Imine reduction by an Ornithine cyclodeaminase/μ-crystallin homolog purified from Candida parapsilosis ATCC 7330.
In: Biotechnology reports (Amsterdam, Netherlands), Jg. 31 (2021-08-05), S. e00664
Online
academicJournal
Zugriff:
We report a stereospecific imine reductase from Candida parapsilosis ATCC 7330 (CpIM1), a versatile biocatalyst and a rich source of highly stereospecific oxidoreductases. The recombinant gene was overexpressed in Escherichia coli and the protein CpIM1 was purified to homogeneity. This protein belongs to the Ornithine cyclodeaminase/ μ-crystallin (OCD-Mu) family of proteins which has only a few characterized members. CpIM1 catalyzed the alkylamination of α-keto acids/esters producing exclusively ( S )- N -alkyl amino acids/esters e.g. N- methyl-l-alanine with > 90% conversion and > 99% enantiomeric excess (ee). The enzyme showed the highest activity for the alkylamination of pyruvate and methylamine leading to N- methyl-l-alanine with an apparent K M of 15.04 ± 2.8 mM and V max of 13.75 ± 1.07 μmol/min/mg. CpIM1 also catalyzed (i) the reduction of imines e.g. 2-methyl-1-pyrroline to ( S )-2-methylpyrrolidine with ∼30% conversion and 75% ee and (ii) the dehydrogenation of cyclic amino acids e.g. l-Proline (as monitered by reduction of cofactor NADP + spectrophotometrically).
Competing Interests: The authors whose names are listed immediately below certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.
(© 2021 The Authors. Published by Elsevier B.V.)
Titel: |
Imine reduction by an Ornithine cyclodeaminase/μ-crystallin homolog purified from Candida parapsilosis ATCC 7330.
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Autor/in / Beteiligte Person: | Uma Mahesh, VNM ; Chadha, A |
Link: | |
Zeitschrift: | Biotechnology reports (Amsterdam, Netherlands), Jg. 31 (2021-08-05), S. e00664 |
Veröffentlichung: | [Amsterdam] : Elsevier B. V., [2014]-, 2021 |
Medientyp: | academicJournal |
ISSN: | 2215-017X (print) |
DOI: | 10.1016/j.btre.2021.e00664 |
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