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Therapeutic efficacy of antineoplastic agents possessing a selective target to the nucleus of the cancer cells could be enhanced through novel formulation approaches. Thus, towards improvement of anticancer potential of icariin (ICA) on pancreatic cancer, the drug was entrapped into the polymeric poly lactic-co-glycolic acid (PLGA) with polyethylene glycol (PEG) as diblock copolymer. Optimization of the formulation was done using Statgraphics software to standardize percentages of PEG-PLGA and tween 80 (TW80) to obtain the smallest particle size. The optimized formulation was found to be in nanometer size and low PDI (0.321). Optimized formula enhanced cytotoxicity and apoptotic potential, compared with ICA-raw, against pancreatic cancer cell lines (aspc-1). The entrapment efficiency of the polymeric micelles was 72.34 ± 2.3% with 93.1 ± 6.5% release of ICA within 72 h. There was a twofold increase in apoptosis and sevenfold increase in necrosis of aspc-1 cells when incubated with raw ICA compared to control cells. Further, loss of mitochondrial membrane potential (⁓50-fold) by the ICA-loaded PMs and free drug compared to control cells was found to be due to the generation of ROS. Findings of cell cycle analysis revealed the significant arrest of G2-M phase of aspc-1 cells when incubated with the optimized formulation. Simultaneously, a significantly increased number of cells in pre-G1 revealed maximum apoptotic potential of the drug when delivered via micellar formulation. Finally, upregulation of caspase-3 established the superiority of the PMs approach against pancreatic cancer. In summary, the acquired results highlighted the potentiality of PMs delivery tool for controlling the growth of pancreatic cancer cells for improved efficacy.

Titel:	Development and Evaluation of Icariin-Loaded PLGA-PEG Nanoparticles for Potentiation the Proapoptotic Activity in Pancreatic Cancer Cells.
Autor/in / Beteiligte Person:	Alhakamy, NA
Link:	Zum Volltext
Zeitschrift:	AAPS PharmSciTech, Jg. 22 (2021-10-19), Heft 8, S. 252
Veröffentlichung:	New York : Springer ; <i>Original Publication</i>: Arlington, VA : American Association of Pharmaceutical Scientists, c2000-, 2021
Medientyp:	academicJournal
ISSN:	1530-9932 (electronic)
DOI:	10.1208/s12249-021-02111-w
Schlagwort:	Cell Line, Tumor Drug Carriers therapeutic use Flavonoids Humans Particle Size Polyethylene Glycols Nanoparticles Pancreatic Neoplasms drug therapy
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Retracted Publication Language: English [AAPS PharmSciTech] 2021 Oct 19; Vol. 22 (8), pp. 252. <i>Date of Electronic Publication: </i>2021 Oct 19. MeSH Terms: Nanoparticles* ; Pancreatic Neoplasms* / drug therapy ; Cell Line, Tumor ; Drug Carriers / therapeutic use ; Flavonoids ; Humans ; Particle Size ; Polyethylene Glycols Comments: Retraction in: AAPS PharmSciTech. 2023 Jul 19;24(6):153. (PMID: 37466739) References: Pancreatic cancer (PC) is the seventh leading cause... - Google Scholar [Internet]. [cited 2021 Apr 7]. Available from: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Pancreatic+cancer+%28PC%29+is+the+seventh+leading+cause+of+cancer-related+deaths+worldwide+with+432%2C242+related+deaths+in+2018+&btnG= . ; Morgell A, Reisz JA, Ateeb Z, Davanian H, Reinsbach SE, Halimi A, et al. 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Physically optimized nano-lipid carriers augment raloxifene and vitamin D oral bioavailability in healthy humans for management of osteoporosis. J Pharm Sci. 2020. Contributed Indexing: Keywords: cell cycle analysis; molecular markers; novel dosage form; nutraceutical; pancreatic cancer; polymeric micelles Substance Nomenclature: 0 (Drug Carriers) ; 0 (Flavonoids) ; 3WJQ0SDW1A (Polyethylene Glycols) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20211020 Date Completed: 20211125 Latest Revision: 20230719 Update Code: 20240513

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Development and Evaluation of Icariin-Loaded PLGA-PEG Nanoparticles for Potentiation the Proapoptotic Activity in Pancreatic Cancer Cells.