Preliminary study of icariin indicating prevention of steroid-induced osteonecrosis of femoral head by regulating abnormal expression of miRNA-335 and protecting the functions of bone microvascular endothelial cells in rats.

Yue, J ; Yu, H ; et al.

In: Gene, Jg. 766 (2021-01-15), S. 145128

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Background: The pathogenesis of osteonecrosis of the femoral head (ONFH) is unclear. Our previous study demonstrated that upregulated miR-335 in bone microvascular endothelial cells (BMECs) might be associated with the disease of steroid-induced ONFH. Here, we study the preventive effect of ICA on steroid-induced ONFH in rats.

Method: 90 rats were separated into three groups: control group, methylprednisolone (MPS) group, and MPS + Icariin (ICA) group. Four weeks later, histological analyses were performed. Thrombomodulin (TM) and vascular endothelial growth factor (VEGF) were tested. MiRNA-335 expression was screened in the three groups using Agilent Gene Spring GX software. Target genes of miRNA-335 were detected by bioinformatics analysis. The functions of BMECs were analyzed by scratch, angiogenesis and cell survival rate.

Results: ICA can prevent the occurrence of steroid-associated ONFH in rats and reduce the amount of TM and VEGF in serum induced by glucocorticoids. ICA could regulate the overexpression of miRNA-335 induced by glucocorticoids. We predicted the Gene ontology (GO) and signaling pathways of target genes. At 24 hours, we found that ICA significantly promoted BMECs migration abilities. We also found that ICA could promote the angioplasty ability of BMECs. ICA could improve the survival rate of BMECs after steroid-induced injury.

Conclusions: ICA is effective to prevent the occurrence of steroidinduced ONFH. ICA has a protective effect against steroid-induced BMECs injury. ICA regulated the imbalance of miRNA-335 expression induced by the glucocorticoid in BMECs, which provides a new viewpoint to explore the mechanism of ICA in preventing steroid-induced ONFH.

Titel:	Preliminary study of icariin indicating prevention of steroid-induced osteonecrosis of femoral head by regulating abnormal expression of miRNA-335 and protecting the functions of bone microvascular endothelial cells in rats.
Autor/in / Beteiligte Person:	Yue, J ; Yu, H ; Liu, P ; Wen, P ; Zhang, H ; Guo, W ; Zhang, Q
Link:	Zum Volltext
Zeitschrift:	Gene, Jg. 766 (2021-01-15), S. 145128
Veröffentlichung:	Amsterdam, Elsevier/North-Holland, 1976-, 2021
Medientyp:	academicJournal
ISSN:	1879-0038 (electronic)
DOI:	10.1016/j.gene.2020.145128
Schlagwort:	Adipogenesis drug effects Animals Cell Movement drug effects Cell Survival drug effects Endothelial Cells metabolism Female Femur Head metabolism Femur Head Necrosis chemically induced Femur Head Necrosis metabolism Glucocorticoids metabolism Methylprednisolone pharmacology Neovascularization, Pathologic metabolism Osteocytes drug effects Osteogenesis drug effects Rats Rats, Sprague-Dawley Signal Transduction drug effects Steroids pharmacology Vascular Endothelial Growth Factor A metabolism Endothelial Cells drug effects Femur Head drug effects Femur Head Necrosis drug therapy Flavonoids pharmacology MicroRNAs metabolism Neovascularization, Pathologic drug therapy Protective Agents pharmacology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Gene] 2021 Jan 15; Vol. 766, pp. 145128. <i>Date of Electronic Publication: </i>2020 Sep 07. MeSH Terms: Endothelial Cells / drug effects ; Femur Head / drug effects ; Femur Head Necrosis / drug therapy ; Flavonoids / pharmacology ; MicroRNAs / metabolism ; Neovascularization, Pathologic / drug therapy ; Protective Agents / *pharmacology ; Adipogenesis / drug effects ; Animals ; Cell Movement / drug effects ; Cell Survival / drug effects ; Endothelial Cells / metabolism ; Female ; Femur Head / metabolism ; Femur Head Necrosis / chemically induced ; Femur Head Necrosis / metabolism ; Glucocorticoids / metabolism ; Methylprednisolone / pharmacology ; Neovascularization, Pathologic / metabolism ; Osteocytes / drug effects ; Osteogenesis / drug effects ; Rats ; Rats, Sprague-Dawley ; Signal Transduction / drug effects ; Steroids / pharmacology ; Vascular Endothelial Growth Factor A / metabolism Contributed Indexing: Keywords: Femoral head; Glucocorticoid; Icariin; Osteonecrosis Substance Nomenclature: 0 (Flavonoids) ; 0 (Glucocorticoids) ; 0 (MicroRNAs) ; 0 (Protective Agents) ; 0 (Steroids) ; 0 (Vascular Endothelial Growth Factor A) ; VNM47R2QSQ (icariin) ; X4W7ZR7023 (Methylprednisolone) Entry Date(s): Date Created: 20200910 Date Completed: 20201105 Latest Revision: 20201105 Update Code: 20240513

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