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Infertility caused by environmental pollution is becoming a global problem, but an effective prevention or treatment is lacking. Icariin (ICA) is a flavonoid used in traditional Chinese medicine. The present study investigated the possible roles of ICA in preventing testicular dysfunction caused by di(2-ethylhexyl) phthalate (DEHP), one of the most studied environmental endocrine disruptors. Cultured mouse Leydig cells were pretreated with ICA and exposed to DEHP to determine ICA effects upon cell proliferation, reactive oxygen species (ROS) levels, mitochondrial membrane potential (Δψm), testosterone levels and the expression of transcription factor SF-1 and steroidogenic enzymes (CYP11, 3β-HSD and 17β-HSD), which play critical roles in androgen production. Our results showed that ICA reversed the adverse effect of DEHP on Leydig cell proliferation, and decreased ROS levels and elevated Δψm levels. Also, ICA promoted testosterone production and up-regulated the expression of SF-1 and steroidogenic enzymes. We investigated ICA actions in vivo, using male mice administrated DEHP followed by ICA. Exposure to DEHP decreased epididymal sperm counts and disrupted seminiferous tubules, and both of these effects were reversed by ICA treatment. These results showed that the mechanisms of ICA in protecting mouse testes against DEHP-induced damage involves the prevention of ROS accumulation and promotion of testosterone secretion.

Titel:	Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di(2-ethylhexyl) phthalate.
Autor/in / Beteiligte Person:	Sun, J ; Wang, D ; Lin, J ; Liu, Y ; Xu, L ; Lv, R ; Mo, K ; Lian, X ; Xie, M ; Xu, S ; Wang, S
Link:	Zum Volltext
Zeitschrift:	Toxicology and applied pharmacology, Jg. 378 (2019-09-01), S. 114612
Veröffentlichung:	New York, NY : Academic Press ; <i>Original Publication</i>: New York., 2019
Medientyp:	academicJournal
ISSN:	1096-0333 (electronic)
DOI:	10.1016/j.taap.2019.114612
Schlagwort:	Animals Cell Proliferation drug effects Endocrine Disruptors metabolism Female Leydig Cells metabolism Male Mice Mice, Inbred ICR Pregnancy Prenatal Exposure Delayed Effects metabolism Reactive Oxygen Species metabolism Spermatozoa drug effects Spermatozoa metabolism Testis drug effects Testis metabolism Diethylhexyl Phthalate adverse effects Flavonoids pharmacology Leydig Cells drug effects Phthalic Acids adverse effects Protective Agents pharmacology Testosterone metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Toxicol Appl Pharmacol] 2019 Sep 01; Vol. 378, pp. 114612. <i>Date of Electronic Publication: </i>2019 Jun 06. MeSH Terms: Diethylhexyl Phthalate / adverse effects ; Flavonoids / pharmacology ; Leydig Cells / drug effects ; Phthalic Acids / adverse effects ; Protective Agents / pharmacology ; Testosterone / metabolism ; Animals ; Cell Proliferation / drug effects ; Endocrine Disruptors / metabolism ; Female ; Leydig Cells / metabolism ; Male ; Mice ; Mice, Inbred ICR ; Pregnancy ; Prenatal Exposure Delayed Effects / metabolism ; Reactive Oxygen Species / metabolism ; Spermatozoa / drug effects ; Spermatozoa / metabolism ; Testis / drug effects ; Testis / metabolism Contributed Indexing: Keywords: Icariin; Leydig cells; Mouse; Testes; di(2-Ethylhexyl) Phthalate (DEHP) Substance Nomenclature: 0 (Endocrine Disruptors) ; 0 (Flavonoids) ; 0 (Phthalic Acids) ; 0 (Protective Agents) ; 0 (Reactive Oxygen Species) ; 3XMK78S47O (Testosterone) ; 6O7F7IX66E (phthalic acid) ; C42K0PH13C (Diethylhexyl Phthalate) ; VNM47R2QSQ (icariin) Entry Date(s): Date Created: 20190609 Date Completed: 20200520 Latest Revision: 20200520 Update Code: 20240513

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Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di(2-ethylhexyl) phthalate.