Icariin Protects Mouse Insulinoma Min6 Cell Function by Activating the PI3K/AKT Pathway.
In: Medical science monitor : international medical journal of experimental and clinical research, Jg. 26 (2020-09-04), S. e924453
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Zugriff:
BACKGROUND Type 2 diabetes (T2D) is characterized by ß-cell dysfunction and insulin resistance. Icariin (ICA), a flavonoid from Epimedium, possesses anti-diabetic and anti-inflammatory properties. However, it is unclear whether ICA acts on pancreatic ß-cells. The present study was designed to explore the effects and latent mechanism of ICA on uric acid (UA)-stimulated pancreatic b-cell dysfunction. MATERIAL AND METHODS Min6 cells were exposed to various concentrations of ICA for 24 h, and cell viability was assessed by MTT assays. Min6 cells were treated with ICA for 2 h, followed by 5 mg/dl UA for 24 h, and cell viability, apoptosis, apoptosis-associated protein levels and insulin secretion were assessed by MTT, flow cytometry, western blotting and glucose-stimulated insulin secretion assays, respectively. The effects of ICA and UA on the PI3K/Akt pathway were also analyzed by western blotting, as were the effects of the specific PI3K/Akt inhibitor LY294002. RESULTS ICA was not cytotoxic toward Min6 cells. UA decreased Min6 cell viability, enhanced cell apoptosis and levels of cleaved caspase-3, and reduced pro-caspase3 levels and insulin secretion, with all of these effects reversed by ICA in a dose-dependent manner. UA inhibited the PI3K/AKT pathway, an effect reversed by ICA treatment. The specific PI3K/Akt inhibitor LY294002, however, reversed these effects of ICA on UA-treated Min6 cells. CONCLUSIONS ICA protected Min6 cell function, an effect likely mediated by the PI3K pathway. ICA may inhibit the progression of diabetes.
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Icariin Protects Mouse Insulinoma Min6 Cell Function by Activating the PI3K/AKT Pathway.
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Autor/in / Beteiligte Person: | Zhang, T ; Qiu, F |
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Zeitschrift: | Medical science monitor : international medical journal of experimental and clinical research, Jg. 26 (2020-09-04), S. e924453 |
Veröffentlichung: | 2014- : Smithtown, NY : International Scientific Information, Inc. ; <i>Original Publication</i>: Warsaw, Poland : Medical Science International, 2020 |
Medientyp: | academicJournal |
ISSN: | 1643-3750 (electronic) |
DOI: | 10.12659/MSM.924453 |
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