The functioning of a novel protein, Swollenin, in promoting the lignocellulose degradation capacity of Trichoderma guizhouense NJAU4742 from a proteomic perspective.
In: Bioresource technology, Jg. 317 (2020-12-01), S. 123992
Online
academicJournal
Zugriff:
The functioning of a novel auxiliary enzyme, TgSWO from Trichoderma guizhouense NJAU4742, was investigated based on the proteomic analysis of wild-type (WT), knockout (KO) and overexpression (OE) treatments. The results showed that the cellulase and hemicellulase activities of OE and WT were significantly higher than those of KO. Simultaneously, tandem mass tag (TMT) analysis results indicated that cellulases and hemicellulases were significantly upregulated in OE, especially hydrophobin (HFB, A1A105805.1) and endo-β-1,4-glucanases (A1A101831.1), with ratios of 43.73 and 9.88, respectively, compared with WT. The synergistic effect of TgSWO on cellulases increased the reducing sugar content by 1.45 times in KO + TgSWO (1.8 mg) compared with KO, and there was no significant difference between KO + TgSWO (1.2 mg) and WT. This study elucidated the function of TgSWO in promoting the lignocellulose degradation capacity of NAJU4742, which provides new insights into the efficient conversion of lignocellulose.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
| Titel: |
The functioning of a novel protein, Swollenin, in promoting the lignocellulose degradation capacity of Trichoderma guizhouense NJAU4742 from a proteomic perspective.
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| Autor/in / Beteiligte Person: | Meng, X ; Ma, L ; Li, T ; Zhu, H ; Guo, K ; Liu, D ; Ran, W ; Shen, Q |
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| Zeitschrift: | Bioresource technology, Jg. 317 (2020-12-01), S. 123992 |
| Veröffentlichung: | Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-, 2020 |
| Medientyp: | academicJournal |
| ISSN: | 1873-2976 (electronic) |
| DOI: | 10.1016/j.biortech.2020.123992 |
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