Einzeltreffer — DigiBib

Studies already revealed that some E3 ubiquitin ligases participated in the immune response after viral infection by regulating the type I interferon (IFN) pathway. Here, we demonstrated that type I interferon signaling enhanced the translocation of ETS1 to the nucleus and the promoter activity of E3 ubiquitin ligase DTX3L (deltex E3 ubiquitin ligase 3L) after virus infection and thus increased the expression of DTX3L. Further experiments suggested that DTX3L ubiquitinated TBK1 at K30 and K401 sites on K63-linked ubiquitination pathway. DTX3L was also necessary for mediating the phosphorylation of TBK1 through binding with the tyrosine kinase SRC: both together enhanced the activation of TBK1. Therefore, DTX3L, being an important positive-feedback regulator of type I interferon, exerted a key role in antiviral response. IMPORTANCE Our present study evaluated DTX3L as an antiviral molecule by promoting IFN production and establishing an IFN-β-ETS1-DTX3L-TBK1 positive-feedback loop as a novel immunomodulatory step to enhance interferon signaling and inhibit respiratory syncytial virus (RSV) infection. Our finding enriches and complements the biological function of DTX3L and provides a new strategy to protect against lung diseases such as bronchiolitis and pneumonia that develop with RSV.

Competing Interests: The authors declare no conflict of interest.

Titel:	DTX3L Enhances Type I Interferon Antiviral Response by Promoting the Ubiquitination and Phosphorylation of TBK1.
Autor/in / Beteiligte Person:	Huang, J ; Chen, Z ; Ye, Y ; Shao, Y ; Zhu, P ; Li, X ; Ma, Y ; Xu, F ; Zhou, J ; Wu, M ; Gao, X ; Yang, Y ; Zhang, J ; Hao, C
Link:	Zum Volltext
Zeitschrift:	Journal of virology, Jg. 97 (2023-06-29), Heft 6, S. e0068723
Veröffentlichung:	Washington Dc : American Society For Microbiology ; <i>Original Publication</i>: Baltimore, American Society for Microbiology., 2023
Medientyp:	academicJournal
ISSN:	1098-5514 (electronic)
DOI:	10.1128/jvi.00687-23
Schlagwort:	Phosphorylation Ubiquitination Respiratory Syncytial Viruses Immunity, Innate Interferon Type I metabolism Protein Serine-Threonine Kinases metabolism Ubiquitin-Protein Ligases metabolism Respiratory Syncytial Virus Infections immunology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Virol] 2023 Jun 29; Vol. 97 (6), pp. e0068723. <i>Date of Electronic Publication: </i>2023 May 31. MeSH Terms: Immunity, Innate* ; Interferon Type I* / metabolism ; Protein Serine-Threonine Kinases* / metabolism ; Ubiquitin-Protein Ligases* / metabolism ; Respiratory Syncytial Virus Infections* / immunology ; Phosphorylation ; Ubiquitination ; Respiratory Syncytial Viruses References: Nat Rev Immunol. 2008 Jul;8(7):559-68. (PMID: 18575461) ; PLoS One. 2013 Nov 26;8(11):e81695. (PMID: 24303065) ; Nat Immunol. 2016 Dec;17(12):1342-1351. (PMID: 27776110) ; J Immunol Methods. 1997 Mar 10;202(1):49-57. (PMID: 9075771) ; Structure. 2013 Jul 2;21(7):1137-48. (PMID: 23746807) ; Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):14046-51. (PMID: 18780793) ; Front Immunol. 2018 Oct 25;9:2479. (PMID: 30410495) ; Trends Immunol. 2019 Dec;40(12):1134-1148. (PMID: 31735513) ; Cell Rep. 2013 Mar 28;3(3):747-58. (PMID: 23453972) ; Mol Cancer. 2015 Dec 11;14:207. (PMID: 26654227) ; Bull World Health Organ. 2008 May;86(5):408-16. (PMID: 18545744) ; Front Immunol. 2019 Mar 26;10:566. (PMID: 30972063) ; Nat Immunol. 2009 Jul;10(7):744-52. (PMID: 19483718) ; J Biol Chem. 2003 Jun 13;278(24):21930-7. (PMID: 12670957) ; Immunity. 2010 Dec 14;33(6):878-89. (PMID: 21145761) ; J Virol. 2018 Jan 30;92(4):. (PMID: 29187546) ; Lancet. 2005 Mar 26-Apr 1;365(9465):1147-52. (PMID: 15794969) ; Tumour Biol. 2017 Jun;39(6):1010428317703941. (PMID: 28653881) ; J Exp Med. 2015 May 4;212(5):699-714. (PMID: 25897172) ; J Gen Virol. 2008 Jan;89(Pt 1):1-47. (PMID: 18089727) ; Mol Cell Biol. 2013 Feb;33(4):845-57. (PMID: 23230272) ; Ann N Y Acad Sci. 2007 Oct;1114:36-47. (PMID: 17986575) ; Nat Commun. 2017 Mar 13;8:14654. (PMID: 28287082) ; Mol Cell. 2017 May 18;66(4):503-516.e5. (PMID: 28525742) ; J Immunol. 2012 Dec 15;189(12):5942-53. (PMID: 23136205) ; J Virol. 2007 Sep;81(18):9790-800. (PMID: 17626092) ; Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552. (PMID: 34723319) ; Nat Immunol. 2015 Dec;16(12):1215-27. (PMID: 26479788) ; Mol Cell. 2009 Oct 9;36(1):110-20. (PMID: 19818714) ; Cell Rep. 2013 Mar 28;3(3):734-46. (PMID: 23453971) ; Mol Cancer. 2014 May 27;13:125. (PMID: 24886089) ; Sci Signal. 2017 Jan 03;10(460):. (PMID: 28049762) ; Eur J Pediatr. 2019 Feb;178(2):131-138. (PMID: 30610420) Contributed Indexing: Keywords: DTX3L; antiviral response; deltex E3 ubiquitin ligase 3L; respiratory syncytial virus; type I interferon Substance Nomenclature: 0 (Interferon Type I) ; EC 2.7.11.1 (Protein Serine-Threonine Kinases) ; EC 2.3.2.27 (Ubiquitin-Protein Ligases) Entry Date(s): Date Created: 20230531 Date Completed: 20230712 Latest Revision: 20231201 Update Code: 20231215 PubMed Central ID: PMC10308958

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

DTX3L Enhances Type I Interferon Antiviral Response by Promoting the Ubiquitination and Phosphorylation of TBK1.