Multicenter Evaluation of the Xpert Carba-R Assay for Detection and Identification of Carbapenemase Genes in Sputum Specimens.
In: Journal of clinical microbiology, Jg. 58 (2020-08-24), Heft 9
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Zugriff:
Rapid diagnosis of infections caused by carbapenem-resistant Enterobacteriaceae (CRE) is crucial for proper treatment and infection control. The Xpert Carba-R assay is a qualitative multiplex real-time PCR method that qualitatively detects and differentiates five common carbapenemase genes ( bla KPC , bla NDM , bla VIM , bla OXA-48 , and bla IMP ) directly from rectal swabs or purified colonies within approximately 1 h. We performed a multicenter evaluation of the investigational use of the Carba-R assay for detection and differentiation of carbapenemase genes from sputum specimens in patients with a clinical diagnosis of pneumonia. The intra- and interassay coefficients of variation values for the Carba-R assay were 0.2% to 2.0% and 1.4% to 2.3%, respectively. A total of 301 sputum specimens were collected and tested. Compared to bacterial culture followed by PCR identification of resistance genes from colonies, the Carba-R assay reduced turnaround time from 56 to 84 h to less than 2 h. Carbapenemase genes were detected by the Carba-R assay in Klebsiella pneumoniae ( n = 236), Escherichia coli ( n = 22), Enterobacter cloacae ( n = 23), Klebsiella oxytoca ( n = 8), Serratia marcescens ( n = 6), Citrobacter freundii ( n = 4), and Klebsiella aerogenes ( n = 2). The Carba-R assay detected 112 bla KPC (33.5%), 70 bla NDM (21.0%), 8 bla IMP (2.4%), and 2 bla VIM (0.6%) genes, with positive percent agreement, negative percent agreement, and concordance rates of 92.9%, 86.7%, and 88.3%, respectively, for the dominant bla KPC and 85.0%, 87.8%, and 87.4%, respectively, for the bla NDM genes. Neither method detected the bla OXA-48 carbapenemase gene. The convenient, rapid, and simple characteristics of the Xpert Carba-R assay make it a potential tool for CRE detection and identification directly in sputum specimens.
(Copyright © 2020 American Society for Microbiology.)
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Multicenter Evaluation of the Xpert Carba-R Assay for Detection and Identification of Carbapenemase Genes in Sputum Specimens.
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Autor/in / Beteiligte Person: | Cai, Z ; Tao, J ; Jia, T ; Fu, H ; Zhang, X ; Zhao, M ; Du, H ; Yu, H ; Shan, B ; Huang, B ; Chen, L ; Tang, YW ; Jia, W ; Qu, F |
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Zeitschrift: | Journal of clinical microbiology, Jg. 58 (2020-08-24), Heft 9 |
Veröffentlichung: | Washington, American Society for Microbiology., 2020 |
Medientyp: | academicJournal |
ISSN: | 1098-660X (electronic) |
DOI: | 10.1128/JCM.00644-20 |
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