Radiation- and Photo-Induced Oxidation Pathways of Methionine in Model Peptide Backbone under Anoxic Conditions.
In: International journal of molecular sciences, Jg. 22 (2021-04-30), Heft 9
Online
academicJournal
Zugriff:
Within the reactive oxygen species (ROS) generated by cellular metabolisms, hydroxyl radicals (HO • ) play an important role, being the most aggressive towards biomolecules. The reactions of HO • with methionine residues (Met) in peptides and proteins have been intensively studied, but some fundamental aspects remain unsolved. In the present study we examined the biomimetic model made of Ac-Met-OMe, as the simplest model peptide backbone, and of HO • generated by ionizing radiation in aqueous solutions under anoxic conditions. We performed the identification and quantification of transient species by pulse radiolysis and of final products by LC-MS and high-resolution MS/MS after γ-radiolysis. By parallel photochemical experiments, using 3-carboxybenzophenone (CB) triplet with the model peptide, we compared the outcomes in terms of short-lived intermediates and stable product identification. The result is a detailed mechanistic scheme of Met oxidation by HO • , and by CB triplets allowed for assigning transient species to the pathways of products formation.
Titel: |
Radiation- and Photo-Induced Oxidation Pathways of Methionine in Model Peptide Backbone under Anoxic Conditions.
|
---|---|
Autor/in / Beteiligte Person: | Pędzinski, T ; Grzyb, K ; Skotnicki, K ; Filipiak, P ; Bobrowski, K ; Chatgilialoglu, C ; Marciniak, B |
Link: | |
Zeitschrift: | International journal of molecular sciences, Jg. 22 (2021-04-30), Heft 9 |
Veröffentlichung: | Basel, Switzerland : MDPI, [2000-, 2021 |
Medientyp: | academicJournal |
ISSN: | 1422-0067 (electronic) |
DOI: | 10.3390/ijms22094773 |
Schlagwort: |
|
Sonstiges: |
|