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Introduction: resistance to carbapenem is widespread among Acinetobacter baumannii (A. baumannii) strains. Metallo-beta lactamases enzymes (MBL) are responsible for carbapenem resistance, as are oxacillinases (OXA). In recent years, MBL producing carbapenem-resistant strains have been reported in the world and Morocco at increasing rates. Our study aimed to investigate the presence of carbapenemases in acinetobacter strains isolated from hospitalized patients in CHU Fez.

Methods: a total of 58 imipenem-resistant A. baumannii strains isolated from clinical samples were investigated. The presence of MBL was described phenotypically by the double-disk synergy test (DDST), MBL E-test, and modified Hodge test. The bla IMP , bla VIM , genes, and bla OXA-23 , bla OXA-51 genes were investigated by multiplex polymerase chain reaction (PCR). The blaNDM-1 gene was determined by simplex PCR.

Results: fifty-eight strains were resistant to imipenem (98%), the modified Hodge test (MHT) was positive for 58 strains (100%), 47 strains (82%) were found to be positive for MBL by the test of double-disk synergy (DDST), 58 strains (100%) were positive by E-test MBL. The OXA 51 gene was detected in all strains, and the OXA 23 gene was detected in 53 strains (91%). In addition, the MBL genes were not detected by genotypic methods.

Conclusion: the OXA-23 and OXA-51 carbapenemases type are responsible for the resistance to carbapenems in A. baumannii resistant to carbapenems in our establishment. Resistance to carbapenems by MBL enzymes has been found by phenotypic tests, which must be confirmed by genotypic methods; and solicit other MBL genes.

Competing Interests: The authors declare no competing interests.

(Copyright: Abdelhamid Massik et al.)

Titel:	Detection of metallo-beta lactamases and oxacillinase genes in carbapenem-resistant Acinetobacter baumannii strains isolated in Morocco.
Autor/in / Beteiligte Person:	Massik, A ; Hibaoui, L ; Arhoune, B ; Yahyaoui, G ; Oumokhtar, B ; Mahmoud, M
Link:	Zum Volltext
Zeitschrift:	The Pan African medical journal, Jg. 40 (2021-12-07), S. 210
Veröffentlichung:	Kampala, Uganda : African Field Epidemiology Network, 2021
Medientyp:	academicJournal
ISSN:	1937-8688 (electronic)
DOI:	10.11604/pamj.2021.40.210.28663
Schlagwort:	Anti-Bacterial Agents pharmacology Bacterial Proteins genetics Carbapenems pharmacology Humans Microbial Sensitivity Tests Morocco beta-Lactamases genetics Acinetobacter baumannii genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Pan Afr Med J] 2021 Dec 07; Vol. 40, pp. 210. <i>Date of Electronic Publication: </i>2021 Dec 07 (<i>Print Publication: </i>2021). MeSH Terms: Acinetobacter baumannii* / genetics ; Anti-Bacterial Agents / pharmacology ; Bacterial Proteins / genetics ; Carbapenems / pharmacology ; Humans ; Microbial Sensitivity Tests ; Morocco ; beta-Lactamases / genetics References: Germs. 2019 Sep 02;9(3):133-141. (PMID: 31646143) ; Balkan Med J. 2015 Jan;32(1):79-83. (PMID: 25759776) ; J Pathog. 2016;2016:8603964. (PMID: 27123345) ; Clin Microbiol Infect. 2006 Sep;12(9):826-36. (PMID: 16882287) ; J Microbiol Immunol Infect. 2003 Mar;36(1):72-6. (PMID: 12741739) ; Chin Med J (Engl). 2009 Feb 5;122(3):301-6. (PMID: 19236808) ; J Antimicrob Chemother. 2007 Feb;59(2):321-2. (PMID: 17185300) ; Emerg Infect Dis. 2010 Jan;16(1):35-40. (PMID: 20031040) ; Int J Antimicrob Agents. 2009 May;33(5):405.e1-7. (PMID: 19095416) ; Lett Appl Microbiol. 1999 Jan;28(1):13-6. (PMID: 10030025) ; J Infect Dis. 2017 Feb 15;215(suppl_1):S28-S36. (PMID: 28375512) ; Clinics (Sao Paulo). 2010;65(9):825-9. (PMID: 21049207) ; Iran J Microbiol. 2020 Apr;12(2):107-112. (PMID: 32494344) ; Int J Antimicrob Agents. 2006 Apr;27(4):351-3. (PMID: 16564159) ; Emerg Infect Dis. 2011 Jan;17(1):129-30. (PMID: 21192874) ; Pan Afr Med J. 2016 Apr 15;23:191. (PMID: 27347280) ; Clin Microbiol Infect. 2001 Feb;7(2):88-91. (PMID: 11298149) ; Microb Drug Resist. 2016 Jul;22(5):412-31. (PMID: 26866778) ; J Clin Microbiol. 2008 Jun;46(6):2028-37. (PMID: 18322055) ; J Antimicrob Chemother. 2006 Mar;57(3):557-61. (PMID: 16431857) ; J Med Microbiol. 2008 Dec;57(Pt 12):1529-1532. (PMID: 19018025) ; Int J Microbiol. 2017;2017:8012104. (PMID: 28814955) ; Folia Histochem Cytobiol. 2016;54(2):61-74. (PMID: 27270503) ; J Clin Microbiol. 2002 Oct;40(10):3798-801. (PMID: 12354884) ; Ann Clin Microbiol Antimicrob. 2009 May 21;8:18. (PMID: 19460166) ; Antimicrob Agents Chemother. 2010 Mar;54(3):969-76. (PMID: 19995920) ; Int J Infect Dis. 2008 Jan;12(1):16-21. (PMID: 17513154) Contributed Indexing: Keywords: Acinetobacter baumannii; metallo-beta lactamases; oxacillinases Substance Nomenclature: 0 (Anti-Bacterial Agents) ; 0 (Bacterial Proteins) ; 0 (Carbapenems) ; EC 3.5.2.6 (beta-Lactamases) ; EC 3.5.2.6 (oxacillinase) Entry Date(s): Date Created: 20220209 Date Completed: 20220210 Latest Revision: 20220501 Update Code: 20231215 PubMed Central ID: PMC8783299

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Detection of metallo-beta lactamases and oxacillinase genes in carbapenem-resistant Acinetobacter baumannii strains isolated in Morocco.