Einzeltreffer — DigiBib

Treatment with mesenchymal stem cells (MSCs) has been revealed to suppress CD4 + T cells and autoimmunity in both mouse models and patients with primary Sjögren syndrome (pSS); however, the underlying mechanism remains unclear. MicroRNAs (miRNAs or miRs) mediate CD4 + T cell activation, but the mechanism is not understood, particularly for CD4 + T cells treated with MSCs. Characterization of miRNAs may reveal pSS pathogenesis, guide MSC treatment and provide more personalized management options. The present study aimed to perform an miRNome analysis of quiescent and T cell receptor (TCR)‑activated CD4 + T cells treated with MSCs via miRNA proﬁles and bioinformatics. Following 72 h of co‑culture, MSCs inhibited TCR‑induced CD4 + T cell activation and decreased IFN‑γ levels. The numbers of aberrant miRNAs in pSS naïve (vs. healthy naïve), pSS activation (vs. pSS naïve), MSC treatment and pre‑IFN‑γ MSC treatment (vs. pSS activation) groups were 42, 55, 27 and 32, respectively. Gene enrichment analysis revealed that 259 pathways were associated with CD4 + T cell stimulation, and 240 pathways were associated with MSC treatment. Increased miRNA‑7150 and miRNA‑5096 and decreased miRNA‑125b‑5p and miRNA‑22‑3p levels in activated CD4 + T cells from patients with pSS were reversed by MSC treatment. Notably, the proliferation of CD4 + T cells and CD4 + IFN‑γ + cells, expression levels of miRNA‑125b‑5p and miRNA‑155 in CD4 + T cells and supernatant IFN‑γ secretion were associated with disease activity. miRNA may play a vital role in MSC treatment for activated CD4 + T cells. The results indicated that the expression levels of miRNA‑125b‑5p and miRNA‑155 in TCR‑activated CD4 + T cells from patients with pSS may provide insight regarding autoimmune diseases and offer a novel target for prospective treatment. Therefore, these results may be crucial in providing MSC treatment for pSS.

