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Background: MicroRNA plays an important role in multifarious biological processes by regulating their corresponding target genes. However, the biological function and regulatory mechanism of fungal microRNA-like RNAs (milRNAs) remain poorly understood.

Methods: In this study, combined with deep sequencing and bioinformatics analysis, milRNAs and their targets from Trichoderma guizhouence NJAU 4742 were isolated and identified under solid-state fermentation (SSF) by using rice straw as the sole carbon source at 28 °C and 37 °C, respectively.

Results: A critical milRNA, TGA1_S04_31828 ( Tr -milRNA1), was highly expressed under heat stress (37 °C) and adaptively regulated lignocellulase secretion. Overexpression of Tr -milRNA1 (OE- Tr -milRNA1) did not affect vegetative growth, but significantly increased lignocellulose utilization under heat stress. Based on the bioinformatics analysis and qPCR validation, a target of Tr -milRNA1 was identified as Trvip 36, a lectin-type cargo receptor. The expression of Tr -milRNA1 and Trvip36 showed a divergent trend under SSF when the temperature was increased from 28 °C to 37 °C. In addition, the expression of Trvip 36 was suppressed significantly in Tr -milRNA1 overexpression strain (OE- Tr -milRNA1). Compared with the wild type, deletion of Trvip 36 (Δ Trvip 36) significantly improved the secretion of lignocellulases by reducing the retention of lignocellulases in the ER under heat stress.

Conclusions: Tr -milRNA1 from NJAU 4742 improved lignocellulose utilization under heat stress by regulating the expression of the corresponding target gene Trvip 36. These findings might open avenues for exploring the mechanism of lignocellulase secretion in filamentous fungi.

Titel:	Tr -milRNA1 Contributes to Lignocellulase Secretion under Heat Stress by Regulating the Lectin-Type Cargo Receptor Gene Trvip36 in Trichoderma guizhouence NJAU 4742.
Autor/in / Beteiligte Person:	Li, T ; Liu, J ; Wang, Q ; Liu, Y ; Liu, D ; Shen, Q
Link:	Zum Volltext
Zeitschrift:	Journal of fungi (Basel, Switzerland), Jg. 7 (2021-11-23), Heft 12
Veröffentlichung:	Basel, Switzerland : MDPI AG, [2015]-, 2021
Medientyp:	academicJournal
ISSN:	2309-608X (electronic)
DOI:	10.3390/jof7120997
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Fungi (Basel)] 2021 Nov 23; Vol. 7 (12). <i>Date of Electronic Publication: </i>2021 Nov 23. References: Plant J. 2017 May;90(4):654-670. (PMID: 27943457) ; Annu Rev Phytopathol. 2010;48:225-46. (PMID: 20687832) ; Nat Rev Mol Cell Biol. 2010 Nov;11(11):777-88. (PMID: 20944667) ; Curr Protoc Bioinformatics. 2011 Dec;Chapter 12:Unit 12.10. (PMID: 22161567) ; Nat Genet. 2006 Jun;38 Suppl:S31-6. (PMID: 16736022) ; AMB Express. 2018 Mar 21;8(1):44. (PMID: 29564574) ; Bioinformatics. 2005 Apr 15;21(8):1401-2. (PMID: 15598838) ; Plant J. 2013 Jun;74(5):840-51. (PMID: 23480361) ; Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7. (PMID: 21037258) ; Plant Methods. 2007 Oct 12;3:12. (PMID: 17931426) ; Cell. 2009 Feb 20;136(4):642-55. (PMID: 19239886) ; Med Mycol. 2005 May;43 Suppl 1:S87-93. (PMID: 16110798) ; Nat Rev Genet. 2013 Feb;14(2):100-12. (PMID: 23329111) ; Fungal Genet Biol. 2020 Mar;136:103313. (PMID: 31751775) ; Mol Plant Pathol. 2021 Feb;22(2):243-254. (PMID: 33278058) ; New Phytol. 2020 Aug;227(3):899-913. (PMID: 32222083) ; Cell Mol Life Sci. 2010 Nov;67(22):3849-63. (PMID: 20680389) ; Mol Cell. 2010 Jun 25;38(6):803-14. (PMID: 20417140) ; Bioresour Technol. 2014 Apr;158:149-55. (PMID: 24589383) ; FEBS Lett. 2005 Oct 31;579(26):5872-8. (PMID: 16223485) ; J Histochem Cytochem. 2003 Aug;51(8):1057-63. (PMID: 12871987) ; Nat Plants. 2016 Sep 26;2(10):16153. (PMID: 27668926) ; Glycobiology. 2007 Sep;17(9):913-21. (PMID: 17586539) ; Mol Microbiol. 1992 Nov;6(22):3343-53. (PMID: 1484489) ; Environ Microbiol. 2019 Aug;21(8):2644-2658. (PMID: 30815928) ; Science. 2013 Oct 4;342(6154):118-23. (PMID: 24092744) ; Nucleic Acids Res. 2011 Jul;39(Web Server issue):W155-9. (PMID: 21622958) ; Biotechnol Biofuels. 2019 Jan 4;12:4. (PMID: 30622646) ; PLoS One. 2014 Aug 20;9(8):e104956. (PMID: 25141304) ; Microbiol Mol Biol Rev. 2012 Jun;76(2):115-58. (PMID: 22688810) ; J Appl Physiol (1985). 2002 May;92(5):2177-86. (PMID: 11960972) ; Philos Trans R Soc Lond B Biol Sci. 2019 Mar 4;374(1767):20180309. (PMID: 30967013) ; Annu Rev Microbiol. 2012;66:305-23. (PMID: 22746336) ; Traffic. 2010 Aug;11(8):1044-55. (PMID: 20477988) ; Nat Rev Microbiol. 2011 Sep 16;9(10):749-59. (PMID: 21921934) ; Autophagy. 2015;11(10):1849-63. (PMID: 25893854) ; Plant J. 2014 Dec;80(6):1108-17. (PMID: 25345491) ; Biotechnol Biofuels. 2015 Dec 24;8:228. (PMID: 26705422) ; Exp Cell Res. 2003 Aug 1;288(1):70-83. (PMID: 12878160) ; Fungal Biol. 2012 Nov;116(11):1156-62. (PMID: 23153806) ; Nat Rev Genet. 2015 Feb;16(2):71-84. (PMID: 25554358) ; PLoS One. 2013 Oct 02;8(10):e76288. (PMID: 24098464) ; Biotechnol Biofuels. 2013 Oct 16;6(1):149. (PMID: 24131596) ; Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438) ; Appl Environ Microbiol. 2015 Jan;81(2):533-43. (PMID: 25362068) ; Mol Cell. 2010 Oct 22;40(2):238-52. (PMID: 20965419) ; Glycobiology. 2004 Aug;14(8):739-44. (PMID: 15070860) ; Eukaryot Cell. 2012 Nov;11(11):1324-32. (PMID: 22822234) ; J Biol Chem. 2002 May 3;277(18):16332-9. (PMID: 11872745) Grant Information: Dongyang Liu (2018YFD0500201) National Key R & D Program of China; Dongyang Liu (31972513) National Natural Science Foundation of China; Dongyang Liu (KYZ201716) the Fundamental Research Funds for the Central Universities Contributed Indexing: Keywords: Trichoderma guizhouence NJAU 4742; heat stress; lignocellulase secretion; milRNA; posttranscriptional regulation Entry Date(s): Date Created: 20211224 Latest Revision: 20211229 Update Code: 20240513 PubMed Central ID: PMC8704016

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Tr -milRNA1 Contributes to Lignocellulase Secretion under Heat Stress by Regulating the Lectin-Type Cargo Receptor Gene Trvip36 in Trichoderma guizhouence NJAU 4742.