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We engineered Escherichia coli to grow on CO 2 and formic acid alone by introducing the synthetic CO 2 and formic acid assimilation pathway, expressing two formate dehydrogenase genes, fine-tuning metabolic fluxes and optimizing the levels of cytochrome bo 3 and bd-I ubiquinol oxidase. Our engineered strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO 2 and formic acid alone.

Titel:	Escherichia coli is engineered to grow on CO <subscript>2</subscript> and formic acid.
Autor/in / Beteiligte Person:	Bang, J ; Hwang, CH ; Ahn, JH ; Lee, JA ; Lee, SY
Link:	Zum Volltext
Zeitschrift:	Nature microbiology, Jg. 5 (2020-12-01), Heft 12, S. 1459
Veröffentlichung:	[London] : Nature Publishing Group, [2016]-, 2020
Medientyp:	academicJournal
ISSN:	2058-5276 (electronic)
DOI:	10.1038/s41564-020-00793-9
Schlagwort:	Escherichia coli growth & development Escherichia coli Proteins genetics Escherichia coli Proteins metabolism Formate Dehydrogenases genetics Formate Dehydrogenases metabolism Metabolic Engineering Oxidoreductases genetics Oxidoreductases metabolism Carbon Dioxide metabolism Escherichia coli genetics Escherichia coli metabolism Formates metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Nat Microbiol] 2020 Dec; Vol. 5 (12), pp. 1459-1463. <i>Date of Electronic Publication: </i>2020 Sep 28. MeSH Terms: Carbon Dioxide / metabolism ; Escherichia coli / genetics ; Escherichia coli / metabolism ; Formates / metabolism ; Escherichia coli / growth & development ; Escherichia coli Proteins / genetics ; Escherichia coli Proteins / metabolism ; Formate Dehydrogenases / genetics ; Formate Dehydrogenases / metabolism ; Metabolic Engineering ; Oxidoreductases / genetics ; Oxidoreductases / metabolism References: Kumar, A. et al. Enhanced CO 2 fixation and biofuel production via microalgae: recent developments and future directions. Trends Biotechnol. 28, 371–380 (2010). (PMID: 10.1016/j.tibtech.2010.04.004) ; Singh, A. K., Kishore, G. M. & Pakrasi, H. B. Emerging platforms for co-utilization of one-carbon substrates by photosynthetic organisms. Curr. Opin. Biotechnol. 53, 201–208 (2018). (PMID: 10.1016/j.copbio.2018.02.002) ; Tashiro, Y., Hirano, S., Matson, M. M., Atsumi, S. & Kondo, A. Electrical-biological hybrid system for CO 2 reduction. Metab. Eng. 47, 211–218 (2018). (PMID: 10.1016/j.ymben.2018.03.015) ; Yishai, O., Bouzon, M., Döring, V. & Bar-Even, A. In vivo assimilation of one-carbon via a synthetic reductive glycine pathway in Escherichia coli. ACS Synth. Biol. 7, 2023–2028 (2018). (PMID: 10.1021/acssynbio.8b00131) ; Döring, V., Darii, E., Yishai, O., Bar-Even, A. & Bouzon, M. Implementation of a reductive route of one-carbon assimilation in Escherichia coli through directed evolution. ACS Synth. Biol. 7, 2029–2036 (2018). (PMID: 10.1021/acssynbio.8b00167) ; Innocent, B. et al. Electro-reduction of carbon dioxide to formate on lead electrode in aqueous medium. J. Appl. Electrochem. 39, 227 (2009). (PMID: 10.1007/s10800-008-9658-4) ; Agarwal, A. S., Zhai, Y., Hill, D. & Sridhar, N. The electrochemical reduction of carbon dioxide to formate/formic acid: engineering and economic feasibility. ChemSusChem 4, 1301–1310 (2011). (PMID: 10.1002/cssc.201100220) ; Boddien, A. et al. CO 2 -“neutral” hydrogen storage based on bicarbonates and formates. Angew. Chem. Int. Ed. 50, 6411–6414 (2011). (PMID: 10.1002/anie.201101995) ; Bang, J. & Lee, S. Y. Assimilation of formic acid and CO 2 by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways. Proc. Natl Acad. Sci. USA 115, E9271–E9279 (2018). (PMID: 10.1073/pnas.1810386115) ; Gleizer, S. et al. Conversion of Escherichia coli to generate all biomass carbon from CO 2 . Cell 179, 1255–1263 (2019). (PMID: 10.1016/j.cell.2019.11.009) ; Kim, S. et al. Growth of E. coli on formate and methanol via the reductive glycine pathway. Nat. Chem. Biol. 16, 538–545 (2020). (PMID: 10.1038/s41589-020-0473-5) ; Jeong, K. J. & Lee, S. Y. Enhanced production of recombinant proteins in Escherichia coli by filamentation suppression. Appl. Environ. Microbiol. 69, 1295–1298 (2003). (PMID: 10.1128/AEM.69.2.1295-1298.2003) ; Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–345 (2009). (PMID: 10.1038/nmeth.1318) ; Song, C. W. & Lee, S. Y. Rapid one-step inactivation of single or multiple genes in Escherichia coli. Biotechnol. J. 8, 776–784 (2013). (PMID: 10.1002/biot.201300153) Substance Nomenclature: 0 (Escherichia coli Proteins) ; 0 (Formates) ; 0YIW783RG1 (formic acid) ; 142M471B3J (Carbon Dioxide) ; EC 1.- (Oxidoreductases) ; EC 1.10.99.- (ubiquinol oxidase) ; EC 1.17.1.9 (Formate Dehydrogenases) Entry Date(s): Date Created: 20200929 Date Completed: 20210226 Latest Revision: 20210824 Update Code: 20240513

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Escherichia coli is engineered to grow on CO <subscript>2</subscript> and formic acid.