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Background: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis.

Methods: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells.

Results: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

Titel:	Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor.
Autor/in / Beteiligte Person:	Sun, LJ ; Li, C ; Wen, XH ; Guo, L ; Guo, ZF ; Liao, LQ ; Guo, Y
Zeitschrift:	Current pharmaceutical biotechnology, Jg. 22 (2021), Heft 1, S. 168
Veröffentlichung:	Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000-, 2021
Medientyp:	academicJournal
ISSN:	1873-4316 (electronic)
DOI:	10.2174/1389201021666200123102550
Schlagwort:	Alkaline Phosphatase metabolism Animals Cell Line Collagen Type I metabolism Humans Osteoblasts cytology Osteoblasts metabolism Osteogenesis drug effects RNA, Messenger metabolism Cell Differentiation drug effects Cell Proliferation drug effects Flavonoids pharmacology Osteoblasts drug effects Osteoprotegerin metabolism RANK Ligand metabolism Receptors, Estrogen metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Curr Pharm Biotechnol] 2021; Vol. 22 (1), pp. 168-175. MeSH Terms: Cell Differentiation / drug effects ; Cell Proliferation / drug effects ; Flavonoids / pharmacology ; Osteoblasts / drug effects ; Osteoprotegerin / metabolism ; RANK Ligand / metabolism ; Receptors, Estrogen / *metabolism ; Alkaline Phosphatase / metabolism ; Animals ; Cell Line ; Collagen Type I / metabolism ; Humans ; Osteoblasts / cytology ; Osteoblasts / metabolism ; Osteogenesis / drug effects ; RNA, Messenger / metabolism Contributed Indexing: Keywords: Icariin; OPG/RANKL; differentiation; hFOB1.19; osteoblast; proliferation Substance Nomenclature: 0 (Collagen Type I) ; 0 (Flavonoids) ; 0 (Osteoprotegerin) ; 0 (RANK Ligand) ; 0 (RNA, Messenger) ; 0 (Receptors, Estrogen) ; 0 (TNFRSF11B protein, human) ; 0 (TNFSF11 protein, human) ; EC 3.1.3.1 (Alkaline Phosphatase) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20200124 Date Completed: 20210208 Latest Revision: 20210208 Update Code: 20240513

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Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor.