Einzeltreffer — DigiBib

Background: Despite the global prevalence of Klebsiella pneumoniae Carbapenemase (KPC)-type class A β-lactamases, occurrences of KPC-3-producing isolates in China remain infrequent. This study aims to explore the emergence, antibiotic resistance profiles, and plasmid characteristics of bla KPC-3 -carrying Pseudomonas aeruginosa.

Methods: Species identification was performed by MALDI-TOF-MS, and antimicrobial resistance genes (ARGs) were identified by polymerase chain reaction (PCR). The characteristics of the target strain were detected by whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST). Plasmids were analyzed by S1-nuclease pulsed-field gel electrophoresis(S1-PFGE), Southern blotting and transconjugation experiment.

Results: Five P. aeruginosa strains carrying bla KPC-3 were isolated from two Chinese patients without a history of travelling to endemic areas. All strains belonged to the novel sequence type ST1076. The bla KPC-3 was carried on a 395-kb IncP-2 megaplasmid with a conserved structure (IS6100-ISKpn27-bla KPC-3 -ISKpn6-korC-klcA), and this genetic sequence was identical to many plasmid-encoded KPC of Pseudomonas species. By further analyzing the genetic context, it was supposed that the original of bla KPC-3 in our work was a series of mutation of bla KPC-2 .

Conclusions: The emergence of a multidrug resistance IncP-2 megaplasmid and clonal transmission of bla KPC-3 -producing P. aeruginosa in China underlined the crucial need for continuous monitoring of bla KPC-3 for prevention and control of its further dissemination in China.

Titel:	Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid.
Autor/in / Beteiligte Person:	Ge, H ; Qiao, J ; Zheng, J ; Xu, H ; Liu, R ; Zhao, J ; Chen, R ; Li, C ; Guo, X ; Zheng, B
Link:	Zum Volltext
Zeitschrift:	Annals of clinical microbiology and antimicrobials, Jg. 22 (2023-04-29), Heft 1, S. 31
Veröffentlichung:	London : BioMed Central, [2002]-, 2023
Medientyp:	academicJournal
ISSN:	1476-0711 (electronic)
DOI:	10.1186/s12941-023-00577-z
Schlagwort:	Humans Pseudomonas aeruginosa genetics Multilocus Sequence Typing beta-Lactamases genetics Bacterial Proteins genetics Plasmids genetics China epidemiology Anti-Bacterial Agents pharmacology Klebsiella pneumoniae Klebsiella Infections epidemiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Ann Clin Microbiol Antimicrob] 2023 Apr 29; Vol. 22 (1), pp. 31. <i>Date of Electronic Publication: </i>2023 Apr 29. MeSH Terms: Klebsiella pneumoniae* ; Klebsiella Infections* / epidemiology ; Humans ; Pseudomonas aeruginosa / genetics ; Multilocus Sequence Typing ; beta-Lactamases / genetics ; Bacterial Proteins / genetics ; Plasmids / genetics ; China / epidemiology ; Anti-Bacterial Agents / pharmacology References: J Microbiol Methods. 2005 Dec;63(3):219-28. (PMID: 15935499) ; Trends Microbiol. 2011 Aug;19(8):419-26. (PMID: 21664819) ; Antimicrob Agents Chemother. 2015 Nov;59(11):7086-9. (PMID: 26282422) ; Environ Pollut. 2020 May;260:114041. (PMID: 32006889) ; Front Microbiol. 2022 Oct 20;13:980191. (PMID: 36338063) ; Antimicrob Agents Chemother. 2004 Dec;48(12):4793-9. (PMID: 15561858) ; Antimicrob Agents Chemother. 2001 Apr;45(4):1151-61. (PMID: 11257029) ; New Microbes New Infect. 2019 Feb 23;29:100516. (PMID: 30949345) ; Front Microbiol. 2016 Mar 10;7:310. (PMID: 27014233) ; Antimicrob Agents Chemother. 2016 Mar 25;60(4):2519-23. (PMID: 26787700) ; Emerg Infect Dis. 2022 Jul;28(7):1421-1430. (PMID: 35731165) ; Microb Ecol. 2020 Jan;79(1):30-37. (PMID: 31055618) ; Microbiol Spectr. 2023 Feb 14;11(1):e0384622. (PMID: 36472421) ; Infect Drug Resist. 2021 Aug 10;14:3011-3017. (PMID: 34413655) ; Pathogens. 2021 Apr 17;10(4):. (PMID: 33920533) ; J Antimicrob Chemother. 2021 Jan 1;76(1):220-225. (PMID: 33038895) ; Antibiotics (Basel). 2020 Nov 13;9(11):. (PMID: 33202755) ; J Antimicrob Chemother. 2021 May 12;76(6):1455-1458. (PMID: 33758948) ; Emerg Microbes Infect. 2021 Dec;10(1):442-449. (PMID: 33620296) ; Antimicrob Agents Chemother. 2021 Mar 18;65(4):. (PMID: 33526490) ; EBioMedicine. 2017 May;19:98-106. (PMID: 28479289) ; Pathogens. 2021 Oct 12;10(10):. (PMID: 34684258) ; Antimicrob Agents Chemother. 2009 Sep;53(9):4048-50. (PMID: 19581459) ; Antibiotics (Basel). 2019 Aug 19;8(3):. (PMID: 31430964) ; Microbiol Spectr. 2022 Feb 23;10(1):e0199221. (PMID: 35171033) ; J Hosp Infect. 2016 Sep;94(1):2-7. (PMID: 27451039) ; J Antimicrob Chemother. 2021 Jan 19;76(2):380-384. (PMID: 33202023) ; Microbiology (Reading). 1999 Sep;145 ( Pt 9):2203-2208. (PMID: 10517573) ; Nat Commun. 2020 Mar 13;11(1):1370. (PMID: 32170080) ; J Hosp Infect. 2018 Aug;99(4):413-418. (PMID: 29621600) ; Semin Diagn Pathol. 2019 May;36(3):182-186. (PMID: 31056277) ; J Glob Antimicrob Resist. 2018 Dec;15:109-110. (PMID: 30213719) ; Antibiotics (Basel). 2021 Sep 25;10(10):. (PMID: 34680744) ; Antimicrob Agents Chemother. 2019 May 24;63(6):. (PMID: 30936106) ; Front Microbiol. 2021 Mar 15;12:585716. (PMID: 33790873) ; Antimicrob Agents Chemother. 2017 Feb 23;61(3):. (PMID: 28031201) ; Antimicrob Agents Chemother. 2020 Feb 21;64(3):. (PMID: 31844014) ; Infect Drug Resist. 2019 May 14;12:1285-1288. (PMID: 31190916) ; J Glob Antimicrob Resist. 2018 Dec;15:166-168. (PMID: 30291901) ; Pathogens. 2021 Mar 12;10(3):. (PMID: 33809215) ; Front Cell Infect Microbiol. 2022 May 26;12:774494. (PMID: 35719362) ; Antimicrob Agents Chemother. 2017 Jul 25;61(8):. (PMID: 28559268) ; Antimicrob Agents Chemother. 2008 Apr;52(4):1257-63. (PMID: 18227185) ; Infect Dis Ther. 2022 Aug;11(4):1725-1740. (PMID: 35689153) ; Rev Argent Microbiol. 2020 Jul - Sep;52(3):211-216. (PMID: 31874719) ; J Antimicrob Chemother. 2019 Feb 1;74(2):321-325. (PMID: 30418551) ; Antimicrob Agents Chemother. 2021 Nov 17;65(12):e0116021. (PMID: 34606331) Grant Information: LHGJ20190232 Henan Province Medical Science and Technology Research Project Joint Construction Project; LHGJ20190232 Henan Province Medical Science and Technology Research Project Joint Construction Project; LHGJ20190232 Henan Province Medical Science and Technology Research Project Joint Construction Project; LHGJ20190232 Henan Province Medical Science and Technology Research Project Joint Construction Project; LHGJ20190232 Henan Province Medical Science and Technology Research Project Joint Construction Project; 82072314 National Natural Science Foundation of China; 82072314 National Natural Science Foundation of China; JNL-2022011B the Research Project of Jinan Microecological Biomedicine Shandong Laboratory; JNL-2022011B the Research Project of Jinan Microecological Biomedicine Shandong Laboratory; 2022ZFJH003 the Fundamental Research Funds for the Central Universities; 2022ZFJH003 the Fundamental Research Funds for the Central Universities; 2019-I2M-5-045 CAMS Innovation Fund for Medical Sciences; 2019-I2M-5-045 CAMS Innovation Fund for Medical Sciences Contributed Indexing: Keywords: Bla KPC-3; Carbapenemase; Clonal dissemination; IncP-2 megaplasmid; Pseudomonas aeruginosa; ST1076 Substance Nomenclature: EC 3.5.2.6 (beta-Lactamases) ; 0 (Bacterial Proteins) ; 0 (Anti-Bacterial Agents) Entry Date(s): Date Created: 20230429 Date Completed: 20230501 Latest Revision: 20230502 Update Code: 20240513 PubMed Central ID: PMC10149002

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid.