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Background: Ultraviolet B (UVB) causes photoaging of the skin, the appearance of wrinkles, spots, and alteration of the skin barrier. The main cells in the most superficial layer of the skin are the keratinocytes; these cells play an important role in protecting this organ.

Objective: The present study aimed to investigate the antioxidant activity of the hydrolysates from kafirin to inhibit UVB-induced responses in human keratinocytes cells (HaCaT).

Methods: Kafirin hydrolysates were produced by enzymatic hydrolysis with alcalase. The activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), in the HaCaT cell line in the presence of UVB and the effects of the hydrolysates against the UVB-induced response were evaluated. Furthermore, the peptides that were generated by hydrolysis were identified in silico using the BIOPEP database.

Results: Two protein sequences were identified (α-kafirin and the precursor protein of α-kafirin), in the kafirin extract. A degree of hydrolysis of 18.8% was obtained by hydrolyzing the kafirin extract with alcalase. The kafirin hydrolysates avoided the decrease in endogenous antioxidant enzymes such as SOD, CAT, and GPx reducing the oxidative stress generated by UVB. Using the BIOPEP-UWM database, we found 102 peptide sequences, and it has shown that the peptides have a large amount of hydrophobic amino acids such as proline, alanine, and glutamine, and amino acids with high antioxidant capacity.

Conclusion: These results suggest that the kafirin hydrolysates can be used as antioxidant agents to ameliorate UVB-induced skin keratinocytes cells' response in vitro, providing an alternative against UVB-induced photoaging.

Titel:	Antioxidant activity of kafirin hydrolysates on UVB irradiated human keratinocyte cells and in silico identification.
Autor/in / Beteiligte Person:	Castro-Jácome, TP ; Montalvo-González, E ; Chacón-López, MA ; Kalixto-Sánchez, MA ; Rivera, MDP ; López-García, UM ; Alcántara-Quintana, LE ; Tovar-Pérez, EG
Link:	Zum Volltext
Zeitschrift:	Photodermatology, photoimmunology & photomedicine, Jg. 39 (2023), Heft 1, S. 51-59
Veröffentlichung:	<2010-> : Oxford : Blackwell ; <i>Original Publication</i>: Copenhagen : Munksgaard, c1990-, 2023
Medientyp:	academicJournal
ISSN:	1600-0781 (electronic)
DOI:	10.1111/phpp.12815
Schlagwort:	Humans Peptides pharmacology Peptides chemistry Peptides metabolism Superoxide Dismutase metabolism Amino Acids metabolism Amino Acids pharmacology Ultraviolet Rays adverse effects Antioxidants pharmacology Antioxidants chemistry Keratinocytes metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Photodermatol Photoimmunol Photomed] 2023 Jan; Vol. 39 (1), pp. 51-59. <i>Date of Electronic Publication: </i>2022 Jul 12. MeSH Terms: Antioxidants* / pharmacology ; Antioxidants* / chemistry ; Keratinocytes* / metabolism ; Humans ; Peptides / pharmacology ; Peptides / chemistry ; Peptides / metabolism ; Superoxide Dismutase / metabolism ; Amino Acids / metabolism ; Amino Acids / pharmacology ; Ultraviolet Rays / adverse effects References: Lin S, Li L, Li M, Ma Y, Gu H, Chen X. Raffinose increases autophagy and reduces cell death in UVB-irradiated keratinocytes. J Photochem Photobiol B Biol. 2019;21:111653. ; Jaisin Y, Ratanachamnong P, Wongsawattkul O, Watthammawut A, Malaniyom K, Natewong S. Antioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cells. Life Sci. 2020;263:118607. ; Thitilertdecha N, Chaiwut P, Saewan N. 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Relationship between primary structure or spatial conformation and functional activity of antioxidant peptides from Pinctada fucata. Food Chem. 2018;264:108-117. Grant Information: Consejo Nacional de Ciencia y Tecnología Contributed Indexing: Keywords: UVB; antioxidant; kafirins; keratinocytes; protein hydrolysate Substance Nomenclature: 0 (Antioxidants) ; 0 (Peptides) ; EC 1.15.1.1 (Superoxide Dismutase) ; 0 (Amino Acids) Entry Date(s): Date Created: 20220708 Date Completed: 20230117 Latest Revision: 20230117 Update Code: 20240513

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Antioxidant activity of kafirin hydrolysates on UVB irradiated human keratinocyte cells and in silico identification.