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Icariin (ICA) has anti-inflammatory effects in some diseases, but its role in gouty arthritis (GA) is not clear. This study investigated the effects of ICA in monosodium urate (MSU)-induced GA rat models. GA rat models were induced by MSU, and co-treated with ICA of low-dose (20 mg/kg), medium-dose (40 mg/kg), and high-dose (80 mg/kg), respectively. The ankle swelling rates, haematoxylin-eosin (HE) staining changes, inflammatory factors (interleukin (IL)-1β, IL-6, tumour necrosis factor-α (TNF-α)) and prostaglandin E2 (PGE 2 ) levels in synovial tissues were detected. The antioxidants levels in rat serum, and NF-κB pathway-related proteins and NALP3 inflammasome expressions in synovial tissues were also analysed. In cell experiments, chondrocytes were co-treated with different concentrations of ICA (1, 5, 10 μmol/L) on the basis of MSU. The activities and inflammatory cytokines, hydroxyproline (Hyp) and glycosaminogly (GAG) expressions in chondrocytes were measured. In rat experiments, MUS increased the ankle swelling rates, promoted inflammatory cells infiltration, and increased IL-1β, IL-6, TNF-α, PGE 2 levels in synovial tissues, which were all alleviated by ICA. Moreover, ICA also suppressed nuclear translocation of NF-κB pathway-related proteins and reduced the expression of NALP3 inflammasome in rat models. As for cell experiments, ICA decreased the activity, inflammatory cytokines and GAG levels, and suppressed nuclear translocation of NF-κB pathway-related proteins of MSU-treated chondrocytes. In general, medium and high concentrations of ICA showed good effects. ICA has an inhibitory effect in MSU-induced rat GA models through NF-κB/NALP3 pathway, which may provide a direction for the treatment of GA. SIGNIFICANCE: Icariin (ICA) has anti-inflammatory effects in some diseases, but its role in gouty arthritis (GA) is not clear. This study excogitated that monosodium urate (MSU) increased the ankle swelling rates of rats, promoted inflammatory cells infiltration, and increased cytokines levels in synovial tissues, which were all alleviated by ICA. In related mechanism, we found that ICA might exert the catabatic functions through the NF-κB/NALP3 pathway. The findings of this study clarified that ICA may provide a direction for the treatment of patients with GA and illustrated the relevant underlying mechanism of its role.

Titel:	Icariin alleviates MSU-induced rat GA models through NF-κB/NALP3 pathway.
Autor/in / Beteiligte Person:	Cao, Y
Link:	Zum Volltext
Zeitschrift:	Cell biochemistry and function, Jg. 39 (2021-04-01), Heft 3, S. 357-366
Veröffentlichung:	Oxford, England : Wiley-Blackwell ; <i>Original Publication</i>: Guildford, Surrey : Butterworth Scientific Ltd., c1983-, 2021
Medientyp:	academicJournal
ISSN:	1099-0844 (electronic)
DOI:	10.1002/cbf.3598
Schlagwort:	Animals Cytokines metabolism Female Male Rats Rats, Sprague-Dawley Arthritis, Gouty chemically induced Arthritis, Gouty metabolism Arthritis, Gouty pathology Arthritis, Gouty prevention & control Flavonoids pharmacology NF-kappa B metabolism NLR Family, Pyrin Domain-Containing 3 Protein metabolism Signal Transduction drug effects Uric Acid toxicity
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Cell Biochem Funct] 2021 Apr; Vol. 39 (3), pp. 357-366. <i>Date of Electronic Publication: </i>2020 Nov 01. MeSH Terms: Arthritis, Gouty* / chemically induced ; Arthritis, Gouty* / metabolism ; Arthritis, Gouty* / pathology ; Arthritis, Gouty* / prevention & control ; Flavonoids / pharmacology ; NF-kappa B / metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein / metabolism ; Signal Transduction / drug effects ; Uric Acid / *toxicity ; Animals ; Cytokines / metabolism ; Female ; Male ; Rats ; Rats, Sprague-Dawley References: Yu KH, Chen DY, Chen JH, et al. Management of gout and hyperuricemia: multidisciplinary consensus in Taiwan. Int J Rheum Dis. 2018;21(4):772-787. ; Tausche AK, Aringer M. Gouty arthritis. Zeitschrift fur Rheumatologie. 2016;75(9):885-898. ; Lee YM, Son E, Kim SH, Kim OS, Kim DS. Anti-inflammatory and anti-osteoarthritis effect of Mollugo pentaphylla extract. Pharm Biol. 2019;57(1):74-81. ; Cleophas MC, Crisan TO, Joosten LA. Factors modulating the inflammatory response in acute gouty arthritis. Curr Opin Rheumatol. 2017;29(2):163-170. ; Wilson L, Saseen JJ. Gouty arthritis: a review of acute management and prevention. Pharmacotherapy. 2016;36(8):906-922. ; Bardin T, Voshaar MA, van de Laar MA. The human and economic burden of difficult-to-treat gouty arthritis. Joint Bone Spine revue du rhumatisme. 2015;82(Suppl 1):eS2-eS8. ; Fang J, Zhang Y. Icariin, an anti-atherosclerotic drug from Chinese medicinal herb horny goat weed. Front Pharmacol. 2017;8:734. ; Wang Z, Wang D, Yang D, Zhen W, Zhang J, Peng S. The effect of icariin on bone metabolism and its potential clinical application. Osteoporos Int. 2018;29(3):535-544. ; Jiang S, Chang H, Deng S, Fan D. Icariin enhances the chemosensitivity of cisplatinresistant ovarian cancer cells by suppressing autophagy via activation of the AKT/mTOR/ATG5 pathway. Int J Oncol. 2019;54(6):1933-1942. ; Angeloni C, Barbalace MC, Hrelia S. Icariin and its metabolites as potential protective phytochemicals against Alzheimer's disease. Front Pharmacol. 2019;10:271. ; Xu CQ, Le JJ, Duan XH, et al. Molecular mechanism of icariin on rat asthmatic model. Chin Med J. 2011;124(18):2899-2906. ; Zhang B, Wang G, He J, et al. Icariin attenuates neuroinflammation and exerts dopamine neuroprotection via an Nrf2-dependent manner. J Neuroinflamm. 2019;16(1):92. ; Huang H, Zhang ZF, Qin FW, et al. Icariin inhibits chondrocyte apoptosis and angiogenesis by regulating the TDP-43 signaling pathway. Mol Genet Genom Med. 2019;7(4):e00586. ; Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2^(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinf Biomath. 2013;3(3):71-85. ; Pavelka K. Treat to target in gouty arthritis. Vnitrni lekarstvi. 2018;64(2):185-190. ; Abdellatif AA, Elkhalili N. Management of gouty arthritis in patients with chronic kidney disease. Am J Therapeut. 2014;21(6):523-534. ; Vinik O, Wechalekar MD, Falzon L, Buchbinder R, van der Heijde DM, Bombardier C. Treatment of asymptomatic hyperuricemia for the prevention of gouty arthritis, renal disease, and cardiovascular events: a systematic literature review. J Rheumatol Suppl. 2014;92:70-74. ; Cronstein BN, Sunkureddi P. Mechanistic aspects of inflammation and clinical management of inflammation in acute gouty arthritis. J Clin Rheumatol Pract Rep Rheum Musculoskelet Dis. 2013;19(1):19-29. ; Reber LL, Marichal T, Sokolove J, et al. Contribution of mast cell-derived interleukin-1beta to uric acid crystal-induced acute arthritis in mice. Arthritis Rheumatol (Hoboken, NJ). 2014;66(10):2881-2891. ; Zhou GY, Guo JL, Li-Qi BI. Interleukin-6 levels in serum and synovial fluid in patients with arthritis. J Jilin Univ. 2004;30(5):772-774. ; Yokose K, Sato S, Asano T, et al. TNF-alpha potentiates uric acid-induced interleukin-1beta (IL-1beta) secretion in human neutrophils. Mod Rheumatol. 2018;28(3):513-517. ; Tseuguem PP, Nguelefack TB, Piégang BN, Mbankou Ngassam S. Aqueous and Methanol Extracts of Paullinia pinnata (Sapindaceae) Improve Monosodium Urate-Induced Gouty Arthritis in Rat: Analgesic, Anti-Inflammatory, and Antioxidant Effects. Evidence-based complementary and alternative medicine: eCAM. 2019;2019:5946291. https://doi.org/10.1155/2019/5946291. ; Deng X, Wu W, Liang H, et al. Icariin prevents IL-1beta-induced apoptosis in human nucleus pulposus via the PI3K/AKT pathway. Evid Based Complement Altern Med eCAM. 2017;2017:2198323. ; Hsieh TP, Sheu SY, Sun JS, Chen MH. Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-kappaB regulated HIF-1alpha and PGE(2) synthesis. Phytomed Int J Phytother Phytopharmacol. 2011;18(2-3):176-185. ; Hu Y, Sun B, Liu K, et al. Icariin attenuates high-cholesterol diet induced atherosclerosis in rats by inhibition of inflammatory response and p38 MAPK signaling pathway. Inflammation. 2016;39(1):228-236. ; Zhang Y, Wei Y, Zhu Z, et al. Icariin enhances radiosensitivity of colorectal cancer cells by suppressing NF-kappaB activity. Cell Biochem Biophys. 2014;69(2):303-310. ; Zhang QB, Qing YF, Yin CC, et al. Mice with miR-146a deficiency develop severe gouty arthritis via dysregulation of TRAF 6, IRAK 1 and NALP3 inflammasome. Arthritis Res Ther. 2018;20(1):45. ; Zhou Q, Yu DH, Zhang N, Liu SM. Anti-inflammatory effect of total saponin fraction from Dioscorea nipponica Makino on gouty arthritis and its influence on NALP3 inflammasome. Chin J Integr Med. 2019;25(9):663-670. ; Xu Y, Dai GJ, Liu Q, et al. Sanmiao formula inhibits chondrocyte apoptosis and cartilage matrix degradation in a rat model of osteoarthritis. Experimental and therapeutic medicine. 2014;8(4):1065-1074. ; Shi L, Zhao F, Zhu F, et al. Traditional Chinese Medicine Formula "Xiaofeng granules" suppressed gouty arthritis animal models and inhibited the proteoglycan degradation on chondrocytes induced by monosodium urate. J Ethnopharmacol. 2016;191:254-263. Contributed Indexing: Keywords: GA; icariin; inflammation; mechanism; monosodium urate Substance Nomenclature: 0 (Cytokines) ; 0 (Flavonoids) ; 0 (NF-kappa B) ; 0 (NLR Family, Pyrin Domain-Containing 3 Protein) ; 0 (Nlrp3 protein, rat) ; 268B43MJ25 (Uric Acid) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20201102 Date Completed: 20210707 Latest Revision: 20210707 Update Code: 20240513

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Icariin alleviates MSU-induced rat GA models through NF-κB/NALP3 pathway.