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Background: Icariin has been shown to enhance bone formation.

Objective: The present study aimed to investigate whether icariin also promotes bone fracture healing and its mechanisms.

Methods: First, we isolated and cultured rat bone marrow stromal cells (rBMSCs) with icariincontaining serum at various concentrations (0%, 2.5%, 5% and 10%) and then measured alkaline phosphatase (ALP) activity and the expression of Core-binding factor, alpha 1 (Cbfα1), bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein-4 (BMP-4) in the rBMSCs. Second, we established a model of fracture healing in rats and performed gavage treatment for 20 days. Then, we detected bone biochemical markers (ELISA kits) in the serum, fracture healing (digital radiography, DR), and osteocalcin expression (immunohistochemistry).

Results: Icariin treatment increased ALP activity and induced the expression of Cbfα1, BMP-2 and BMP-4 in rBMSCs in a dose-dependent manner. In addition, Icariin increased the serum levels of osteocalcin (OC), bone-specific alkaline phosphatase (BAP), N-terminal telopeptides of type I collagen (NTX-1), C-terminal telopeptide of type I collagen (CTX-1) and tartrate-resistant acid phosphatase 5b (TRACP-5b); promoted osteocalcin secretion at the fracture site; and accelerated fracture healing.

Conclusion: Icariin can promote the levels of bone-formation markers, accelerate fracture healing, and activate the WNT1/β-catenin osteogenic signaling pathway.

Titel:	Icariin Accelerates Fracture Healing via Activation of the WNT1/β-catenin Osteogenic Signaling Pathway.
Autor/in / Beteiligte Person:	Zhang, XY ; Chen, YP ; Zhang, C ; Zhang, X ; Xia, T ; Han, J ; Yang, N ; Song, SL ; Xu, CH
Zeitschrift:	Current pharmaceutical biotechnology, Jg. 21 (2020), Heft 15, S. 1645
Veröffentlichung:	Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000-, 2020
Medientyp:	academicJournal
ISSN:	1873-4316 (electronic)
DOI:	10.2174/1389201021666200611121539
Schlagwort:	Alkaline Phosphatase metabolism Animals Cell Differentiation drug effects Cells, Cultured Disease Models, Animal Female Flavonoids administration & dosage Fractures, Bone metabolism Male Mesenchymal Stem Cells drug effects Mesenchymal Stem Cells metabolism Osteocalcin metabolism Rats Rats, Wistar Tibia drug effects Tibia metabolism Wnt1 Protein metabolism beta Catenin metabolism Flavonoids therapeutic use Fracture Healing drug effects Fractures, Bone drug therapy Osteogenesis drug effects Wnt Signaling Pathway drug effects
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Curr Pharm Biotechnol] 2020; Vol. 21 (15), pp. 1645-1653. MeSH Terms: Flavonoids / therapeutic use ; Fracture Healing / drug effects ; Fractures, Bone / drug therapy ; Osteogenesis / drug effects ; Wnt Signaling Pathway / *drug effects ; Alkaline Phosphatase / metabolism ; Animals ; Cell Differentiation / drug effects ; Cells, Cultured ; Disease Models, Animal ; Female ; Flavonoids / administration & dosage ; Fractures, Bone / metabolism ; Male ; Mesenchymal Stem Cells / drug effects ; Mesenchymal Stem Cells / metabolism ; Osteocalcin / metabolism ; Rats ; Rats, Wistar ; Tibia / drug effects ; Tibia / metabolism ; Wnt1 Protein / metabolism ; beta Catenin / metabolism Contributed Indexing: Keywords: ELISA kits; Icariin; WNT1/β-catenin; fracture healing; osteocalcin expression; rat bone marrow stromal cells Substance Nomenclature: 0 (Flavonoids) ; 0 (Wnt1 Protein) ; 0 (Wnt1 protein, rat) ; 0 (beta Catenin) ; 104982-03-8 (Osteocalcin) ; EC 3.1.3.1 (Alkaline Phosphatase) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20200612 Date Completed: 20210107 Latest Revision: 20210107 Update Code: 20240513

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Icariin Accelerates Fracture Healing via Activation of the WNT1/β-catenin Osteogenic Signaling Pathway.