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Objectives: To investigate the effect of icariin (ICA) on early β-defensin-2 and T cell subsets in rats after tracheotomy.

Methods: A total of 54 SPF male Sprague-Dawley rats were randomly divided into a normal control group (group A), a model group (group B), and a model+ICA treatment group (group C), with 18 rats in each group. A tracheotomy intubation model of the B and C group was prepared. After 6 h of surgery, ICA intervention was given to group C. Groups A and B were given the same amount of normal saline. Lung tissue, alveolar lavage fluid and peripheral blood were taken at 24 h, 72 h and 168 h, respectively. The expression of rat β-defensin-2 mRNA in lung tissue was detected by RT-PCR. The content of β-defensin-2 in alveolar lavage fluid and peripheral blood serum was detected by ELISA. The content of peripheral blood T cell subsets (CD3 + , CD4 + , CD8 + ) was detected by flow cytometry, and the ratio of CD4 + /CD8 + was calculated.

Results: After tracheotomy, the levels of β-defensin-2 mRNA and β-defensin-2 in lung tissue from the group B were increased significantly at 24 h, then they were decreased gradually, and decreased most significantly at 168 h ( P <0.05). The content of β-defensin-2 in peripheral blood of group B decreased gradually, and the content of β-defensin-2 in 168 h was significantly lower than that in 24 h ( P <0.05), but there was no significant difference between group B and group A ( P >0.05). The level of CD3 + T cells in peripheral blood was significantly lower than that in the group A ( P <0.05), but their was no significant difference in CD4 + and CD8 + T cells compared with group A ( P >0.05). After ICA intervention in group C: lung tissue, alveolar lavage fluid, peripheral blood serum β-defensin-2 content, and peripheral blood CD3 + and CD4 + T cell levels were gradually increased, significantly higher than those in the group B ( P <0.05). CD8 + T cell level was significantly lower than that in the group A at 24 h ( P <0.05), the CD4 + /CD8 + ratio was significantly higher at 168 h than those in the group A or B (both P <0.01).

Conclusions: ICA can improve the early lung immune function in rats with tracheotomy, which might be related to up-regulation of β-defensin-2 in lung tissue and alveolar lavage fluid, concomitant with increases in CD3 + and CD4 + T cells and CD4 + /CD8 + ratio in peripheral blood while reduction in CD8 + cells.

Titel:	Effect of icariin on early β - defensin - 2 and T cell subsets in rats after tracheotomy.
Autor/in / Beteiligte Person:	Ju, J ; Tan, R ; Zhong, Y ; Peng, L ; Pan, Y
Link:	Zum Volltext
Zeitschrift:	Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, Jg. 45 (2020-01-28), Heft 1, S. 1
Veröffentlichung:	Changsha Shi : "Zhong nan da xue xue bao (yi xue ban)" bian ji bu, 2004-, 2020
Medientyp:	academicJournal
ISSN:	1672-7347 (print)
DOI:	10.11817/j.issn.1672-7347.2020.180754
Schlagwort:	Animals Flavonoids Male Rats Rats, Sprague-Dawley Tracheotomy beta-Defensins T-Lymphocyte Subsets
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Transliterated Title: · ARTICLES · ·论著· . Publication Type: Journal Article Language: English; Chinese [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2020 Jan 28; Vol. 45 (1), pp. 1-7. MeSH Terms: T-Lymphocyte Subsets* ; Animals ; Flavonoids ; Male ; Rats ; Rats, Sprague-Dawley ; Tracheotomy ; beta-Defensins References: 马艳芳. ICU气管切开患者肺部感染的危险因素分析及干预对策[J]. 中华医院感染学杂志, 2013, 23(12): 2807- 2808. ; Zhai YK, Guo X, Pan YL, et al. A systematic review of the efficacy and pharmacological profile of herba epimedii in osteoporosis therapy[J]. Pharmazie, 2013, 68(9): 713- 722. ; Gui Y, Zhang J, Chen L, et al. Icariin, a flavonoid with anti-cancer effects, alleviated paclitaxel-induced neuropathic pain in a SIRT1-dependent manner[J]. Mol Pain, 2018, 14: 174480691876897. ; Gandhi GR, MTSL Neta, Sathiyabama RG, et al. Flavonoids as Th1/Th2 cytokines immunomodulators: A systematic review of studies on animal models[J]. 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Grant Information: 81460687 United States US United States; 2018nb0211┫。This work was supported by the National Natural Science Foundation ┣81460687 United States US United States; 2018nb0211 Yongchuan District Natural Science Foundation Contributed Indexing: Keywords: T cell subset; early lung immune function; icariin; tracheotomy model; β-defensin-2 ; Local Abstract: [Publisher, Chinese] 目的 : 探讨淫羊藿苷(icariin，ICA)对气管切开模型大鼠早期免疫相关指标的影响。方法 : 将54只SPF级雄性SD大鼠随机分成正常对照组(A组)、模型组(B组)、模型+ICA治疗组(C组)，每组18只。B，C组制备大鼠气管切开模型。模型成功6 h后给予C组ICA干预，A，B组给予等量生理盐水。分别在第 24，72，168 h取肺组织、肺泡灌洗液及外周血。用RT-PCR检测大鼠肺组织中β-防御素-2 mRNA表达，ELISA检测肺泡灌洗液及外周血血清中β-防御素-2含量，流式细胞仪检测外周血T细胞亚群(CD3 + ，CD4 + ，CD8 + )含量，并计算CD4 + /CD8 + 值。结果 : 气管切开后B组大鼠肺组织β-防御素-2 mRNA及肺泡灌洗液β-防御素-2含量在24 h明显升高，随后逐渐降低，至168 h降低最明显(均 P <0.05)；外周血血清β-防御素-2含量逐渐降低，168 h明显低于24 h( P <0.05)，但3个时间点与A组比较差异均无统计学意义(均 P >0.05)；外周血CD3 + T细胞水平24 h明显低于A组( P <0.05)，CD4 + 和CD8 + T细胞水平3个时间点与A组比较差异均无统计学意义，且各组组内差异亦均无统计学意义(均 P >0.05)。C组ICA干预后：肺组织、肺泡灌洗液、外周血血清β-防御素-2含量及外周血中CD3 + 和CD4 + T细胞水平均逐渐升高，明显高于B组(均 P <0.05)；CD8 + 在24 h明显低于A组( P <0.05)，其余时间点3组组间组内比较差异均无统计学意义(均 P >0.05)；CD4 + /CD8 + 值在168 h明显高于A，B组(均 P <0.01)。结论 : ICA可以改善气管切开模型大鼠早期肺部免疫功能，这可能与上调肺组织β-防御素-2 mRNA表达及肺泡灌洗液、外周血血清中β-防御素-2含量，提高外周血中CD3 + 和CD4 + T细胞水平和CD4 + /CD8 + 值，降低CD8 + T细胞水平有关。. Substance Nomenclature: 0 (Flavonoids) ; 0 (beta-Defensins) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20200306 Date Completed: 20200309 Latest Revision: 20200309 Update Code: 20240513

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Effect of icariin on early β - defensin - 2 and T cell subsets in rats after tracheotomy.