2005 Dec;5(13-14):1749-70. (PMID: 16275613) ; Phytother Res. 2004 Mar;18(3):195-9. (PMID: 15103664) ; Clin Exp Allergy. 2015 Nov;45(11):1637-46. (PMID: 26017278) ; Mol Pharmacol. 2004 Aug;66(2):322-9. (PMID: 15266023) ; Allergy. 2017 Jan;72(1):126-136. (PMID: 27501780) ; Ann Saudi Med. 2017 Jan-Feb;37(1):64-71. (PMID: 28151459) Contributed Indexing: Keywords: Nigella sativa oil; airway smooth muscle cells; asthma; chemokines; inflammation; reactive oxygen species Entry Date(s): Date Created: 20230610 Latest Revision: 20230612 Update Code: 20231215 PubMed Central ID: PMC10255787

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Effects of Nigella sativa Oil Fractions on Reactive Oxygen Species and Chemokine Expression in Airway Smooth Muscle Cells.