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Within the plant and Earth sciences, stable isotope analysis is a versatile tool conveying information (inter alia) about plant physiological and paleoclimate variability across scales. Here, we identify a 13C signal (i.e. systematic 13C/12C variation) at tree-ring glucose C-4 and report an experimentally testable theory on its origin. We propose the signal is introduced by glyceraldehyde-3-phosphate dehydrogenases in the cytosol of leaves. It conveys two kinds of (potentially convoluted) information: (i) commitment of glyceraldehyde 3-phosphate to 3-phosphoglycerate versus fructose 1,6-bisphosphate metabolism; and (ii) the contribution of non-phosphorylating versus phosphorylating glyceraldehyde-3-phosphate dehydrogenase to catalysing the glyceraldehyde 3-phosphate to 3-phosphoglycerate forward reaction of glycolysis. The theory is supported by 13C fractionation modelling. Modelling results provide the first evidence in support of the cytosolic oxidation-reduction (COR) cycle, a carbon-neutral mechanism supplying NADPH at the expense of ATP and NADH, which may help to maintain leaf-cytosolic redox balances. In line with expectations related to COR cycling, we found a positive correlation between air vapour pressure deficit and 13C discrimination at glucose C-4. Overall, 13C-4 signal analysis may enable an improved understanding of leaf carbon and energy metabolism.

Titel:	Carbon flux around leaf-cytosolic glyceraldehyde-3-phosphate dehydrogenase introduces a 13C signal in plant glucose.
Autor/in / Beteiligte Person:	Wieloch, T ; Werner, RA ; Schleucher, J
Link:	Zum Volltext
Zeitschrift:	Journal of experimental botany, Jg. 72 (2021-10-26), Heft 20, S. 7136-7144
Veröffentlichung:	Oxford, UK : Oxford University Press,, 2021
Medientyp:	academicJournal
ISSN:	1460-2431 (electronic)
DOI:	10.1093/jxb/erab316
Schlagwort:	Carbon Cycle Carbon Isotopes metabolism Cytosol metabolism Glycolysis Kinetics Plant Leaves metabolism Glucose metabolism Glyceraldehyde-3-Phosphate Dehydrogenases metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Exp Bot] 2021 Oct 26; Vol. 72 (20), pp. 7136-7144. MeSH Terms: Glucose* / metabolism ; Glyceraldehyde-3-Phosphate Dehydrogenases* / metabolism ; Carbon Cycle ; Carbon Isotopes / metabolism ; Cytosol / metabolism ; Glycolysis ; Kinetics ; Plant Leaves / metabolism References: Oecologia. 2015 Nov;179(3):641-54. (PMID: 26130023) ; Ann Bot. 2002 Jun;89 Spec No:841-50. (PMID: 12102510) ; Biochem J. 2011 May 15;436(1):15-34. (PMID: 21524275) ; FEBS Lett. 1994 Feb 14;339(1-2):97-100. (PMID: 8313985) ; J Plant Physiol. 2011 Jan 15;168(2):79-87. (PMID: 20800930) ; Biochim Biophys Acta. 1990 Nov 9;1036(2):138-42. (PMID: 2223831) ; Isotopes Environ Health Stud. 2015;51(1):155-99. (PMID: 25894429) ; Plant Physiol. 1973 Aug;52(2):111-8. (PMID: 16658509) ; J Exp Bot. 2021 May 28;72(12):4186-4189. (PMID: 33739373) ; Plant Signal Behav. 2008 Mar;3(3):156-65. (PMID: 19513210) ; Curr Opin Plant Biol. 2003 Jun;6(3):236-46. (PMID: 12753973) ; Biochemistry. 1991 Sep 10;30(36):8871-6. (PMID: 1888744) ; Plant Sci. 2000 May 29;154(2):107-115. (PMID: 10729609) ; J Mol Biol. 1994 Mar 18;237(1):165-71. (PMID: 7545914) ; Plant Physiol. 1990 Nov;94(3):1337-44. (PMID: 16667838) ; Arch Biochem Biophys. 1988 Feb 1;260(2):830-40. (PMID: 3341766) ; Proc Natl Acad Sci U S A. 1961 May 15;47:623-32. (PMID: 13681011) ; Sci Rep. 2018 Mar 22;8(1):5048. (PMID: 29567963) ; Int J Mol Sci. 2013 Apr 12;14(4):8073-92. (PMID: 23584025) ; Plant Signal Behav. 2012 Jul;7(7):767-70. (PMID: 22751307) ; J Biol Chem. 1997 Feb 28;272(9):5382-7. (PMID: 9038136) Contributed Indexing: Keywords: Carbon allocation; carbon flux; carbon stable isotopes; cytosolic oxidation–reduction cycle; energy metabolism; glyceraldehyde-3-phosphate dehydrogenase; intramolecular isotope analysis; isotope fractionation model; primary carbon metabolism Substance Nomenclature: 0 (Carbon Isotopes) ; EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases) ; FDJ0A8596D (Carbon-13) ; IY9XDZ35W2 (Glucose) Entry Date(s): Date Created: 20210705 Date Completed: 20211124 Latest Revision: 20211124 Update Code: 20231215 PubMed Central ID: PMC8547152

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Carbon flux around leaf-cytosolic glyceraldehyde-3-phosphate dehydrogenase introduces a 13C signal in plant glucose.