Einzeltreffer — DigiBib

Background: Tissue engineering technology has been applied extensively for clinical research and human amnion mesenchymal stem cells (hAMSCs) could cause mesenchymal stem cells to differentiate into the bone tissue. However, it is necessary to develop and identify the safer appropriate amount of osteogenic inducer. The objective of this study is to investigate the effect of icariin (ICA) on the proliferation and osteogenic differentiation of hAMSCs.

Methods: The morphology and phenotype of hAMSCs were discovered by flow cytometry and immunocytochemical staining. The osteogenic differentiation of hAMSCs under the influence of different concentrations of ICA were assessed by alkaline phosphatase (ALP) activity substrate assay and alizarin red staining.

Results: MTT assay revealed that the hAMSCs pretreated with ICA exhibited increased proliferation when compared with the control group, and the most optimum concentration of ICA was 1 × 10 - 6 mol/L. The combined analysis of ALP activity and ARS staining showed that ICA could significantly promote the osteogenic differentiation of hAMSCs, and the effect was most significant when the concentration of ICA was 1 × 10 - 6 mol/L.

Conclusion: All the above results implied that ICA could significantly increase proliferation and enhance the osteogenic differentiation of hAMSCs, especially when the concentration of ICA was 1 × 10 - 6 mol/L.

Titel:	Effects of icariin on the proliferation and osteogenic differentiation of human amniotic mesenchymal stem cells.
Autor/in / Beteiligte Person:	Wang, F ; Yang, Z ; He, W ; Song, Q ; Wang, K ; Zhou, Y
Link:	Zum Volltext
Zeitschrift:	Journal of orthopaedic surgery and research, Jg. 15 (2020-12-02), Heft 1, S. 578
Veröffentlichung:	London : BioMed Central, 2006-, 2020
Medientyp:	academicJournal
ISSN:	1749-799X (electronic)
DOI:	10.1186/s13018-020-02076-9
Schlagwort:	Cells, Cultured Dose-Response Relationship, Drug Humans Mesenchymal Stem Cells drug effects Amnion cytology Bone and Bones Cell Differentiation drug effects Cell Proliferation drug effects Flavonoids pharmacology Mesenchymal Stem Cells physiology Osteogenesis drug effects Tissue Engineering methods
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Orthop Surg Res] 2020 Dec 02; Vol. 15 (1), pp. 578. <i>Date of Electronic Publication: </i>2020 Dec 02. MeSH Terms: Bone and Bones* ; Amnion / cytology ; Cell Differentiation / drug effects ; Cell Proliferation / drug effects ; Flavonoids / pharmacology ; Mesenchymal Stem Cells / physiology ; Osteogenesis / drug effects ; Tissue Engineering / *methods ; Cells, Cultured ; Dose-Response Relationship, Drug ; Humans ; Mesenchymal Stem Cells / drug effects References: Evid Based Complement Alternat Med. 2017;2017:8085325. (PMID: 29445413) ; Exp Cell Res. 2016 Jul 15;345(2):218-29. (PMID: 27237096) ; Biomed Pharmacother. 2020 Mar;123:109807. (PMID: 31896066) ; IUBMB Life. 2020 Feb;72(2):296-304. (PMID: 31509344) ; Musculoskelet Surg. 2015 Dec;99(3):171-8. (PMID: 25845670) ; Ren Fail. 1997 May;19(3):409-24. (PMID: 9154658) ; Int J Clin Exp Pathol. 2015 May 01;8(5):4367-77. (PMID: 26191128) ; Theranostics. 2020 May 15;10(14):6216-6230. (PMID: 32483449) ; Am J Transl Res. 2017 May 15;9(5):2077-2087. (PMID: 28559962) ; Stem Cell Rev Rep. 2015 Aug;11(4):570-85. (PMID: 25381565) ; Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):694-699. (PMID: 28871695) ; BMC Complement Altern Med. 2019 Jul 3;19(1):155. (PMID: 31269947) ; Menopause. 2012 Feb;19(2):225-33. (PMID: 22011754) ; Biotechnol Adv. 2015 Dec;33(8):1626-40. (PMID: 26341834) ; J Tradit Chin Med. 2016 Jun;36(3):360-8. (PMID: 27468552) ; Curr Pharm Des. 2013;19(13):2459-73. (PMID: 23278600) ; Sci Rep. 2016 May 04;6:25282. (PMID: 27141994) ; Int J Clin Exp Med. 2015 May 15;8(5):7125-33. (PMID: 26221250) ; Phytother Res. 2014 Jan;28(1):1-8. (PMID: 23494901) ; Pharmazie. 2005 Dec;60(12):939-42. (PMID: 16398272) ; Osteoarthritis Cartilage. 2012 Dec;20(12):1647-56. (PMID: 22917745) ; Cell Transplant. 2017 Apr 13;26(4):531-539. (PMID: 27938500) ; Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13726-31. (PMID: 9811868) ; J Asian Nat Prod Res. 2006 Oct-Nov;8(7):649-56. (PMID: 17135052) ; J Cell Biochem. 2019 Aug;120(8):13121-13132. (PMID: 30887562) ; J Cell Mol Med. 2018 Mar;22(3):1428-1442. (PMID: 29392844) ; Science. 1999 Dec 3;286(5446):1946-9. (PMID: 10583956) ; J Bone Miner Res. 1994 Mar;9(3):395-400. (PMID: 8191934) ; J Periodontol. 2018 May;89(5):596-605. (PMID: 29520784) ; J Pharmacol Exp Ther. 1999 Sep;290(3):1054-64. (PMID: 10454478) ; Br J Ophthalmol. 1999 Apr;83(4):399-402. (PMID: 10434859) ; J Ethnopharmacol. 2011 Mar 24;134(2):268-74. (PMID: 21167926) ; Calcif Tissue Int. 1997;61 Suppl 1:S12-4. (PMID: 9263610) ; Molecules. 2011 Dec 06;16(12):10123-33. (PMID: 22146373) ; Eur J Pharmacol. 2013 Aug 15;714(1-3):15-22. (PMID: 23764463) ; Acta Histochem. 2018 Nov;120(8):701-712. (PMID: 30078494) ; Biochem Biophys Res Commun. 2008 May 2;369(2):444-8. (PMID: 18295595) ; Am J Obstet Gynecol. 1960 Jun;79:1070-3. (PMID: 13803349) Grant Information: QKHJZ-LKZ[2013]29.g Guizhou Science and Technology Department Contributed Indexing: Keywords: Human amniotic mesenchymal stem cell; Icariin; Osteogenic differentiation; Proliferation Substance Nomenclature: 0 (Flavonoids) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20201203 Date Completed: 20210617 Latest Revision: 20210617 Update Code: 20240513 PubMed Central ID: PMC7709318

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Effects of icariin on the proliferation and osteogenic differentiation of human amniotic mesenchymal stem cells.