[Chondrogenic and ameliorated inflammatory effects of chitosan-based biomimetic scaffold loaded with icariin].
In: Sheng wu gong cheng xue bao = Chinese journal of biotechnology, Jg. 38 (2022-06-25), Heft 6, S. 2308
Online
academicJournal
Zugriff:
Icariin (ICA) is a small molecule drug capable of promoting cartilage repair and ameliorating inflammation. Loading ICA into a biomaterial scaffold for cartilage tissue engineering will thus potentially enhance the biological functionality of the engineered scaffold. In this study, short fibers processed from electrospun poly(l-lactide- co -caprolactone) (PLCL) fibers which were prior coated with polydopamine (PDA), were mixed with citric acid doped chitosan solution (CC) for preparing short fibers reinforced chitosan hydrogel (PDA@PLCL/CC) by a freeze-thawing combined freeze-drying method. Thereafter, ICA was loaded into the PDA@PLCL/CC scaffold through physical adsorption to generate a newly engineered biomimetic cartilage scaffold (ICA-PDA@PLCL/CC). Finally, ICA-mediated chondrogenic and ameliorated inflammatory effects of the ICA-PDA@PLCL/CC scaffold were examined in vitro using rabbit chondrocytes. The results showed that the ICA-free PDA@PLCL/CC scaffold possessed appropriate pore size and porosity (> 80%), high water absorbance capacity and improved mechanical performance, and also promoted chondrocyte proliferation and adhesion. The ICA-laden ICA-PDA@PLCL/CC scaffold was evidenced to maintain cytomorphology, upregulate the expression of chondrogenic gene ( sox-9 ), glycosaminoglycan gene ( gag ), and type Ⅱ collagen gene ( col Ⅱ ) as well as the synthesis of the cartilage matrix. In the presence of a simulated inflammation, the ICA-PDA@PLCL/CC scaffold was found to reduce chondrocyte fibrosis, effectively downregulate the expression of proinflammatory factors interleukin-6 ( il-6 ), interleukin-1 ( il-1 ), and inducible nitric oxide synthase ( inos ) in chondrocytes. It can also reduce matrix metalloproteinase-3 ( mmp-3 ) expression and promote the synthesis of the extracellular matrix glycosaminoglycan (GAG) and type II collagen (Col II). The newly developed ICA-PDA@PLCL/CC scaffold may find applications in the regeneration and repair of cartilage defects.
Titel: |
[Chondrogenic and ameliorated inflammatory effects of chitosan-based biomimetic scaffold loaded with icariin].
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Autor/in / Beteiligte Person: | Li, H ; Wang, X ; Shen, Y ; Tang, H ; Tang, X ; Zhang, Y |
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Zeitschrift: | Sheng wu gong cheng xue bao = Chinese journal of biotechnology, Jg. 38 (2022-06-25), Heft 6, S. 2308 |
Veröffentlichung: | Beijing : Ke xue chu ban she,, 2022 |
Medientyp: | academicJournal |
ISSN: | 1872-2075 (electronic) |
DOI: | 10.13345/j.cjb.210838 |
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