Einzeltreffer — DigiBib

Continuous recording of intracellular activities in single cells is required for deciphering rare, dynamic and heterogeneous cell responses, which are missed by population or brief single-cell recording. Even if the field of intracellular recording is constantly proceeding, several technical challenges are still remained to conquer this important approach. Here, we demonstrate long-term intracellular recording by combining a vertical nanowire multi electrode array (VNMEA) with optogenetic stimulation to minimally disrupt cell survival and functions during intracellular access and measurement. We synthesized small-diameter and high-aspect-ratio silicon nanowires to spontaneously penetrate into single cells, and used light to modulate the cell's responsiveness. The light-induced intra- and extracellular activities of individual optogenetically-modified cells were measured simultaneously, and each cell showed distinctly different measurement characteristics according to the cell-electrode configuration. Intracellular recordings were achieved continuously and reliably without signal interference and attenuation over 24 hours. The integration of two controllable techniques, vertically grown nanowire electrodes and optogenetics, expands the strategies for discovering the mechanisms for crucial physiological and dynamic processes in various types of cells.

Titel:	Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array.
Autor/in / Beteiligte Person:	Yoo, J ; Kwak, H ; Kwon, J ; Ha, GE ; Lee, EH ; Song, S ; Na, J ; Lee, HJ ; Lee, J ; Hwangbo, A ; Cha, E ; Chae, Y ; Cheong, E ; Choi, HJ
Link:	Zum Volltext
Zeitschrift:	Scientific reports, Jg. 10 (2020-03-09), Heft 1, S. 4279
Veröffentlichung:	London : Nature Publishing Group, copyright 2011-, 2020
Medientyp:	academicJournal
ISSN:	2045-2322 (electronic)
DOI:	10.1038/s41598-020-61325-3
Schlagwort:	HEK293 Cells Humans Action Potentials Cell Physiological Phenomena Electrodes Nanowires chemistry Optogenetics Silicon chemistry
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Sci Rep] 2020 Mar 09; Vol. 10 (1), pp. 4279. <i>Date of Electronic Publication: </i>2020 Mar 09. MeSH Terms: Action Potentials* ; Cell Physiological Phenomena* ; Electrodes* ; Optogenetics* ; Nanowires / chemistry ; Silicon / chemistry ; HEK293 Cells ; Humans References: Skylaki, S., Hilsenbeck, O. & Schroeder, T. Challenges in long-term imaging and quantification of single-cell dynamics. Nat. Biotechnol. 34, 1137–1144 (2016). (PMID: 10.1038/nbt.3713) ; VanDersarl, J. J. & Renaud, P. Biomimetic surface patterning for long-term transmembrane access. Sci. Rep. 6, 1–10 (2016). (PMID: 10.1038/srep32485) ; Dhawale, A. K. et al. Automated long-term recording and analysis of neural activity in behaving animals. Elife 6, 1–40 (2017). (PMID: 10.7554/eLife.27702) ; Dipalo, M. et al. Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes. Nano Lett. 17, 3932–3939 (2017). (PMID: 10.1021/acs.nanolett.7b01523) ; Xie, C., Lin, Z., Hanson, L., Cui, Y. & Cui, B. Intracellular recording of action potentials by nanopillar electroporation. Nat. Nanotechnol. 7, 185–190 (2012). (PMID: 10.1038/nnano.2012.8) ; Lin, Z. C., Xie, C., Osakada, Y., Cui, Y. & Cui, B. Iridium oxide nanotube electrodes for sensitive and prolonged intracellular measurement of action potentials. Nat. Commun. 5, 1–10 (2014). ; Park, H. et al. Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits. Nat. Nanotechnol. 7, 180–184 (2012). (PMID: 10.1038/nnano.2011.249) ; Abbott, J. et al. CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging. Nat. Nanotechnol. 12, 460–466 (2017). (PMID: 10.1038/nnano.2017.3) ; Hai, A. & Spira, M. E. On-chip electroporation, membrane repair dynamics and transient in-cell recordings by arrays of gold mushroom-shaped microelectrodes. Lab Chip 12, 2865–2873 (2012). (PMID: 10.1039/c2lc40091j) ; Hai, A., Shappir, J. & Spira, M. E. In-cell recordings by extracellular microelectrodes. Nat. Methods 7, 200–2 (2010). (PMID: 10.1038/nmeth.1420) ; Hai, A., Shappir, J. & Spira, M. E. Long-Term, Multisite, Parallel, In-Cell Recording and Stimulation by an Array of Extracellular Microelectrodes. J. Neurophysiol. 