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Background: Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO 2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P. tricornutum and its simultaneous rapid growth rate under high-CO 2 (0.15%) cultivation.

Results: In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P. tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO 2 cultivation resulted in a much higher of both lipid content and growth in P. tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO 2 cultivated G6PDH overexpressed strains.

Conclusions: The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P. tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO 2 cultivated G6PDH overexpressed P. tricornutum suggested this G6PDH overexpression-high CO 2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production.

Titel:	Elevated CO <subscript>2</subscript> improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum.
Autor/in / Beteiligte Person:	Wu, S ; Gu, W ; Huang, A ; Li, Y ; Kumar, M ; Lim, PE ; Huan, L ; Gao, S ; Wang, G
Link:	Zum Volltext
Zeitschrift:	Microbial cell factories, Jg. 18 (2019-09-23), Heft 1, S. 161
Veröffentlichung:	London : BioMed Central, [2002-, 2019
Medientyp:	academicJournal
ISSN:	1475-2859 (electronic)
DOI:	10.1186/s12934-019-1214-x
Schlagwort:	Carbon Dioxide analysis Diatoms metabolism Genetic Engineering Glucosephosphate Dehydrogenase metabolism Microalgae genetics Microalgae growth & development Microalgae metabolism NADP metabolism Pentose Phosphate Pathway Photosynthesis Carbon Dioxide metabolism Diatoms genetics Diatoms growth & development Fatty Acids metabolism Glucosephosphate Dehydrogenase genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Microb Cell Fact] 2019 Sep 23; Vol. 18 (1), pp. 161. <i>Date of Electronic Publication: </i>2019 Sep 23. MeSH Terms: Carbon Dioxide / metabolism ; Diatoms / genetics ; Diatoms / growth & development ; Fatty Acids / metabolism ; Glucosephosphate Dehydrogenase / *genetics ; Carbon Dioxide / analysis ; Diatoms / metabolism ; Genetic Engineering ; Glucosephosphate Dehydrogenase / metabolism ; Microalgae / genetics ; Microalgae / growth & development ; Microalgae / metabolism ; NADP / metabolism ; Pentose Phosphate Pathway ; Photosynthesis References: Eur J Pharmacol. 2000 May 26;397(1):1-9. (PMID: 10844092) ; Science. 2001 Jul 13;293(5528):290-3. (PMID: 11452122) ; Planta. 2001 Mar;212(4):499-507. (PMID: 11525506) ; Planta. 2001 Mar;212(4):627-34. (PMID: 11525521) ; Plant Physiol. 2002 Feb;128(2):760-9. (PMID: 11842179) ; Trends Biochem Sci. 2002 Sep;27(9):467. (PMID: 12217522) ; Curr Opin Plant Biol. 2003 Jun;6(3):236-46. (PMID: 12753973) ; Biochemistry. 2006 Oct 31;45(43):13046-53. (PMID: 17059221) ; Biotechnol Adv. 2007 May-Jun;25(3):294-306. (PMID: 17350212) ; J Exp Bot. 2008;59(7):1569-80. (PMID: 18436543) ; J Plant Physiol. 2009 Jan 30;166(2):203-12. (PMID: 18541338) ; Planta. 2009 Mar;229(4):803-10. (PMID: 19112580) ; Proc Natl Acad Sci U S A. 2009 May 12;106(19):8061-6. (PMID: 19416911) ; Nucleic Acids Res. 2009 Aug;37(14):e96. (PMID: 19487243) ; Plant Mol Biol. 2010 Nov;74(4-5):493-502. (PMID: 20878347) ; Biotechnol Biofuels. 2012 Jun 06;5(1):40. (PMID: 22672912) ; Plant Biotechnol J. 2013 Feb;11(2):157-68. (PMID: 23066823) ; PLoS One. 2012;7(12):e51590. (PMID: 23236517) ; Appl Microbiol Biotechnol. 2013 Apr;97(7):2761-72. (PMID: 23420268) ; Biotechnol Biofuels. 2013 May 04;6(1):67. (PMID: 23642220) ; J Phys Chem B. 2013 Jun 13;117(23):6888-95. (PMID: 23688343) ; Appl Microbiol Biotechnol. 2013 Aug;97(15):7049-59. (PMID: 23771779) ; Mar Drugs. 2013 Nov 22;11(11):4662-97. (PMID: 24284429) ; Metab Eng. 2014 Mar;22:3-9. (PMID: 24333273) ; Sci Rep. 2014 Feb 04;4:3958. (PMID: 24492482) ; Trends Biotechnol. 2014 Mar;32(3):117-24. (PMID: 24529448) ; Plant Cell. 2014 Apr 25;26(4):1681-1697. (PMID: 24769481) ; Plant Cell Physiol. 2014 Aug;55(8):1395-403. (PMID: 24793748) ; Microb Cell Fact. 2014 Aug 09;13(1):100. (PMID: 25106441) ; Metab Eng. 2015 Jan;27:1-9. (PMID: 25447640) ; Metab Eng. 2015 May;29:1-11. (PMID: 25662836) ; Metab Eng. 2015 Jul;30:27-39. (PMID: 25747307) ; Biotechnol Biofuels. 2015 May 28;8:78. (PMID: 26052345) ; N Biotechnol. 2016 Jan 25;33(1):237-44. (PMID: 26162893) ; Sci Rep. 2016 Feb 18;6:21245. (PMID: 26887288) ; Metab Eng. 2017 May;41:212-221. (PMID: 28465173) ; Plant Biotechnol J. 2018 Jan;16(1):298-309. (PMID: 28605577) ; FASEB J. 1994 Feb;8(2):174-81. (PMID: 8119488) ; Biochem J. 1998 Mar 1;330 ( Pt 2):777-84. (PMID: 9480890) Grant Information: 41806171 National Natural Science Foundation of China; 41806158 National Natural Science Foundation of China Contributed Indexing: Keywords: Algal growth rate; Antisense knockdown; CO2; Glucose-6-phosphate dehydrogenase; Lipid accumulation; Overexpression; Phaeodactylum tricornutum Substance Nomenclature: 0 (Fatty Acids) ; 142M471B3J (Carbon Dioxide) ; 53-59-8 (NADP) ; EC 1.1.1.49 (Glucosephosphate Dehydrogenase) Entry Date(s): Date Created: 20190925 Date Completed: 20191008 Latest Revision: 20200225 Update Code: 20240513 PubMed Central ID: PMC6757359

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Elevated CO <subscript>2</subscript> improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum.