Protective effects of dietary icariin on lipopolysaccharide-induced acute oxidative stress and hepatopancreas injury in Chinese mitten crab, Eriocheir sinensis.
In: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, Jg. 251 (2022), S. 109192
Online
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Zugriff:
To investigate the effects of dietary icariin (ICA) supplementation on acute oxidative stress and hepatopancreatic injury induced by lipopolysaccharide (LPS) injection in Eriocheir sinensis, an 8-week feeding trial of crabs was conducted using 4 diets with different supplementation levels of ICA (0, 50, 100, and 200 mg/kg diet weight, respectively), and then challenged with LPS of 400 μg/kg body weight for 6 h. Results showed that 100 mg/kg ICA supplementation increased the antioxidant capacity, reduced the stress-related indicators in haemolymph, strengthen the mitochondrial membrane potential, and reduce apoptosis compared to the single LPS-treated crabs. The expressions of apoptosis-related genes and proteins were also evaluated to further understand the effects of dietary ICA pretreatment on LPS-induced cell apoptosis. As a result, dietary 100 mg/kg diet weight ICA pre-addition significantly down-regulated the expression of HSP60, HSP70, Caspase 3c, Caspase 8, Caspase 3, Caspase 9, P38, and Bax (P < 0.05), and alleviated the suppressed expression of PI3K, AKT, MEK, and Bcl-2 (P < 0.05) in crabs challenged with LPS. Overall, this research reveals that ICA supplementation of 100 mg/kg diet weight could enhance the resistance to oxidative damage and apoptosis in E. sinensis facing LPS challenge.
(Copyright © 2021. Published by Elsevier Inc.)
Titel: |
Protective effects of dietary icariin on lipopolysaccharide-induced acute oxidative stress and hepatopancreas injury in Chinese mitten crab, Eriocheir sinensis.
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Autor/in / Beteiligte Person: | Zheng, X ; Jiang, W ; Zhang, L ; Abasubong, KP ; Zhang, D ; Li, X ; Jiang, G ; Chi, C ; Liu, W |
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Zeitschrift: | Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, Jg. 251 (2022), S. 109192 |
Veröffentlichung: | New York, NY : Elsevier Science, c2000-, 2022 |
Medientyp: | academicJournal |
ISSN: | 1532-0456 (print) |
DOI: | 10.1016/j.cbpc.2021.109192 |
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