Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain.
In: Journal of microbiology and biotechnology, Jg. 32 (2022-04-28), Heft 4, S. 437
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Zugriff:
In this study, to obtain icaritin with high pharmacological activities from icariin, which has a content ratio of over 58% in the total flavonoids of Epimedium herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in a medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30°C for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the optimal pH and temperature were 5.0 and 40°C. The enzyme Km and Vmax values for icariin were 15.63 mM and 55.56 mM/h. Moreover, the enzyme hydrolyzed the 7- O -glucosides of icariin into icariside II, and finally hydrolyzed 3- O -rhamnoside of icariside II into icaritin. The enzyme also hydrolyzed 7- O -glucosides of epimedin B to sagittatoside B, and then further hydrolyzed terminal 3- O -xyloside of sagittatoside B to icarisiede II, before finally hydrolyzing 3- O -rhamnoside of icarisiede II into icaritin. The enzyme only hydrolyzed 7- O -glucoside of epimedin A or epimedin C into sagittatoside A or sagittatoside C. It is possible to prepare icaritin from the high-content icariin in Epimedium herb using this enzyme. When 2.5% icariin was reacted at 40°C for 18-20 h by the low-cost crude enzyme, 5.04 g icaritin with 98% purity was obtained from 10 g icariin. Also, the icaritin molar yield was 92.5%. Our results showed icaritin was successfully produced via cost-effective and relatively simple methods from icariin by crude enzyme. Our results should be very useful for the development of medicines from Epimedium herb.
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Icaritin Preparation from Icariin by a Special Epimedium Flavonoid-Glycosidase from Aspergillus sp.y848 Strain.
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Autor/in / Beteiligte Person: | Wang, Z ; Liu, C ; Yu, H ; Wu, B ; Huai, B ; Zhuang, Z ; Sun, C ; Xu, L ; Jin, F |
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Zeitschrift: | Journal of microbiology and biotechnology, Jg. 32 (2022-04-28), Heft 4, S. 437 |
Veröffentlichung: | 2002- : Seoul : Korean Society for Microbiology and Biotechnology ; <i>Original Publication</i>: Seoul : Korean Society for Applied Microbiology, 1991-2001., 2022 |
Medientyp: | academicJournal |
ISSN: | 1738-8872 (electronic) |
DOI: | 10.4014/jmb.2112.12036 |
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