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In this study, we evaluate a method for the KPC enzyme detection, using MALDI-TOF MS, for Enterobacterales. A total of 300 clinical Enterobacterales isolates were selected. The collection included 259 carbapenemase-producing (157 KPC and 102 non-KPC) and 41 carbapenemase non-producing isolates. Bacterial proteins were extracted from Mueller-Hinton agar plates using formic acid, isopropyl alcohol, and water (17:33:50). Samples were prepared with a double layer of synapinic acid. Analyses were performed using a Microflex LT mass spectrometer (Bruker Daltonics) and flexAnalysis 4.0 software (Bruker Daltonics). Statistical analyses were performed using SPSS Software. A distinctive peak at m/z 28,643-28,731 was found in all 157 KPC-producing isolates, and it was consistently absent in the 143 KPC non-producing group. KPC-producing peak intensities ranged from 77 to 3893. Considering an intensity cutoff value ≥ 120 for the presence of KPC, this methodology presented 98.09% and 97.90% of sensitivity and specificity, respectively.

Titel:	MALDI-TOF mass spectrometry for direct KPC detection among Enterobacterales.
Autor/in / Beteiligte Person:	Moreira, NK ; Wilhelm, CM ; Wink, PL ; Barth, AL ; Caierão, J
Link:	Zum Volltext
Zeitschrift:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], Jg. 53 (2022-12-01), Heft 4, S. 1907
Veröffentlichung:	2019- : Switzerland, AG : Springer International Publishing ; <i>Original Publication</i>: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia, 2022
Medientyp:	academicJournal
ISSN:	1678-4405 (electronic)
DOI:	10.1007/s42770-022-00798-y
Schlagwort:	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods Bacterial Proteins metabolism beta-Lactamases metabolism Enterobacteriaceae
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Braz J Microbiol] 2022 Dec; Vol. 53 (4), pp. 1907-1913. <i>Date of Electronic Publication: </i>2022 Jul 18. MeSH Terms: beta-Lactamases* / metabolism ; Enterobacteriaceae* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods ; Bacterial Proteins / metabolism References: Villegas MV, Jiménez A, Esparza G, Appel TM (2019) Carbapenemase-producing Enterobacteriaceae: a diagnostic, epidemiological and therapeutic challenge. Infectio 23:388–397. https://doi.org/10.22354/in.v23i4.808. (PMID: 10.22354/in.v23i4.808) ; Perez F, Bonomo RA (2019) Carbapenem-resistant Enterobacteriaceae: global action required. Lancet Infect Dis 19:561–562. https://doi.org/10.1016/S1473-3099(19)30210-5. (PMID: 10.1016/S1473-3099(19)30210-531047851) ; Logan LK, Weinstein RA (2017) The epidemiology of carbapenem-resistant Enterobacteriaceae: the impact and evolution of a global menace. 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(PMID: 10.1007/978-1-4939-1776-1_9) ; Papagiannitsis CC, Študentová V, Izdebski R, Oikonomou O, Pfeifer Y, Petinaki E, Hrabák J (2015) Matrix-assisted laser desorption ionization–time of flight mass spectrometry meropenem hydrolysis assay with NH 4 HCO 3 , a reliable tool for direct detection of carbapenemase activity. J Clin Microbiol 53:1731–1735. https://doi.org/10.1128/JCM.03094-14. (PMID: 10.1128/JCM.03094-14256945224400744) Grant Information: Prêmio Pesquisador Gaúcho Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul; FIPE 31638920800005327 Hospital de Clínicas de Porto Alegre Contributed Indexing: Keywords: Carbapenem-resistance; Enterobacterales; KPC; MALDI-TOF MS Substance Nomenclature: EC 3.5.2.6 (beta-Lactamases) ; 0 (Bacterial Proteins) Entry Date(s): Date Created: 20220719 Date Completed: 20221123 Latest Revision: 20230719 Update Code: 20240513 PubMed Central ID: PMC9679046

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MALDI-TOF mass spectrometry for direct KPC detection among Enterobacterales.