Absorption and utilisation of epimedin C and icariin from Epimedii herba, and the regulatory mechanism via the BMP2/ Runx2 signalling pathway.

Huang, X ; Wang, X ; et al.

In: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jg. 118 (2019-10-01), S. 109345

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Bone nonunion remains a challenge during the treatment of bone defect accompanied with infection. Epimedii herba is a widely used medicine in clinic to enhance bone healing. Epimedin C and icariin are the major flavonoid glycosides from Epimedii herba. Although the effects of these compounds have been reported, their different absorption and utilisation by osteoblasts remain unclear. In the present study, lipopolysaccharide (LPS)-induced osteoblast was adopted as the model cell to evaluate the effects of epimedin C and icariin. The intracellular and extracellular drug concentrations within 24 h were assayed by pipette tip solid-phase extraction and high-performance liquid chromatography, respectively. MTT, alkaline phosphatase (ALP) and calcified nodule staining were performed to identify and evaluate the effects of epimedin C and icariin on LPS-induced osteoblasts. The regulatory roles of epimedin C and icariin in the bone morphogenetic protein-2 (BMP-2)/Runt-related transcription factor 2 (Runx2) signalling pathway were investigated. The results revealed that epimedin C and icariin were not efficiently absorbed by LPS-induced osteoblasts. Nevertheless, they still had high utilisation efficiency after entering the cells. ALP activity, mineralisation and osteoblasts proliferation were enhanced by a high concentration of epimedin C and icariin. The suppressed expression of BMP-2 and Runx2 in LPS-induced osteoblasts was up-regulated significantly after treatment with epimedin C and icariin. These findings firstly illustrated the behavior of epimedin C and icariin from Epimedii herba on LPS-induced osteoblasts and the regulatory property on the expression of key genes and proteins of the BMP-2/Runx2 signalling pathway, which might be helpful for better understanding flavonoids' mechanism to enhance bone repair and improving their future application.

Titel:	Absorption and utilisation of epimedin C and icariin from Epimedii herba, and the regulatory mechanism via the BMP2/ Runx2 signalling pathway.
Autor/in / Beteiligte Person:	Huang, X ; Wang, X ; Zhang, Y ; Shen, L ; Wang, N ; Xiong, X ; Zhang, L ; Cai, X ; Shou, D
Link:	Zum Volltext
Zeitschrift:	Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jg. 118 (2019-10-01), S. 109345
Veröffentlichung:	Paris : Editions Scientifiques Elsevier ; <i>Original Publication</i>: New York, N.Y. : Masson Pub. USA, Inc., c1982-, 2019
Medientyp:	academicJournal
ISSN:	1950-6007 (electronic)
DOI:	10.1016/j.biopha.2019.109345
Schlagwort:	Animals Flavonoids chemistry Flavonoids pharmacology Lipopolysaccharides Male Osteoblasts drug effects Osteoblasts metabolism RNA, Messenger genetics RNA, Messenger metabolism Rats, Sprague-Dawley Bone Morphogenetic Protein 2 metabolism Core Binding Factor Alpha 1 Subunit metabolism Flavonoids metabolism Signal Transduction
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Biomed Pharmacother] 2019 Oct; Vol. 118, pp. 109345. <i>Date of Electronic Publication: </i>2019 Aug 19. MeSH Terms: Signal Transduction* ; Bone Morphogenetic Protein 2 / metabolism ; Core Binding Factor Alpha 1 Subunit / metabolism ; Flavonoids / *metabolism ; Animals ; Flavonoids / chemistry ; Flavonoids / pharmacology ; Lipopolysaccharides ; Male ; Osteoblasts / drug effects ; Osteoblasts / metabolism ; RNA, Messenger / genetics ; RNA, Messenger / metabolism ; Rats, Sprague-Dawley Contributed Indexing: Keywords: Absorption and utilisation; Bone morphogenetic protein-2/runt-related transcription factor 2 signalling pathway; Epimedii herba; Lipopolysaccharide; Osteoblast Substance Nomenclature: 0 (Bone Morphogenetic Protein 2) ; 0 (Core Binding Factor Alpha 1 Subunit) ; 0 (Flavonoids) ; 0 (Lipopolysaccharides) ; 0 (RNA, Messenger) ; 110642-44-9 (epimedin C) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20190924 Date Completed: 20200204 Latest Revision: 20200204 Update Code: 20231215

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