104, 559–568 (2010). (PMID: 10.1152/jn.00265.2010) ; Nogan, J. et al. High Density Individually Addressable Nanowire Arrays Record Intracellular Activity from Primary Rodent and Human Stem Cell Derived Neurons. Nano Lett. 17, 2757–2764 (2017). (PMID: 10.1021/acs.nanolett.6b04752) ; Banghart, M., Borges, K., Isacoff, E., Trauner, D. & Kramer, R. H. Light-activated ion channels for remote control of neuronal firing. Nat. Neurosci. 7, 1381–1386 (2004). (PMID: 10.1038/nn1356) ; Tay, A., Kunze, A., Murray, C. & Carlo, D. Di. Induction of Calcium Influx in Cortical Neural Networks by Nanomagnetic Forces. ACS Nano 10, 2331–2341 (2016). (PMID: 10.1021/acsnano.5b07118) ; Abbott, J., Ye, T., Ham, D. & Park, H. Optimizing Nanoelectrode Arrays for Scalable Intracellular Electrophysiology. Acc. Chem. Res. 51, 600–608 (2018). (PMID: 10.1021/acs.accounts.7b00519) ; Gao, R. et al. Outside looking in: Nanotube transistor intracellular sensors. Nano Lett. 12, 3329–3333 (2012). (PMID: 10.1021/nl301623p) ; Duan, X. et al. Intracellular recordings of action potentials by an extracellular nanoscale field-effect transistor. Nat. Nanotechnol. 7, 174–179 (2012). (PMID: 10.1038/nnano.2011.223) ; Laxpati, N. G. et al. Real-time in vivo optogenetic neuromodulation and multielectrode electrophysiologic recording with NeuroRighter. Front. Neuroeng. 7, 1–15 (2014). (PMID: 10.3389/fneng.2014.00040) ; Malyshev, A., Goz, R., LoTurco, J. J. & Volgushev, M. Advantages and limitations of the use of optogenetic approach in studying fast-scale spike encoding. PLoS One 10, 1–17 (2015). (PMID: 10.1371/journal.pone.0122286) ; Schoeters, R. et al. Comparison between Direct Electrical and Optogenetic Subthalamic Nucleus Stimulation. EMF-Med 2018 - 1st EMF-Med World Conf. Biomed. Appl. Electromagn. Fields COST EMF-MED Final Event with 6th MCM 1, 1–2 (2018). ; Ratnadurai-Giridharan, S., Cheung, C. C. & Rubchinsky, L. L. Effects of electrical and optogenetic deep brain stimulation on synchronized oscillatory activity in Parkinsonian basal ganglia. IEEE Trans. Neural Syst. Rehabil. Eng. 25, 2188–2195 (2017). (PMID: 10.1109/TNSRE.2017.2712418) ; Entcheva, E. & Williams, J. C. Channelrhodopsin2 Current During the Action Potential: ‘Optical AP Clamp’ and Approximation. Sci. Rep. 4 (2014). ; Williams, J. C. & Entcheva, E. Optogenetic versus electrical stimulation of human cardiomyocytes: Modeling insights. Biophys. J. 108, 1934–1945 (2015). (PMID: 10.1016/j.bpj.2015.03.032) ; Ayling, O. G. S., Harrison, T. C., Boyd, J. D., Goroshkov, A. & Murphy, T. H. Automated light-based mapping of motor cortex by photoactivation of channelrhodopsin-2 transgenic mice. Nat. Methods 6, 219–224 (2009). (PMID: 10.1038/nmeth.1303) ; Cardin, J. A. et al. Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. Nat. Protoc. 5, 247–254 (2010). (PMID: 10.1038/nprot.2009.228) ; Woong, K., Jennifer, K. N., Kunitake, M. E., Conklin, B. R. & Yang, P. Interfacing Silicon Nanowires with Mammalian Cells. J. Am. Chem. Soc. 129, 7228–7229 (2007). (PMID: 10.1021/ja071456k) ; Shalek, A. K. et al. Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells. Proc. Natl. Acad. Sci. 107, 1870–1875 (2010). (PMID: 10.1073/pnas.0909350107) ; Lee, K. Y. et al. Coupling of semiconductor nanowires with neurons and their interfacial structure. Nanoscale Res. Lett. 5, 410–415 (2010). (PMID: 10.1007/s11671-009-9498-0) ; Lee, K. Y. et al. Vertical nanowire probes for intracellular signaling of living cells. Nanoscale Res. Lett. 9, 1–7 (2014). (PMID: 10.1186/1556-276X-9-1) ; Kim, H., Kim, I., Choi, H. J., Kim, S. Y. & Yang, E. G. Neuron-like differentiation of mesenchymal stem cells on silicon nanowires. Nanoscale 7, 17131–17138 (2015). (PMID: 10.1039/C5NR05787F) ; Kim, I. et al. Enhanced Neurite Outgrowth by Intracellular Stimulation. Nano Lett. 15, 5414–5419 (2015). (PMID: 10.1021/acs.nanolett.5b01810) ; Kim, I. et al. Large current difference in Au-coated vertical silicon nanowire electrode array with functionalization of peptides. Nanoscale Res. Lett. 8, 1–7 (2013). (PMID: 10.1186/1556-276X-8-1) ; Gunaydin, L. A. et al. Ultrafast optogenetic control. Nat. Neurosci. 13, 387–392 (2010). (PMID: 10.1038/nn.2495) ; Baek, H. et al. ZnO nanotube waveguide arrays on graphene films for local optical excitation on biological cells. APL Mater. 5 (2017). Substance Nomenclature: Z4152N8IUI (Silicon) Entry Date(s): Date Created: 20200311 Date Completed: 20201123 Latest Revision: 20210309 Update Code: 20240513 PubMed Central ID: PMC7062878

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array.