MiR-217 targets TBK1 to modulate inflammatory response in grass carp following infection with Aeromonas hydrophila.
In: Developmental and comparative immunology, Jg. 143 (2023-06-01), S. 104583
Online
academicJournal
Zugriff:
The current study demonstrated that miR-217 modulates inflammation in grass carp (Ctenopharyngodon Idella) infected with Aeromonas hydrophila. Bacterial infection in grass carp causes high levels septicemia, which arises with systemic inflammatory responses. As a result leading to the development of hyperinflammatory state which causes septic shocks and lethality. Based on the current data, TBK1 was confirmed to be the target gene of miR-217 after a successful gene expression profiling or luciferase experiment and miR-217 expression in CIK cells. Furthermore, TargetscanFish6.2 predicted TBK1 as the target gene of miR-217. Quantitative real-time PCR was performed to measure miR-217 expression levels for six immune-related genes and miR-217 regulation in grass carp after A. hydrophila infection in CIK cells. In grass carp CIK cells, the expression of TBK1 mRNA was up-regulated under poly (I: C) stimulation. The transcriptional analysis of the immune-related genes demonstrated that the expression levels of tumor necrosis factor-α (TNF-α), interferon (ifn), interleukin 6 (il-6), interleukin 8 (il-8), and interleukin 12 (il-12) were altered after a successful transfection into the CIK cells, proposing that miRNA regulates immune responses in grass carp. These results provided a theoretical basis and contribute to further studies on the pathogenesis and host defensive system during A. hydrophila infection.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2022. Published by Elsevier Ltd.)
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MiR-217 targets TBK1 to modulate inflammatory response in grass carp following infection with Aeromonas hydrophila.
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Autor/in / Beteiligte Person: | Salifu, AR ; Pang, Y ; He, Y ; Liu, Y ; Shen, Y ; Xu, X ; Li, J |
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Zeitschrift: | Developmental and comparative immunology, Jg. 143 (2023-06-01), S. 104583 |
Veröffentlichung: | Tarrytown Ny : Elsevier Science ; <i>Original Publication</i>: Elmsford, N. Y., Pergamon Press., 1977-2004., 2023 |
Medientyp: | academicJournal |
ISSN: | 1879-0089 (electronic) |
DOI: | 10.1016/j.dci.2022.104583 |
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