Titel:	Mesenchymal stem cells negatively regulate CD4 <superscript>+</superscript> T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.
Autor/in / Beteiligte Person:	Gong, B ; Zheng, L ; Lu, Z ; Huang, J ; Pu, J ; Pan, S ; Zhang, M ; Liu, J ; Tang, J
Zeitschrift:	Molecular medicine reports, Jg. 23 (2021), Heft 1
Veröffentlichung:	Athens, Greece : D. A. Spandidos, 2021
Medientyp:	academicJournal
ISSN:	1791-3004 (electronic)
DOI:	10.3892/mmr.2020.11681
Schlagwort:	Adult Aged CD4-Positive T-Lymphocytes immunology Case-Control Studies Cells, Cultured Coculture Techniques Female Gene Expression Profiling Humans Interferon-gamma metabolism Lymphocyte Activation Male Mesenchymal Stem Cells immunology Middle Aged Receptors, Antigen, T-Cell metabolism Signal Transduction Sjogren's Syndrome immunology Young Adult CD4-Positive T-Lymphocytes cytology Gene Regulatory Networks Mesenchymal Stem Cells cytology MicroRNAs genetics Sjogren's Syndrome genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Mol Med Rep] 2021 Jan; Vol. 23 (1). <i>Date of Electronic Publication: </i>2020 Nov 12. MeSH Terms: Gene Regulatory Networks* ; CD4-Positive T-Lymphocytes / cytology ; Mesenchymal Stem Cells / cytology ; MicroRNAs / genetics ; Sjogren's Syndrome / genetics ; Adult ; Aged ; CD4-Positive T-Lymphocytes / immunology ; Case-Control Studies ; Cells, Cultured ; Coculture Techniques ; Female ; Gene Expression Profiling ; Humans ; Interferon-gamma / metabolism ; Lymphocyte Activation ; Male ; Mesenchymal Stem Cells / immunology ; Middle Aged ; Receptors, Antigen, T-Cell / metabolism ; Signal Transduction ; Sjogren's Syndrome / immunology ; Young Adult References: Genome Res. 2007 Oct;17(10):1537-45. (PMID: 17785539) ; Gastroenterology. 2008 Mar;134(3):833-48. (PMID: 18243183) ; Genes Immun. 2014 Mar;15(2):115-25. (PMID: 24401767) ; J Immunol. 2015 Aug 15;195(4):1470-9. (PMID: 26163591) ; Expert Opin Pharmacother. 2013 Feb;14(3):279-89. (PMID: 23346917) ; Semin Immunopathol. 2013 Mar;35(2):139-50. (PMID: 23053397) ; Am J Physiol Lung Cell Mol Physiol. 2015 Jan 1;308(1):L86-95. (PMID: 25361568) ; Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11499-504. (PMID: 23798430) ; APMIS. 2001 May;109(5):345-55. (PMID: 11478682) ; Nature. 2008 Jul 3;454(7200):56-61. (PMID: 18548003) ; Arthritis Rheum. 2011 Feb;63(2):535-44. (PMID: 21280008) ; Anat Cell Biol. 2013 Dec;46(4):262-71. (PMID: 24386599) ; BMC Bioinformatics. 2006 Jan 19;7:30. (PMID: 16423281) ; Cytotherapy. 2004;6(4):372-9. (PMID: 16146890) ; Nat Immunol. 2014 Nov;15(11):1009-16. (PMID: 25329189) ; Clin Exp Immunol. 2007 Aug;149(2):353-63. (PMID: 17521318) ; J Immunol. 2011 Sep 1;187(5):2233-43. (PMID: 21788445) ; Nucleic Acids Res. 2020 Jan 8;48(D1):D498-D503. (PMID: 31691815) ; Eur J Immunol. 2014 Jan;44(1):11-5. (PMID: 24571026) ; Microrna. 2015;4(2):117-22. (PMID: 26290349) ; Nat Immunol. 2011 Jun 26;12(8):796-803. (PMID: 21706005) ; J Autoimmun. 2016 Apr;68:52-61. (PMID: 26786119) ; Stem Cells. 2006 Feb;24(2):386-98. (PMID: 16123384) ; N Engl J Med. 2018 Mar 08;378(10):931-939. (PMID: 29514034) ; Arthritis Res Ther. 2010;12(1):R31. (PMID: 20175883) ; J Immunol. 2013 Jun 1;190(11):5809-17. (PMID: 23630358) ; Ann Rheum Dis. 2002 Jun;61(6):554-8. (PMID: 12006334) ; Database (Oxford). 2016 Jul 03;2016:. (PMID: 27374120) ; Nucleic Acids Res. 2004 Jan 1;32(Database issue):D277-80. (PMID: 14681412) ; Nucleic Acids Res. 2019 Jan 8;47(D1):D330-D338. (PMID: 30395331) ; Bioinformatics. 2003 Jan 22;19(2):185-93. (PMID: 12538238) ; Blood. 2012 Oct 11;120(15):3142-51. (PMID: 22927248) ; Crit Rev Immunol. 2013;33(5):435-76. (PMID: 24099302) ; Front Immunol. 2019 Jun 04;10:1191. (PMID: 31214172) ; J Immunol. 2011 Dec 1;187(11):5834-41. (PMID: 22043014) ; Ann Rheum Dis. 2010 Jun;69(6):1103-9. (PMID: 19561361) ; Curr Mol Med. 2012 Jun;12(5):574-91. (PMID: 22515979) Contributed Indexing: Keywords: CD4+ T cell; mesenchymal stem cells; microRNA; primary Sjögren syndrome; T cell receptor pathway Substance Nomenclature: 0 (IFNG protein, human) ; 0 (MIRN125 microRNA, human) ; 0 (MIRN155 microRNA, human) ; 0 (MicroRNAs) ; 0 (Receptors, Antigen, T-Cell) ; 82115-62-6 (Interferon-gamma) Entry Date(s): Date Created: 20201112 Date Completed: 20210519 Latest Revision: 20221222 Update Code: 20240513 PubMed Central ID: PMC7684863

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Mesenchymal stem cells negatively regulate CD4 <superscript>+</superscript> T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.