The expression of androstenone receptor (OR7D4) in vomeronasal organ and olfactory epithelium of horses.

Choi, Y ; Yoon, M

In: Domestic animal endocrinology, Jg. 74 (2021), S. 106535

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Androstenone is the first mammalian steroidal pheromone to be identified. Pheromones are chemicals that animals use to communicate within a species. Pheromone detections are related to vomeronasal organ (VNO) and olfactory epithelium (OE) in mammals. Olfactory Receptor Family 7 Subfamily D Member 4 (OR7D4) is an odorant receptor that responds to androstenone. Several studies indicated that spray with androstenone changes behaviors of the boar and dogs. However, the expression of OR7D4 in VNO and OE was not reported in mammals except human. Thus, the main objectives of this study were to investigate the expression of OR7D4 in VNO and OE of horses. Tissue samples were collected from the VNO and nasal cavity of 6 thoroughbred horses. The presence of OR7D4 gene was investigated with reverse transcription-polymerase chain reaction. The expression of OR7D4 was determined using Western blot and immunofluorescence. As a result, the bands for OR7D4 were observed at approximately 462 bp. The protein band of OR7D4 of VNO and OE was detected at 38 kDa. Immunofluorescence result showed that the cilia and cytoplasm of olfactory receptor cells of VNO and nasal cavity tissues were immunolabeled with OR7D4 antibody. The intensity of OR7D4 protein bands in the ventral region of the ethmoidal concha tissues was not significantly different between mares and geldings. In conclusion, thoroughbred horses are capable of androstenone perception through OR7D4 expressed in the VNO and OE.

Titel:	The expression of androstenone receptor (OR7D4) in vomeronasal organ and olfactory epithelium of horses.
Autor/in / Beteiligte Person:	Choi, Y ; Yoon, M
Link:	Zum Volltext
Zeitschrift:	Domestic animal endocrinology, Jg. 74 (2021), S. 106535
Veröffentlichung:	Stoneham, Ma : Elsevier ; <i>Original Publication</i>: [Auburn, Ala.] : Domendo, Inc., c1984-, 2021
Medientyp:	academicJournal
ISSN:	1879-0054 (electronic)
DOI:	10.1016/j.domaniend.2020.106535
Schlagwort:	Animals Female Gene Expression Regulation Male Polymerase Chain Reaction veterinary Receptors, Odorant genetics Horses physiology Olfactory Mucosa metabolism Receptors, Odorant metabolism Vomeronasal Organ metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Domest Anim Endocrinol] 2021 Jan; Vol. 74, pp. 106535. <i>Date of Electronic Publication: </i>2020 Aug 06. MeSH Terms: Horses / physiology ; Olfactory Mucosa / metabolism ; Receptors, Odorant / metabolism ; Vomeronasal Organ / metabolism ; Animals ; Female ; Gene Expression Regulation ; Male ; Polymerase Chain Reaction / veterinary ; Receptors, Odorant / genetics Contributed Indexing: Keywords: Androstenone; Horses; OR7D4; Olfactory epithelium; Vomeronasal organ Substance Nomenclature: 0 (Receptors, Odorant) Entry Date(s): Date Created: 20200908 Date Completed: 20211206 Latest Revision: 20211214 Update Code: 20240513

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